Macrolide-resistant Streptococcus pyogenes in Central Greece: prevalence; mechanism and molecular identification

Malli, Ergina; Tatsidou, Evi; Damani, A.; Pantelidi, Kleoniki; Petinaki, Efthimia; Skoulakis, Charalampos; Δρούγκα, Ελεάννα; Spiliopoulou, Iris

doi:10.1016/j.ijantimicag.2010.01.025

Cited by 18 publications

(17 citation statements)

References 6 publications

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“…It has been suggested that changes in resistance in a given population may be partly due to the spreading of resistant clones [18]. In contrast to our previous study, where mefA was found to be the predominant resistance mechanism, there was no marked difference in the frequency of mefA as compared to ermB in the current study.…”

Section: Discussioncontrasting

confidence: 99%

Significant decline in the erythromycin resistance of group A streptococcus isolates at a German paediatric tertiary care centre

Farmand

Henneke

Hufnagel

et al. 2011

Eur J Clin Microbiol Infect Dis

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Group A streptococcus (GAS) is considered to be a major pathogen of bacterial tonsillopharyngitis in children. Although GAS is generally susceptible to penicillin, macrolides are often used as the second-line treatment. Over the last several decades, the rising macrolide resistance of GAS has been detected in several countries. With the current study, we aimed to determine the development of macrolide resistance at our paediatric centre. From March 2006 to May 2009, 350 GAS isolates were tested for susceptibility to erythromycin, azithromycin, clindamycin, penicillin and cefotaxime. Macrolide-resistant isolates were screened for the presence of genes related to macrolide resistance (mefA, ermB, ermTR, prtF1). In comparison to a prior study at our hospital, the erythromycin resistance rate decreased significantly from 13.6 to 2.6%. This effect may be attributable to a more restrictive use of macrolides in children in our region.

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Section: Discussioncontrasting

confidence: 99%

Significant decline in the erythromycin resistance of group A streptococcus isolates at a German paediatric tertiary care centre

Farmand

Henneke

Hufnagel

et al. 2011

Eur J Clin Microbiol Infect Dis

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“…High macrolide resistance in GAS was reported in most southern European countries, such as Spain, Greece, Italy, and Portugal (14,15,19,22,25,27), but this was not a characteristic of all European countries (22). Although differences in the prevalence of particular macrolide resistance genotypes and emm types were documented, the majority of these emm types shared the same resistance determinants, suggesting a broad geographical dissemination of a few clones.…”

Section: S Treptococcus Pyogenes (Lancefield Group a Streptococci [Gas])mentioning

confidence: 94%

Differences between Macrolide-Resistant and -Susceptible Streptococcus pyogenes: Importance of Clonal Properties in Addition to Antibiotic Consumption

Silva-Costa

Friães

Ramírez

et al. 2012

Antimicrob Agents Chemother

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A steady decline in macrolide resistance among Streptococcus pyogenes (group A streptococci [GAS]) in Portugal was reported during 1999 to 2006. This was accompanied by alterations in the prevalence of macrolide resistance phenotypes and in the clonal composition of the population. In order to test whether changes in the macrolide-resistant population reflected the same changing patterns of the overall population, we characterized both macrolide-susceptible and -resistant GAS associated with a diagnosis of tonsillo-pharyngitis recovered in the period from 2000 to 2005 in Portugal. Pulsed-field gel electrophoresis (PFGE) profiling was the best predictor of emm type and the only typing method that could discriminate clones associated with macrolide resistance and susceptibility within each emm type. Six PFGE clusters were significantly associated with macrolide susceptibility: T3-emm3-ST406, T4-emm4-ST39, T1-emm1-ST28, T6-emm6-ST382, B3264-emm89-ST101/ST408, and T2-emm2-ST55. Four PFGE clusters were associated with macrolide resistance: T4-emm4-ST39, T28-emm28-ST52, T12-emm22-ST46, and T1-emm1-ST28. We found no evidence for frequent ongoing horizontal transfer of macrolide resistance determinants. The diversity of the macrolide-resistant population was lower than that of susceptible isolates. The differences found between the two populations suggest that the macrolide-resistant population of GAS has its own dynamics, independent of the behavior of the susceptible population.

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“…Erythromycin and related macrolide antibiotics, which are used in patients with a known or suspected allergy to penicillin, are considered as alternative drugs [2, 10, 11]. Unfortunately, an increasing incidence of erythromycin resistance has been reported in several parts of the world in recent years [12–15]. Moreover, no vaccines are now available to prevent streptococcal infections and their sequelae.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity ofRhodomyrtus tomentosa(Aiton) Hassk. Leaf Extract against Clinical Isolates ofStreptococcus pyogenes

Limsuwan

Kayser

Voravuthikunchai

2012

Evidence-Based Complementary and Alternative Medicine

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Ethanol extract of Rhodomyrtus tomentosa (Aiton) Hassk. leaf was evaluated for antibacterial activity against 47 clinical isolates of Streptococcus pyogenes. The extract exhibited good anti-S. pyogenes activity against all the tested isolates with similar minimum inhibitory concentration (MIC, 3.91–62.5 μg mL−1) and minimum bactericidal concentration (MBC, 3.91–62.5 μg mL−1) ranges. No surviving cells were detected at 16 h after treatment with 8 × MIC of the extract. The extract-treated cells demonstrated no lysis and cytoplasmic leakage through the bacterial membrane. Electron micrographs further revealed that the extract did not cause any dramatic changes on the treated cells. Rhodomyrtone, an isolated compound, exhibited good anti-S. pyogenes activity (14 isolates), expressed very low MIC (0.39–1.56 μg mL−1) and MBC (0.39-1.56 μg mL−1) values. Rhodomyrtus tomentosa leaf extract and rhodomyrtone displayed promising antibacterial activity against clinical isolates of S. pyogenes.

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Macrolide-resistant Streptococcus pyogenes in Central Greece: prevalence; mechanism and molecular identification

Cited by 18 publications

References 6 publications

Significant decline in the erythromycin resistance of group A streptococcus isolates at a German paediatric tertiary care centre

Significant decline in the erythromycin resistance of group A streptococcus isolates at a German paediatric tertiary care centre

Differences between Macrolide-Resistant and -Susceptible Streptococcus pyogenes: Importance of Clonal Properties in Addition to Antibiotic Consumption

Antibacterial Activity ofRhodomyrtus tomentosa(Aiton) Hassk. Leaf Extract against Clinical Isolates ofStreptococcus pyogenes

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