Macrophage spreading in vitro

Rabinovitch, M.; DeStefano, Mary Jo

doi:10.1016/0014-4827(74)90629-6

Cited by 81 publications

(17 citation statements)

References 17 publications

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“…following paragraph) was added. In contrast, when PMN were incubated in the presence of maximal adhesion-promoting stimuli (250 nM f-Met-Leu-Phe, inulin-activated plasma), the cells lost their polarization, extended their hyaline lamellar processes over their entire circumference, and became highly flattened similar to spreaded monocytes (22). Although, these disk-like PMN were practically immobilized (checked by time-lapse cinematography), they showed continuous ruffling activity with extension and retraction of hyaline lamellae (Fig.…”

Section: Resultsmentioning

confidence: 97%

Modulating Influence of Chemotactic Factor-Induced Cell Adhesiveness on Granulocyte Function

Fehr¹,

Dahinden²

1979

J. Clin. Invest.

139

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A B S T R A C T The importance of adhesion in regulating locomotion and accumulation of polymorphonuclear leukocytes (PMN) has remained vague. We found that the chemotaxis of human PMN resuspended in heat-inactivated plasma was maximal toward 1-10 nM N-formyl-met-leu-phe (f-Met-Leu-Phe), but fell below random motility toward -100 nM. This impressive decrease of motility was paralleled by increased cell adherence on Petri dishes being minimal at 1 nM and maximal at >10 nM f-Met-Leu-Phe (6±1 and 37±2%[SE] adherent cells, respectively). Checked by phasecontrast microscopy, cells under stimulated adhesion lost the typical bipolar shape of moving PMN and became immobilized and highly flattened. PMN, preexposed to 250 nM f-Met-Leu-Phe and tested after washing, retained increased adhesiveness and showed extremely low random and chemotactic motility. In contrast, preexposure to 1 nM f-Met-Leu-Phe had no effect on chemotaxis. Supporting the concept that immobilizing hyperadhesiveness does not correspond to a general functional hyporesponsiveness of PMN, no depression of the initial ingestion rate was observed in the presence of 250 nM f-Met-Leu-Phe. Moreover, a close correlation was found between the induction of PMN adhesiveness and the stimulation of the hexose monophosphate pathway activity as well as of lysomal enzyme release (r -0.98). Thus, "chemotactic deactivation" and "high-dose inhibition of chemotaxis" by N-formyl peptides is the consequence of increased cell adhesiveness. This phenomenon provides a mechanism for cell trapping at the inflammatory site. Conversely, if operative in circulating blood, e.g., in septicemia, it may impair PMN emigration to such sites.This work was presented in part at a multidisciplinary ses-

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Section: Resultsmentioning

confidence: 97%

Modulating Influence of Chemotactic Factor-Induced Cell Adhesiveness on Granulocyte Function

Fehr¹,

Dahinden²

1979

J. Clin. Invest.

139

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“…The mode of attachment and spreading of these cells is generally influenced to a great extent by various factors such as the type of cells, their state of maturation, age and species of the animal from which the cells are obtained, constituents of the culture medium, particularly the presence or absence of certain serum proteins, the nature of the surface of the substrate, the length of incubation time, and the cell density (6,9,12,13,19). Under certain defined conditions, however, the enhancement of macrophage attachment and spreading, particularly that of spreading, has been considered to reflect macrophage activation (1,11,13). We confirmed this in the present study, using lipopolysaccharide and lymphokines known to be macrophage stimulants.…”

Section: Discussionmentioning

confidence: 99%

Macrophage Activation by Muramyl Dipeptide as Measured by Macrophage Spreading and Attachment

Tanaka

Nagao

Imai

et al. 1980

Microbiology and Immunology

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A synthetic bacterial cell wall constituent, muramyl dipeptide (MDP), was found to induce the enhancement of macrophage spreading and attachment on glass or plastic surfaces. Macrophages exposed to bacterial lipopolysaccharide or lymphokine-containing cell supernatants showed similar enhancement. This finding supports the view that MDP activates macrophages. MDP was also found to enhance the viability of macrophages but to inhibit 3H-thymidine incorporation by macrophages.

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“…The present results indicate, however, that these drugs also influence membrane organization via an interaction with membrane-associated MT and MF. The inhibitory effect of local anesthetics on the adhesion, spreading and locomotion of cells on solid substrates (42,43), cell aggregation (44), axonal transport (45), endocytosis (46), and exocytosis (47), might now be reevaluated as possibly resulting from the action of these drugs on cytoskeletal elements rather than their single action on membrane lipids particularly since these various processes are also inhibited by drugs acting on MT and MF systems (48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

Local anesthetics affect transmembrane cytoskeletal control of mobility and distribution of cell surface receptors.

Poste¹,

Papahadjopoulos²,

Nicolson³

1975

Proc. Natl. Acad. Sci. U.S.A.

162

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Tertiary amine local anesthetics facilitated concanavalin A-induced redistribution of lectin receptors on murine BALB/3T3 cells and enhanced the susceptibility of these cells to agglutination by concanavalin A. In contrast, these drugs at similar concentrations inhibited ligand-induced capping of immunoglobulin receptors on mouse lymphocytes. We propose that these differing effects of local anesthetics on membrane receptor mobility in fibroblasts and lymphocytes result from the action of anesthetics on membrane-associated microtubules and microfilaments involved in the transmembrane control of receptor mobility. We present electron microscopic evidence of structural alterations in microtubule and microfilament organization in anesthetic-treated cells, together with data on changes in the responsiveness of anesthetic-treated cells to drugs that act on microtubules and/or microfilaments. This evidence supports the proposal that anesthetics affect the organization of cytoskeletal components or their plasma membrane attachment points. The effects of local anesthetics on ligand-induced redistribution of membrane receptors in both 3T3 cells and lymphocytes can be duplicated by treating cells with colchicine (or Vinca alkaloids) together with cytochalasin B. We propose that the participation of membrane-associated microtubules and microfilaments in the transmembrane control of receptor mobility is such that microtubules and microfilaments play opposing roles in regulating the mobility and topograpy of cell surface receptors.Binding of multivalent ligands such as lectins or antibodies to the cell surface is known to be accompanied by changes in the topographical distribution of the appropriate receptors (1, 2). Ligand-induced changes in receptor distribution offer a potential mechanism whereby events occurring on the outer surface of the plasma membrane could be translated to the cytoplasmic side and this might provide a structural basis for transducing information between the external environment and the intracellular metabolic machinery in such phenomena as mitogenesis, cell recognition and communication, and cellular immune responses.The mobility and distribution of certain lectin and immunoglobulin receptors on the surfaces of mammalian cells may be under transmembrane control by cytoplasmic structural elements associated with the plasma membrane that are sensitive to microtubule (MT) disruptive drugs such as colchicine (Ch) and the Vinca alkaloids (2-8). These membrane-associated proteins may act as "anchors" to restrict receptor mobility. Dislocation of the linkage between surface receptors and the Ch-sensitive structures allows increased receptor mobility and facilitates receptor redistribution by external multivalent ligands (2-8). The mobility and distribution of lectin and immunoglobulin receptors is also influenced by cytochalasin B (CB), a drug that disrupts microAbbreviations: CB, cytochalasin B; Ch, colchicine; Con A, concanavalin A; MF, microfilaments; MT, microtubules. filaments (MF) (2, 9-11). ...

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Macrophage spreading in vitro

Cited by 81 publications

References 17 publications

Modulating Influence of Chemotactic Factor-Induced Cell Adhesiveness on Granulocyte Function

Modulating Influence of Chemotactic Factor-Induced Cell Adhesiveness on Granulocyte Function

Macrophage Activation by Muramyl Dipeptide as Measured by Macrophage Spreading and Attachment

Local anesthetics affect transmembrane cytoskeletal control of mobility and distribution of cell surface receptors.

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