Macrophage Serum‐Based Adhesion to Plasma‐Processed Surface Chemistry is Distinct from That Exhibited by Fibroblasts

Abstract

Plasma-polymerized films deposited from AlAm, HxAm, NVP, NVFA, AA and FC were compared to TCPS and PS surfaces in supporting cellular attachment, viability, and proliferation in serum-based culture in vitro for extended periods of time (>7 d). Surface patterns were created using multi-step depositions with physical masks. Cell adhesion in the presence of serum was compared for (monocyte-) macrophage and fibroblast cell lines. Cellular response was tracked over time, reporting adhesive behavior, proliferative rates, and morphological changes as a function of surface chemistry. Micropatterned surfaces containing different surface chemistries and functional groups (e.g. -NH(2), -COOH, -CF(3)) produced differential cell adhesive patterns for NIH 3T3 fibroblasts compared to J774A.1, RAW 264.7 or IC-21 (monocyte-) macrophage cell types. Significantly, macrophage adhesion is substantial on surfaces where fibroblasts do not adhere under identical culture conditions.

Minimal In Vitro Antimicrobial Efficacy and Ocular Cell Toxicity from Silver Nanoparticles

“…In the murine ELISA datasets for RAW264.7 macrophages, nanoparticle-treated cell IL-1β and IL-6 expression was often at or above that of non-treated control cells and notably equivalent to LPS treatment in RAW264.7 cells, except for IL-6 at 12 and 24 h. However, these results were not statistically significant. Even so, macrophage-activated cytokine expression might be expected at higher levels than epithelial cell cytokine expression because of the immunomodulating phenotype of the macrophage, intrinsic reactivity to particles [32,[51][52][53], and expected reaction to nanoparticle exposure in this case. In support of this, nanoparticle-treated HCE-T cell IL-1β, IL-6, and IL-8 levels were either at or below that of controls.…”

“…RAW264.7 cells were dissociated from culture flasks by incubation with Ca 2+ -and Mg 2+ -free cell culture grade Hank's balanced salt solution (HBSS, Life Technologies). All RAW264.7 subcultures were used before passage 15 as received from ATCC [32]. HCE cells were used between five and 15 passages and routinely passaged using 0.25% trypsin/0.1% ethylenediaminetetraacetic acid (EDTA; Mediatech).…”

Conformal Encapsulation of Three-Dimensional, Bioresorbable Polymeric Scaffolds Using Plasma-Enhanced Chemical Vapor Deposition

“…It is well established that ECM proteins required for cell attachment and proliferation adsorb in significantly lower quantities on hydrophobic materials, limiting or inhibiting cell adhesion and proliferation, 38,39 although exceptions do exist. 40 These results suggest that FC treated scaffolds are excellent candidates for 3D low-fouling, bio-nonreactive materials that are noncytotoxic.…”

Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates

“…24 Using mammalian cells, the presence of amine groups is regarded as being more important than hydrophilicity, 19,24,25 and more enhanced adhesion of endothelial cells has been shown on a surface with cationic groups than one with carboxyl groups. 26 The study of Griesser et al also showed that hydrophilicity and N content on tissue culture plates have a strong effect on the growth of the human cell, and that the amide group is the main inducer of cell attachment.…”