Macrophage-like RAW 264 cell line and time-resolved fluoroimmunoassay (TRFIA) as tools in screening drug effects on cytokine secretion

Pennanen, Niina; Lapinjoki, Seppo; Palander, Anne; Urtti, Arto; Mönkkönen, Jukka

doi:10.1016/0192-0561(95)00030-6

Cited by 38 publications

(22 citation statements)

References 16 publications

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“…It has been well-documented that various cytokines from the interferon family, such as IFN-␣, IFN-␤, and IFN-␥, play important roles in protection against HSV-2 (4, 6, 10, 18, 20-22, 32). There have also been studies showing TNF-␣ as a potent antiviral cytokine (24,27,30). Based on these findings, we investigated various cytokines to determine whether any of them could be responsible for the protection in vivo.…”

Section: Discussionmentioning

confidence: 99%

Induction of Innate Immunity against Herpes Simplex Virus Type 2 Infection via Local Delivery of Toll-Like Receptor Ligands Correlates with Beta Interferon Production

Gill

Deacon

Lichty

et al. 2006

J Virol

101

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Toll-like receptors (TLRs) constitute a family of innate receptors that recognize and respond to a wide spectrum of microorganisms, including fungi, bacteria, viruses, and protozoa. Previous studies have demonstrated that ligands for TLR3 and TLR9 induce potent innate antiviral responses against herpes simplex virus type 2 (HSV-2). However, the factor(s) involved in this innate protection is not well-defined. Here we report that production of beta interferon (

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Section: Discussionmentioning

confidence: 99%

Induction of Innate Immunity against Herpes Simplex Virus Type 2 Infection via Local Delivery of Toll-Like Receptor Ligands Correlates with Beta Interferon Production

Gill

Deacon

Lichty

et al. 2006

J Virol

101

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“…The assay is described in detail elsewhere [14]. In brief, the microtiter strips (Nunc, Roskilde, Denmark) were coated with the capture antibody, 1 gg/ml of IL-6 (Pharmingen, San Diego, CA), or 2 gg/ml of TNFa (Pharmingen, San Diego, CA), and the non-specific binding was blocked with 1% BSA in 50mM Tris-HCl-buffer containing 150 mM NaC1 (pH 7.5).…”

Section: Analysis Of Ll-6 and Tnfc~mentioning

confidence: 99%

“…The murine macrophage-like RAW 264 cell-line was grown in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Grand Island, NY), supplemented with 10% FCS and 100 IU/ml penicillin and streptomycin, in 7% CO2 atmosphere at 37 °C The cell culture experiments were carried out as previously described [14]. Briefly, in 6 the cytokine and NO experiments the cells were diluted to 10 cells/ ml and dispensed to 96-well cell culture plates (Nunc, Roskilde, Denmark), 200gl/well (2 x 105 cells/well), and in the iNOS experiments the cells were dispensed to 6-well plates, 2ml/well (2 x 106 cells/well).…”

Section: Cell Culture and The Induction Of The Cytokines And Nomentioning

confidence: 99%

The effect of free gallium and gallium in liposomes on cytokine and nitric oxide secretion from macrophage-like cells in vitro

Makkonen

Hirvonen²,

Savolainen

et al. 1995

Inflamm Res

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The aim of this study was to evaluate the effect of gallium nitrate, gallium-nitrilotriacetate (NTA) complex, and liposomal gallium-NTA on IL-6, TNF alpha, and nitric oxide (NO) release from activated macrophages. In addition, the expression of the inducible nitric oxide synthase (iNOS) was determined. Gallium inhibited dose-dependently the secretion of IL-6, TNF alpha, and NO from the LPS-induced macrophage-like RAW 264 cells. Encapsulation of gallium in negatively charged DSPG-liposomes increased its potency 10-50 times and 7-11 times compared to free gallium nitrate and gallium-NTA, respectively. Neither non-loaded liposomes nor NTA alone inhibited cytokine or NO secretion, demonstrating that the observed effects originated from gallium. Liposomal gallium-NTA inhibited the expression of iNOS by the macrophages, while other formulations of gallium had no effect. Thus, gallium, when delivered properly, suppresses macrophage functions by inhibiting the release of inflammatory mediators from the cells.

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“…Leukocytes present in the exudates were measured using a Coulter counter. After centrifugation of exudates, the supernatants were used to assay bilirubin (Turcanu et al, 1998), LTB 4 , and PGE 2 levels by radioimmunoassay (Moroney et al, 1988), nitrite by a fluorometric method (Misko et al, 1993), cytokine levels by timeresolved fluoroimmunoassay (Pennanen et al, 1995), and histamine by enzyme immunoassay. Cell pellets were used for Western blot analysis or nitric-oxide synthase-2 (NOS-2) activity assay by the L-[2,3,4,5-3 H]arginine method, as reported previously (Posadas et al, 2000).…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of Heme Oxygenase-1 Up-Regulation in the Development of Experimental Inflammation Induced by Zymosan

Vicente¹,

Guillén²,

Habib³

et al. 2003

J Pharmacol Exp Ther

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Heme oxygenase-1 (HO-1) is part of the integrated response to oxidative stress. This enzyme may exert anti-inflammatory effects in some animal models, although the precise mechanisms are not fully understood. We have examined the role of HO-1 in the inflammatory response induced by zymosan in the mouse air pouch. Zymosan administration induced HO-1 protein expression in leukocytes migrating to exudates, with maximal levels in the late phase of this response (24 -48 h). This was accompanied by ferritin induction and bilirubin accumulation, indicating that this enzyme is active in our model. HO-1 expression by zymosan treatment was partly reduced by aminoguanidine, suggesting the participation of endogenous nitric oxide in the mechanisms leading to HO-1 synthesis in the zymosaninjected mouse air pouch. Up-regulation of HO-1 by hemin administration resulted in inhibition of nitric-oxide synthase-2 activity, cellular infiltration into the air pouch exudate, and plasmatic exudation. Leukotriene B 4 levels in exudates were significantly decreased in the early phase of this response (4 h), whereas interleukin-1␤ and tumor necrosis factor-␣ were inhibited at all time points. Inhibition of HO-1 activity by zinc protoporphyrin IX prevented most of the effects caused by hemin administration. Our results indicate that HO-1 exerts anti-inflammatory effects on the response to zymosan in the mouse air pouch and support a role for this enzyme in the modulation of inflammatory processes.

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Macrophage-like RAW 264 cell line and time-resolved fluoroimmunoassay (TRFIA) as tools in screening drug effects on cytokine secretion

Cited by 38 publications

References 16 publications

Induction of Innate Immunity against Herpes Simplex Virus Type 2 Infection via Local Delivery of Toll-Like Receptor Ligands Correlates with Beta Interferon Production

Induction of Innate Immunity against Herpes Simplex Virus Type 2 Infection via Local Delivery of Toll-Like Receptor Ligands Correlates with Beta Interferon Production

The effect of free gallium and gallium in liposomes on cytokine and nitric oxide secretion from macrophage-like cells in vitro

Beneficial Effects of Heme Oxygenase-1 Up-Regulation in the Development of Experimental Inflammation Induced by Zymosan

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