Macrophages/microglial cells in human central nervous system during development: an immunohistochemical study

Macrophage and microglia ontogeny in the mouse visual system can be traced by the expression of Cathepsins B and D

“…Microglial cells are derived from the monocyte/macrophage lineage, and they progressively enter the nervous system during development (Cuadros and Navascues, 1998). Nondifferentiated microglial cells thus label with typical macrophage markers, such as lectins (Acarin et al, 1994;Andjelkovic et al, 1998) or different phagocyte antibodies (Kullberg et al, 2001;Choi et al, 2004;Herber et al, 2006). In agreement with our results, in most of these cases the intensity of the staining with different markers greatly diminishes as the microglia ramify.…”

CD163 Identifies Perivascular Macrophages in Normal and Viral Encephalitic Brains and Potential Precursors to Perivascular Macrophages in Blood

“…66 -69 In contrast, perivascular macrophages appear later when the vasculature develops. 67,70 Previous studies in adult rodents have shown that macrophages in the perivascular, meningeal, and choroid plexus turnover regularly, whereas the repopulation of microglia by bone marrow precursors is limited, at least under normal conditions. 3,[71][72][73] Therefore, perivascular macrophages in adult and developing CNS are undoubtedly of monocytic origin.…”

Perspectives of Stem Cell-Derived Microglia for Medicine

“…Infiltration of monocytes could be confirmed by carbon-labelling (Ling et al, 1980) and histochemical studies using marker enzymes for monocytes (Ferrer and Sarmiento, 1980;Ling et al, 1982). Andjelkovic and colleagues labelled microglia in the developing and adult human brain using lectin-histochemistry (Andjelkovic et al, 1998). Lectin positive cells can be subdivided into two groups which both exist in the adult brain, but show different distribution and time schedule of morphological changes.…”