Macrophage-Derived Chemokine (MDC/CCL22) and CCR4 Are Involved in the Formation of T Lymphocyte-Dendritic Cell Clusters in Human Inflamed Skin and Secondary Lymphoid Tissue

Katou, Fuminori; Ohtani, Haruo; Nakayama, Takashi; Ono, Koji; Matsushima, Kouji; Saaristo, Anne; Nishimura, Hiroshi; Yoshie, Osamu; Motegi, Katsutoshi

doi:10.1016/s0002-9440(10)64077-1

Cited by 94 publications

(78 citation statements)

References 30 publications

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“…Thus, even though the concentrations of CCL28 found free in saliva and milk are relatively low compared with its IC 50 for various microbes, its high surface concentrations on the mucosal surfaces together with synergistic effects with other antimicrobial factors rich in mucosal secretions (33) may provide a highly effective barrier protection against a wide spectrum of microbes. Notably, autologous skin flaps transplanted into the oral cavity to reconstruct large tissue defects after radical resection of oral cancer are known to be frequently infected with C. albicans (23). Skin surfaces, which may be less effectively covered by CCL28 than normal oral mucosa, may be easily colonized by C. albicans even in the presence of high concentrations of histatins and other antimicrobial agents in the saliva.…”

Section: Discussionmentioning

confidence: 99%

“…Written informed consents were obtained from both patients. Immunohistochemistry was conducted as described previously (23). In brief, periodate-lysine-4% paraformaldehyde-prefixed frozen sections were reacted with mouse monoclonal anti-CCL28 (clone no.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…This was conducted by adopting the pre-embedding immunoperoxidase method as described previously (23). In brief, sections adjacent to those used for immunohistochemistry were processed the same way, except that 0.3% H 2 O 2 /0.1% NaN 3 in PBS was used after reaction with primary Ab.…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

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CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity

Hieshima¹,

Ohtani

Shibano³

et al. 2003

The Journal of Immunology

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232

268

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CCL28 is a CC chemokine signaling via CCR10 and CCR3 that is selectively expressed in certain mucosal tissues such as exocrine glands, trachea, and colon. Notably, these tissues commonly secrete low-salt fluids. RT-PCR analysis demonstrated that salivary glands expressed CCL28 mRNA at the highest levels among various mouse tissues. Single cells prepared from mouse parotid glands indeed contained a major fraction of CD3−B220low cells that expressed CCR10 at high levels and CCR3 at low levels and responded to CCL28 in chemotaxis assays. Morphologically, these cells are typical plasma cells. By immunohistochemistry, acinar epithelial cells in human and mouse salivary glands were strongly positive for CCL28. Furthermore, human saliva and milk were found to contain CCL28 at high concentrations. Moreover, the C terminus of human CCL28 has a significant sequence similarity to histatin-5, a histidine-rich candidacidal peptide in human saliva. Subsequently, we demonstrated that human and mouse CCL28 had a potent antimicrobial activity against Candida albicans, Gram-negative bacteria, and Gram-positive bacteria. The C-terminal 28-aa peptide of human CCL28 also displayed a selective candidacidal activity. In contrast, CCL27, which is most similar to CCL28 and shares CCR10, showed no such potent antimicrobial activity. Like most other antimicrobial peptides, CCL28 exerted its antimicrobial activity in low-salt conditions and rapidly induced membrane permeability in target microbes. Collectively, CCL28 may play dual roles in mucosal immunity as a chemoattractant for cells expressing CCR10 and/or CCR3 such as plasma cells and also as a broad-spectrum antimicrobial protein secreted into low-salt body fluids.

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

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CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity

Hieshima¹,

Ohtani

Shibano³

et al. 2003

The Journal of Immunology

Self Cite

232

268

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“…Thus, the expressions of IL-4, CCR4, and CCR8 may require allergen stimulation in the inflamed region. In addition, TARC and MDC are expressed not only in airway epithelium but also in the other organs such as skin (31,32) and the nose (33). Taken together, it is suggested that T cells in the peripheral blood, especially Th2-type cells, of asthmatic patients migrate from blood into bronchial tissue without inflammatory stimulation by a mechanism other than chemokines such as MDC and TARC and their ligands of CCR4.…”

Section: Discussionmentioning

confidence: 99%

T Cells of Atopic Asthmatics Preferentially Infiltrate Into Human Bronchial Xenografts in SCID Mice

Tsumori

Kohrogi

Goto

et al. 2003

The Journal of Immunology

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T cells play an important role in the pathogenesis of bronchial asthma. However, it is not completely known how circulating lymphocytes infiltrate into the airways of asthmatic patients. Because SCID mice are unable to reject xenogenic transplants, many xenotransplant models using various human tissues have been developed. Therefore, to examine the interaction between bronchi and T lymphocytes of asthma, it may be possible to use the human bronchial xenograft and PBMC xenograft in SCID mice. We transplanted human bronchi into the subcutaneum of SCID mice and i.p. injected PBMCs that were obtained from patients with atopic asthma, atopic dermatitis and rheumatoid arthritis, and normal subjects (asthmatic, dermatitis, rheumatic, and normal huPBMC-SCID mice). There was no difference in the percentage of CD3-, CD4-, CD8-, CD25-, CD45RO-, CD103-, and cutaneous lymphocyte Ag-positive cells in PBMCs among the patients with asthma, dermatitis, rheumatoid arthritis, and normal subjects, and CD3-positive cells in peripheral blood of asthmatic, dermatitis, rheumatic, and normal huPBMC-SCID mice. The number of CD3-, CD4-, and CD8-positive cells in the xenografts of asthmatic huPBMC-SCID mice was higher than those of dermatitis, rheumatic, and normal huPBMC-SCID mice. IL-4 mRNA and IL-5 mRNA were significantly higher in the xenografts of asthmatic huPBMC-SCID mice than those in the xenografts of normal huPBMC-SCID mice, but there were no significant differences in the expressions of IL-2 mRNA or IFN-γ mRNA between them. These findings suggest that T cells, especially Th2-type T cells, of asthmatics preferentially infiltrate into the human bronchi.

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“…On the other hand, whether DCs can fully mature depends on their ability to undergo in vivo migration. The in vivo migration of DCs is a complicated and successive process in which a series of chemokines and accessory receptors play critical roles (29,30). Thus, we investigated the impact of CD40/CD40L costimulatory signal on the function of mDCs and DC-activated T cells as well as their chemokine secretion and chemokine receptor expression.…”

Section: Discussionmentioning

confidence: 99%

Distinct Effect of CD40 and TNF-Signaling on the Chemokine/Chemokine Receptor Expression and Function of the Human Monocyte-Derived Dendritic Cells

Xia

Jun

et al. 2008

Cell Mol Immunol

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Macrophage-Derived Chemokine (MDC/CCL22) and CCR4 Are Involved in the Formation of T Lymphocyte-Dendritic Cell Clusters in Human Inflamed Skin and Secondary Lymphoid Tissue

Abstract: Chemokines are small secreted proteins that play critical roles in the migration of various types of leukocytes. Some chemokines are also likely to be involved in the interactions between T cells and dendritic cells (DC).

Cited by 94 publications

References 30 publications

CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity

CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity

T Cells of Atopic Asthmatics Preferentially Infiltrate Into Human Bronchial Xenografts in SCID Mice

Distinct Effect of CD40 and TNF-Signaling on the Chemokine/Chemokine Receptor Expression and Function of the Human Monocyte-Derived Dendritic Cells

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