Macrophage spreading in vitro

Rabinovitch, M.; DeStefano, Mary Jo

doi:10.1016/0014-4827(73)90584-3

Cited by 144 publications

(19 citation statements)

References 25 publications

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“…Then, the cells were stained with a 5% solution of Giemsa and examined under microscopy where the percentage of spread cells were determined under 40ϫ magnification. Spread cells were those that presented cytoplasmic elongation, while the non-spread cells were rounded, [33][34][35][36] see Fig. 4.…”

Section: Count Of the Total Number Of Cells Present In The Peritonealmentioning

confidence: 99%

Synergystic Antitumor Effect of Polyphenolic Components of Water Soluble Derivative of Propolis against Ehrlich Ascites Tumour

Oršolić

Kosalec

Basić

2005

Biological & Pharmaceutical Bulletin

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Malignant diseases are responsible for the death of about one fifth of the population. The target of much research has been on the discovery of natural and synthetic compounds that can be used in the prevention and/or treatment of cancer. Many plants and animal extracts have shown various biological activities like immunopotentiating and antitumour activities. Honey bee propolis and its components (caffeic acid, caffeic acid phenethyl ester, artepilin C, quercetin, naringenin, resveratrol, galangin, genistein and other) are of the most promising as antitumour agent. [1][2][3][4][5][6][7][8] Propolis (bee glue) is the generic name for the resinous substance collected by honey bees from various plant sources and used by bees to seal holes in their honeycombs, smooth out the internal walls, and protect the entrance against intruders.1) It is rich in biochemical constituents, including mostly a mixture of polyphenols, flavonoid aglycones, phenolic acid and their esters, and phenolic aldehydes and ketones, terpenes, sterols, vitamins, amino acids, etc.2) Healing properties of propolis are known in folk medicine from antiquity. Recently, the interest for propolis as a harmless medicine is increasing. There have been many attempts to validate biological effects of propolis and elucidate its composition. [3][4][5][6][7][8] It was shown that propolis and its constituents have strong antimicrobial effect, acting on viruses, 9-11) bacteria, [12][13][14] and fungi. 15,16) It was also demonstrated that propolis and some of its active substances have a pronounced cytostatic, anticarcinogenic and antitumour effect both in "in vitro" and "in vivo" tumor models. [6][7][8][17][18][19][20][21][22] Immunomodulatory effects of propolis has also been recorded. [23][24][25][26] It has been suggested that the therapeutic activities of propolis depend mainly on the presence of flavonoids.28) Flavonoids have also been reported to induce the immune system, 29,30) and to act as strong oxygen radical scavengers. Dietary intake of antioxidant has been associated with a diminished risk of cancer at various anatomical sites. 31) This led us to compare how the routes (oral and/or systemic) of administration of different polyphenolic compounds deriving from propolis and of propolis itself influence on Ehrlich ascites tumor growth. Thus, we analyzed not only tumor growth but also parameters of immunomodulatory response of animals inoculated with test compounds, determining the effect of them on polymorphonuclear (PMN) and mononuclear (MN) cells, mainly macrophages. MATERIALS AND METHODSWater-Soluble Derivative of Propolis (WSDP) Treatment A water-soluble derivative of propolis (WSDP) was prepared by the method described elsewhere. 32) Briefly, Croatian propolis from beehives kept at the outskirts of Zagreb, Croatia or Brazilian propolis (CONAP, Belo Horizonte, Minas Gerais, Brazil) was extracted with 96% ethanol, which was filtered and evaporated to dryness in vacuum evaporator. The resultant resinous product was added to a stirred solution of 8%...

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Section: Count Of the Total Number Of Cells Present In The Peritonealmentioning

confidence: 99%

Synergystic Antitumor Effect of Polyphenolic Components of Water Soluble Derivative of Propolis against Ehrlich Ascites Tumour

Oršolić

Kosalec

Basić

2005

Biological & Pharmaceutical Bulletin

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“…Fenn demon strated that phagocytic events require the spreading of cell cytoplasm over the surface of particles. MNP undergo spreading in the presence of various inducers (Table 4) (23,57,178,193,195,196).…”

Section: Cy Toplasmic Spreadingmentioning

confidence: 99%

Dynamics of the Macrophage Plasma Membrance

Zuckerman¹,

Douglas²

1979

Annu. Rev. Microbiol.

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“…Polymorphonuclear leukocytes (PMN) and macrophages placed on a variety of surfaces rapidly adhere and spread themselves out thinly on the surface (1,2). This is thought to represent a frustrated attempt to phagocytose the surface (3).…”

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confidence: 99%

Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium.

Kruskal¹,

Shak²,

Maxfield³

1986

Proc. Natl. Acad. Sci. U.S.A.

106

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When human polymorphonuclear leukocytes (PMN) are placed on various surfaces, they attach and spread rapidly, increasing their diameter severalfold. The spreading is associated with extensive changes in the cytoskeleton. Since many cytoskeletal events are regulated by Ca2 , we measured the cytosolic free calcium concentration ([Ca2+] Polymorphonuclear leukocytes (PMN) and macrophages placed on a variety of surfaces rapidly adhere and spread themselves out thinly on the surface (1, 2). This is thought to represent a frustrated attempt to phagocytose the surface (3). This process is apparently distinct from adherence to endothelium, as PMN do not spread on an endothelial cell monolayer (4). During spreading, major changes in the organization of the actin-based cytoskeleton have been observed at the light-microscopic level (5, 6) and the ultrastructural level (1, 7). Boyles and Bainton (1) have observed the rapid formation of a network of thin filaments interconnected by globular centers only on the adherent surface of spreading PMN. In spreading macrophages, similar changes are seen (5, 7).Several major cytoskeletal proteins in PMN are regulated by calcium. Gelsolin, when activated by calcium concentrations greater than 100 nM, nucleates actin-filament growth, caps the fast-growing end of filaments, and splits preformed filaments (8). PMN also contain myosin, which, in muscle, is controlled by calcium in a well-characterized fashion (9).In order to measure cytosolic free calcium concentration ([Ca2+]j) in single cells, we developed a method using the calcium-sensitive fluorescent dye quin-2 and quantitative fluorescence microscopy (10). We have adapted that method to fura-2, a successor to quin-2 with improved fluorescence properties, such as an increased quantum yield and lower susceptibility to photobleaching (11

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Macrophage spreading in vitro

Cited by 144 publications

References 25 publications

Synergystic Antitumor Effect of Polyphenolic Components of Water Soluble Derivative of Propolis against Ehrlich Ascites Tumour

Synergystic Antitumor Effect of Polyphenolic Components of Water Soluble Derivative of Propolis against Ehrlich Ascites Tumour

Dynamics of the Macrophage Plasma Membrance

Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium.

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