Macromol. Biosci. 3/2011

Nagase, Kenichi; Watanabe, Minami; Kikuchi, Akihiko; Yamato, Masayuki; Okano, Teruo

doi:10.1002/mabi.201190008

Cited by 16 publications

(23 citation statements)

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“…In many studies, the cell detachment process was found to be slow and needed 1 h or more in a static condition [41][42][43]. Patel found that it even took nearly 3 h for the cells to completely detach from the commercially available thermo-responsive surface UpCell ® [44].…”

Section: Temperature-dependent Cell-detachment Behaviormentioning

confidence: 99%

Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Zhuang

Liu

Song

et al. 2015

Materials Science and Engineering: C

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Section: Temperature-dependent Cell-detachment Behaviormentioning

confidence: 99%

Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Zhuang

Liu

Song

et al. 2015

Materials Science and Engineering: C

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“…Cell sheet transplantation has advantages compared with these other methods. Cell sheets are fabricated in a thermoresponsive cell culture dish, and can then be recovered simply by changing temperature, without trypsinization, so that the extracellular matrix on the basal surface of the cell sheets is preserved . The extracellular matrix acts as a tissue‐adhesive glue.…”

Section: Introductionmentioning

confidence: 99%

Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Nagase

Nagumo

Kim

et al. 2017

Macromolecular Bioscience

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Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)‐releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic‐co‐glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six‐layered cardiomyocyte sheets are transplanted with a VEGF‐releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF‐releasing mat, and incorporate larger‐diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF‐releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF‐releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.

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“…7 Stimuli-responsive polymers have been applied in drug delivery, tissue engineering, and biosensors. [13][14][15][16] Among the applications of thermoresponsive polymers, cell sheet engineering has received increasing attention. [13][14][15][16] Among the applications of thermoresponsive polymers, cell sheet engineering has received increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Targeted grafting of thermoresponsive polymers from a penetrative honeycomb structure for cell sheet engineering

2015

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Responsive membranes have been used to construct smart biomaterial interfaces. We report a novel approach to fabricate honeycomb films with a pattern of thermoresponsive polymer, namely poly(N-isopropylacrylamide). The approach was based on a combination of the breath figure method and reversible addition-fragmentation chain transfer. The hybrid film had morphological and chemical patterns resulting in varied wettability and morphology at various stages, as well as high thermo-responsiveness. Enhanced cell adhesion was observed at an incubation temperature of 37 °C, which is above its lower critical solution temperature (LCST). Furthermore, cells could be harvested at temperatures below the LCST without trypsin treatment. The non-invasive characteristics give this membrane potential as a substrate for cell sheet engineering.

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Macromol. Biosci. 3/2011

Cited by 16 publications

References 0 publications

Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Targeted grafting of thermoresponsive polymers from a penetrative honeycomb structure for cell sheet engineering

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