Macrophage/T‐Lymphocyte Interaction in the Immune Response to PPD in Humans

Abstract

The proliferative response of sensitized human T cells to low concentrations of purified protein derivative of tuberculin (PPD) in vitro has previously been shown to depend on the presence of autologous or HLA-D-compatible macrophages (M phi). In this paper we present evidence that the M phi/T cell interaction requires viable M phi and does not involve a soluble factor. T cells from neonates do not respond to PPD in our system, while M phi from neonates are able to present PPD to T cells from HLA-D matched sensitized adults. It is also demonstrated that the previously reported HLA-D restriction of T cell/M phi cooperation is not due to T cell mediated killing of allogeneic macrophages in vitro.

Evidence in support of a self-perpetuating HLA-DR-dependent delayed-type cell reaction in rheumatoid arthritis.

“…4). Of the two tested antigens, PPD represents a well-characterized positive control antigen that is meant to define our experimental T-cell system (35). Cartilage-derived collagen type II, on the other hand, is currently studied with great interest as a possible autoimmunogen of importance in rheumatoid arthritis (37,38).…”

Analysis of in situ Inflammation of Allogeneic Canine Kidney Grafts under Antilymphocyte Globulin Treatment

“…It is known that monocytes in fluence the afferent and efferent Limb of the immune response: the antigen presentation depends on T cell activation [3,5,6,15]. We, therefore, suggest that due to the lack of anti gen-responding lymphocytes under ALG treatment, the less ALG-sensitive monocytes increase in the graft during rejection epi sodes.…”

“…We, therefore, suggest that due to the lack of anti gen-responding lymphocytes under ALG treatment, the less ALG-sensitive monocytes increase in the graft during rejection epi sodes. In addition it has been reported [3,16] that high concentrations of monocytes result in diminished immune response. The opti mal activation of T lymphocytes seems to be reached at day 7.…”

Antigen presentation and regulatory functions of human monocytes in the in vitro response of lymphocytes against purified protein derivative of tuberculin (PPD)

“…Lymphoproliferative response in vitro are also regulated by M a [14, 161 and may be initiated by antigen-fed M a [3,[16][17][18][19][20]. Although most investigators suggest that either MQ [3, 4, 17, 211 or their soluble products [22, 231 are essential for this response, other investigators have shown that T lymphocyte proliferation may also be induced independently of MQ [20, 22, 241. In human systems, it is as yet unclear which of the MQ functions is an essential part of the lymphoproliferative response against soluble antigen.…”