Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

Plas, Mariena J. A. van der; Jukema, G. N.; Wai, Sin Wen; Dogterom-Ballering, Heleen C.M.; Lagendijk, E.; Gulpen, Co van; Dissel, Jaap T. van; Bloemberg, Guido V.; Nibbering, Peter H.

doi:10.1093/jac/dkm407

Cited by 157 publications

(106 citation statements)

References 37 publications

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“…van der Plas et al also reported biofilm reduction at concentrations up to 20 lg ES per well [22]. In our in vitro experiments, each maggot produced more than 1.8 lg ES per hour.…”

Section: Discussionsupporting

confidence: 67%

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The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

Cazander

Veen

Bouwman

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Biofilm formation in wounds and on biomaterials is increasingly recognized as a problem. It therefore is important to focus on new strategies for eradicating severe biofilm-associated infections. The beneficial effects of maggots (Lucilia sericata) in wounds have been known for centuries. We hypothesized sterile maggot excretions and secretions (ES) could prevent, inhibit, and break down biofilms of Pseudomonas aeruginosa (PAO1) on different biomaterials. Therefore, we investigated biofilm formation on polyethylene, titanium, and stainless steel. Furthermore, we compared the biofilm reduction capacity of Instar-1 and Instar-3 maggot ES and tested the temperature tolerance of ES. After biofilms formed in M63 nutrient medium on comb-forming models of the biomaterials, ES solutions in phosphate-buffered saline or M63 were added in different concentrations. PAO1 biofilms adhered tightly to polyethylene and titanium but weakly to stainless steel. Maggot ES prevent and inhibit PAO1 biofilm formation and even break down existing biofilms. ES still had considerable biofilm reduction properties after storage at room temperature for 1 month. ES from Instar-3 maggots were more effective than ES from Instar-1 maggots. These results may be relevant to patient care as biofilms complicate the treatment of infections associated with orthopaedic implants.

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“…van der Plas et al also reported biofilm reduction at concentrations up to 20 lg ES per well [22]. In our in vitro experiments, each maggot produced more than 1.8 lg ES per hour.…”

Section: Discussionsupporting

confidence: 67%

“…However, they found inhibition in the turbidimetric assay and concluded the antibacterial activity depends on the kind of experiment. The first report that described the possible activity of ES against biofilms of P. aeruginosa and S. aureus in in vitro experiments is the recently published paper by van der Plas et al [22].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

Cazander

Veen

Bouwman

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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“…Valachova et al (2014) identified the full-length cDNAs of five novel putative salivary proteases of L. sericata, three of them from the serine protease families which could play a significant role in debridement of wounds. Also, recent studies investigated the potentials of sterile L. sericata ES to prevent the formation and disrupt bacterial biofilms of S. aureus and P. aeruginosa (van der Plas et al, 2008). It was found that ES prevented and disrupted S. aureus biofilms immediately and enhanced the formation of P. aeruginosa biofilms for 10 h after incubation, and then it began to breakdown P. aeruginosa biofilms (van der Plas et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Maggot excretions/secretions (ES) antimicrobial components have been shown to disinfect wounds by destroying bacteria that often do not respond to commercially available antibiotics, such as methicillinresistant S. aureus (MRSA) (Bexfield et al, 2004). Furthermore, maggot ES inhibited the pro-inflammatory response of human neutrophils and monocytes in the wound healing process (van der Plas et al, 2007(van der Plas et al, , 2009a. These secretions contained factors that could break down and inhibit S. aureus and P. aeruginosa biofilms, which colonized wounds and protected harmful bacteria from both the host immune system and therapeutic antibiotics.…”

Section: Introductionmentioning

confidence: 99%

In vitro antimicrobial activity of maggot excretions/secretions of Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy)

El-Bassiony¹,

Stoffolano²

2016

Afr. J. Microbiol. Res.

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The excessive usage of conventional antibiotics leads to the emergence of multidrug-resistant bacterial strains which threaten public health and stimulates searching for new sources of bio-therapeutic drugs. The aim of this study was to investigate the antimicrobial activity of maggot excretions/secretions from larvae of Sarcophaga argyrostoma, a common species of the family Sarcophagidae in Egypt. The excretions/secretions (ES) produced by third instar larvae were sterile filtered and tested against selected pathogenic strains of Gram positive (Gram+ve) bacteria, Staphylococcus aureus and Bacillus subtilis; Gram negative (Gram-ve) bacteria, Escherichia coli and Pseudomonous aeruginosa; and the filamentous fungus Aspergillus flavus. The ES product produced by third instar maggots proved to be more effective against Gram-ve bacteria. Larval ES, at 0.125 mg/ml concentration, were significantly potent towards P. aeruginosa, E. coli and S. aureus in a descending sequence. The minimum inhibitory concentrations of S. argyrostoma ES were 0.125 mg/ml for P. aeruginosa and E. coli, using the turbidimetric assay method. Twice and four times this concentration were required to inhibit growth of S. aureus (0.25 mg/ml) and B. subtilis (0.5 mg/ml), respectively. The antibacterial properties of S. argyrostoma ES were not affected by heating or freeze-thaw cycles when tested against E. coli.

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“…A number of studies have demonstrated that bacteria, such as Escherichia coli are consumed and then eradicated in the acidic midgut of L. sericata larvae (Robinson and Norwood, 1934;Greenberg, 1968;Mumcuoglu et al, 2001;Daeschlein et al, 2007) and that the externalised larval excretion/secretion (ES) can reduce the viability of a number of Gram-negative and Gram-positive organisms (Simmons, 1935;Thomas et al, 1999;Bexfield et al, 2004;Kerridge et al, 2005;Bexfield et al, 2008;Jaklic et al, 2008;van der Plas et al, 2008;Cazander et al, 2009a;Harris et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Barnes

Dixon

Gennard

2010

Journal of Microbiological Methods

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Research to quantify the potency of larval excretion/secretion from Lucilia sericata using liquid culture assays has produced contradictory results. In this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and the effects of varying growing conditions in assays. Results demonstrate that factors such as number of larvae, species of bacteria and addition of nutrient influence its antibacterial potency. Therefore a standardised method should be employed for liquid culture assays when investigating the antibacterial activity of larval excretion/secretion from L. sericata

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Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

Abstract: Maggot ES are differentially effective against biofilms of S. aureus and P. aeruginosa.

Cited by 157 publications

References 37 publications

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

In vitro antimicrobial activity of maggot excretions/secretions of Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy)

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

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