Macrophage-fibroblast interaction and its possible role in the regulation of collagen metabolism during wound healing

Шехтер, А. Б.; Berchenko, G N; Николаев, А. В.

doi:10.1007/bf00800769

Cited by 8 publications

(1 citation statement)

References 4 publications

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“…Lung macrophages can phagocytose Cd particles, and both Cd and metallothionein (MT), a cysteine-rich Cd-binding protein, can accumulate within the lung macrophages (18,19). Macrophages secrete many factors that modify extracellular matrix (ECM) deposition and collagen metabolism (20), highlighting the critical role of cell-cell interaction in lung fibrosis (21). We have demonstrated that Cd induces -smooth muscle actin (-SMA) activation, ECM protein accumulation, and collagen secretion, suggesting that Cd is fibrogenic, in which fibroblast differentiation and ECM deposition play a critical role (7).…”

Section: Introductionmentioning

confidence: 99%

Citrullinated vimentin mediates development and progression of lung fibrosis

Surolia

et al. 2021

Sci. Transl. Med.

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The mechanisms by which environmental exposures contribute to the pathogenesis of lung fibrosis are unclear. Here, we demonstrate an increase in cadmium (Cd) and carbon black (CB), common components of cigarette smoke (CS) and environmental particulate matter (PM), in lung tissue from subjects with idiopathic pulmonary fibrosis (IPF). Cd concentrations were directly proportional to citrullinated vimentin (Cit-Vim) amounts in lung tissue of subjects with IPF. Cit-Vim amounts were higher in subjects with IPF, especially smokers, which correlated with lung function and were associated with disease manifestations. Cd/CB induced the secretion of Cit-Vim in an Akt1- and peptidylarginine deiminase 2 (PAD2)–dependent manner. Cit-Vim mediated fibroblast invasion in a 3D ex vivo model of human pulmospheres that resulted in higher expression of CD26, collagen, and α-SMA. Cit-Vim activated NF-κB in a TLR4-dependent fashion and induced the production of active TGF-β1, CTGF, and IL-8 along with higher surface expression of TLR4 in lung fibroblasts. To corroborate ex vivo findings, mice treated with Cit-Vim, but not Vim, independently developed a similar pattern of fibrotic tissue remodeling, which was TLR4 dependent. Moreover, wild-type mice, but not PAD2−/− and TLR4 mutant (MUT) mice, exposed to Cd/CB generated high amounts of Cit-Vim, in both plasma and bronchoalveolar lavage fluid, and developed lung fibrosis in a stereotypic manner. Together, these studies support a role for Cit-Vim as a damage-associated molecular pattern molecule (DAMP) that is generated by lung macrophages in response to environmental Cd/CB exposure. Furthermore, PAD2 might represent a promising target to attenuate Cd/CB-induced fibrosis.

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Section: Introductionmentioning

confidence: 99%

Citrullinated vimentin mediates development and progression of lung fibrosis

Surolia

et al. 2021

Sci. Transl. Med.

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Phenytoin‐induced connective tissue growth in the rat

1986

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Rats were treated daily for 9 days with 100, 50, or 25 mg/kg phenytoin i.p. This treatment resulted in a significant increase in the thickness of the connective tissue capsules of the liver, spleen, and pancreas, and of the subepithelial connective tissue of the mesentery but not the epicardium or visceral pleura of the lung where exposure to the drug was via the vascular route. Many areas of connective tissue growth exhibited obvious proliferation of fibroblasts and in some areas contained seemingly large numbers of macrophages and an increase in vascularity. It was demonstrated by electron microscopy that the macrophages occasionally were seen in intimate contact with the fibroblasts. Our observations clearly showed that intraperitoneal exposure of visceral connective tissues of the rat to phenytoin rapidly resulted in a dose-related proliferation of that tissue. The presence of numerous macrophages leads to the suggestion that macrophage-derived growth factor could be responsible for the increased growth.

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Scanning Electron Microscopy of Macrophages

Carr¹

1980

Morphology

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Macrophage-fibroblast interaction and its possible role in the regulation of collagen metabolism during wound healing

Cited by 8 publications

References 4 publications

Citrullinated vimentin mediates development and progression of lung fibrosis

Citrullinated vimentin mediates development and progression of lung fibrosis

Phenytoin‐induced connective tissue growth in the rat

Scanning Electron Microscopy of Macrophages

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