Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model

Li, Zheng; Wang, Jia Li; Xu, Jian; Zhang, Xiao Tian; Liu, Bao Yi; Huang, Le; Zhang, Ri; Zu, Hai Yue; He, Xuan; Mi, Jie; Pang, Qian; Wang, Xin Luan; Ruan, Ye Chun; Zhao, Dewei; Qin, Ling

doi:10.1016/j.biomaterials.2020.119828

Cited by 79 publications

(41 citation statements)

References 89 publications

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“…Niflumic acid, a Cl − channel antagonist, does not alter Mg 2+ currents, indicating that extracellular Cl − has no influence on the transport of Mg 2+ [ 73 ]. Besides, both in vitro and in vivo results demonstrate that the high mineralization is realized with a concentration of 0.8 mM Mg 2+ , which is consistent with previous study [ 37 ].…”

Section: Discussionsupporting

confidence: 92%

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Synergistic effects of magnesium ions and simvastatin on attenuation of high-fat diet-induced bone loss

Dai

et al. 2021

Bioactive Materials

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Introduction Magnesium (Mg) has a prophylactic potential against the onset of hyperlipidemia. Similar to statin, Mg is recommended as lipid-lowering medication for hypercholesterolemia and concomitantly exhibits an association with increased bone mass. The combination of statin with Mg ions (Mg 2+ ) may be able to alleviate the high-fat diet (HFD)-induced bone loss and reduce the side-effects of statin. This study aimed to explore the feasibility of combined Mg 2+ with simvastatin (SIM) for treating HFD-induced bone loss in mice and the involving mechanisms. Materials and methods C57BL/6 male mice were fed with a HFD or a normal-fat diet (NFD). Mice were intraperitoneally injected SIM and/or orally received water with additional Mg 2+ until sacrificed. Enzyme-linked immunosorbent assay was performed to measure cytokines and cholesterol in serum and liver lysates. Bone mineral density (BMD) and microarchitecture were assessed by micro-computed tomography (μCT) in different groups. The adipogenesis in palmitate pre-treated HepG2 cells was performed under various treatments. Results μCT analysis showed that the trabecular bone mass was significantly lower in the HFD-fed group than that in NFD-fed group since week 8. The cortical thickness in HFD-fed group had a significant decrease at week 24, as compared with NFD-fed group. The combination of Mg 2+ and SIM significantly attenuated the trabecular bone loss in HFD-fed mice via arresting the osteoclast formation and bone resorption. Besides, such combination also reduced the hepatocytic synthesis of cholesterol and inhibited matrix metallopeptidase 13 ( Mmp13 ) mRNA expression in pre-osteoclasts. Conclusions The combination of Mg 2+ and SIM shows a synergistic effect on attenuating the HFD-induced bone loss. Our current formulation may be a cost-effective alternative treatment to be indicated for obesity-related bone loss.

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Section: Discussionsupporting

confidence: 92%

“…After fixation in 4% paraformaldehyde (PFA) for 24 h, bone tissues were decalcified in 10% ethylene diamine tetraacetic acid (EDTA, pH 7.4, Sigma, cat# 798681) for 14 days, embedded in paraffin to prepare sections (5 μm thick) for routine staining [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of magnesium ions and simvastatin on attenuation of high-fat diet-induced bone loss

Dai

et al. 2021

Bioactive Materials

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“…Zheng LZ et al. [ 27 ] showed a good outcome using steroid-associated osteonecrosis model of rat. Traumatic ONFH was also common in clinical and the model of ONFH has the advantages of being fast and stable, so we ameliorated a rat model of traumatic ONFH to evaluate the efficacy of zoledronic acid treatment.…”

Section: Discussionmentioning

confidence: 99%

Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

Zhao

Tian

et al. 2021

Journal of Orthopaedic Translation

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Background Osteonecrosis of the femoral head (ONFH) is a refractory disease due to its unclear pathomechanism. Neither conservative treatment nor surgical treatment during the early stage of ONFH achieves satisfactory results. Therefore, this study aims to explore the available evidence on the effect of zoledronic acid on early-stage ONFH. Methods For groups were established:the Normal group, model group, Normal saline group(NS group) and zoledronic acid-treated group . The blood supply to the femoral head of animals in the model group and zoledronic acid-treated group was interrupted via a surgical procedure, and zoledronic acid was then locally administered to the femoral head. Four weeks after surgery, all the hips were harvested and evaluated by micro-CT and histopathology(H&E staining, TRAP staining, Toluidine blue staining and masson staining). Results The values of BMD, BS/BV and Tb.Th in the Normal group and zoledronic acid-treated group were significantly higher than those in the model group and NS group (p < 0.05). The outcome of H&E staining, Toluidine blue staining and masson staining were consistent with that of micro-CT. Conclusion The local administration of zoledronic acid in the femoral head had positive effects on the bone structure of the femoral head in a modified rat model of traumatic ONFH and offered a promising therapeutic strategy during the early stage of ONFH. The Translational potential of this article This article could provide a choice for treating patients who have osteonecrosis of femora head and can be the basic research for advanced development over this disease

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“…9 Their results also revealed that vitamin C supplementation led to improved bone mineral density (BMD) and bone volume, a higher number of osteoblasts and osteoclasts, and increased expression of osteoblast differentiation genes in OVX rats. 9 Similarly, Zheng et al 33 Recent human studies have examined the link between vitamin C and fracture risk. [36][37][38] A study by Sun et al 36 evaluated the connection between antioxidant intake and risk of hip fracture in the elderly Chinese.…”

Section: Bone Healing and Osteoporosismentioning

confidence: 99%

Vitamin C in orthopedic practices: Current concepts, novel ideas, and future perspectives

Oakes

Bolia

Weber

et al. 2021

Journal Orthopaedic Research

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Vitamin C (ascorbic acid), is an important antioxidant that has been applied broadly in the field of orthopaedics. Current research on vitamin C examines the molecule's role in bone and tendon physiology, as well as joint replacement and Postoperative pain. Most laboratory and human studies associate the use of vitamin C with improved bone health and tendon healing. Recent literature moderately supports the use of vitamin C to improve functional outcomes, decreased postoperative pain, and prevent complex regional pain syndrome following orthopaedic procedures. The perioperative use of vitamin C in patients undergoing joint replacement surgery and anterior cruciate ligament reconstruction is still under investigation. Overall, there is need for high-quality human trials to confirm whether vitamin C can potentiate the outcomes of orthopaedic procedures and to determine optimal dosage and means of administration to maximize its proposed benefits. The purpose of this review was to summarize the application of vitamin C in orthopaedic practices and to identify potential areas for future study. K E Y W O R D S bone, orthopedics, pain management, tendons, vitamin C 1 | INTRODUCTION Vitamin C (ascorbic acid), is an important antioxidant and deficiency of this mineral is most often associated with scurvy. 1 Vitamin C is vital to the production of collagen in both bone and connective tissue and it has been associated with improved collagen synthesis and subsequent tendon healing. 2-4 It also neutralizes free radicals, which helps to decrease oxidative stress and inflammation. 5 Vitamin C has also been used as a supplement in orthopaedic patients. 6-8 Vitamin C has been linked to improved bone mineral density as well as decreased risk of bone fracture and osteoporosis. 9-11 Furthermore, there is recent evidence to suggest that vitamin C may have a protective role in osteoarthritis. 12 From a postoperative recovery perspective, vitamin C has been found to reduce the incidence of complex regional pain syndrome (CRPS) and improve functional outcomes. 5,13 However, some of these conclusions on the therapeutic effect of vitamin C continue to be debated. 14 The purpose of this review was to summarize the role of vitamin C in orthopaedic practices and to identify potential areas for future study. 1.1 | Perioperative pain management Postoperative pain exists as a common challenge to orthopaedic surgeons, as it is linked to increased risk of postoperative complications, longer hospital stays, and significant delays in the return to regular function. 15 Opioids, such as morphine, may be prescribed to limit postoperative pain in orthopedic patients; however, there are a multitude of side effects associated with their use

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Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model

Cited by 79 publications

References 89 publications

Synergistic effects of magnesium ions and simvastatin on attenuation of high-fat diet-induced bone loss

Synergistic effects of magnesium ions and simvastatin on attenuation of high-fat diet-induced bone loss

Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

Vitamin C in orthopedic practices: Current concepts, novel ideas, and future perspectives

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