Magnesium deficiency-induced changes in organic acid metabolism of Citrus sinensis roots and leaves

Yang, Lin-Tong; Yang, Gang-Hua; You, Xiang; Zhou, Chuifan; Lu, Yi-Bin; Chen, Li‐Song

doi:10.1007/s10535-013-0313-5

Cited by 24 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…O fato do N reduzir a massa da raiz já era esperado, pois esse elemento é requerido em grande quantidade pelas culturas agrícolas e é o mais limitante para o crescimento e desenvolvimento das plantas (OLIVEIRA et al, 2010). O Mg tem como função ativar ou reguladar várias quinases, ATPases, carboxilase/oxigenase RuBP e outras enzimas do metabolismo de carboidratos (MARSCHNER, 2012) e dessa forma, a falta deste nutriente pode causar a redução do crescimento das raízes (YANG et al, 2013;CAKMAK, 2013).…”

Section: Resultsunclassified

Deficiência De Nutrientes Com Efeitos No Desenvolvimento De Morangueiros

Barreto

Silva

Navroski

et al. 2017

RSA

View full text Add to dashboard Cite

A adubação do morangueiro é uma das principais práticas responsáveis pelo aumento da produção. Entretanto, ainda são insuficientes as informações sobre o efeito da omissão dos nutrientes minerais sobre o desenvolvimento do morangueiro. Neste contexto, o objetivo deste trabalho foi avaliar o crescimento dos morangueiros, visualizar os sintomas de deficiência nutricionais e os teores de nutrientes nas folhas decorrentes da omissão de macro e micro nutrientes. O delineamento experimental utilizado foi o inteiramente casualizado, com dez tratamentos e três repetições. Os tratamentos constituíram-se de solução completa e omissão individual de cada um dos seguintes nutrientes: nitrogênio (N), fósforo (P), potássio (K), cálcio (Ca), magnésio (Mg), cobre (Cu), manganês (Mn), zinco (Zn) e ferro (Fe). Avaliou-se: a massa fresca e seca da raiz, da parte aérea e da coroa, número de folhas e coroas, teores de macro e micronutrientes nas folhas e sintomas visuais de deficiência nutricional. A massa fresca e seca da parte aérea, o número de coroa e o número de folhas são mais prejudicados pela ausência de macronutrientes. O desenvolvimento do sistema radicular do morangueiro é menor na omissão dos macronutrientes. Menores teores de N, P, K, Ca, Mg e Fe nas folhas de morangueiro ocorrem quando são realizadas omissão desses elementos

show abstract

Section: Resultsunclassified

Deficiência De Nutrientes Com Efeitos No Desenvolvimento De Morangueiros

Barreto

Silva

Navroski

et al. 2017

RSA

View full text Add to dashboard Cite

show abstract

“…In addition, Mg also act as a cofactor and allosteric modulator for more than 300 enzymes including ribulose-1,5-bisphosphate carboxylase (Rubisco), ATPase, protein kinases, RNA polymerase, phosphatases and glutathione synthase [ 1 , 4 ]. Therefore, Mg also functions in many other physiological processes, such as respiration [ 7 ], glycolysis, tricarboxylic acid (TCA) cycle [ 8 ], energy transfer via adenosine triphosphate [ 9 ], carbohydrate partitioning between source and sink organs [ 1 , 10 ], reactive oxygen species (ROS) formation and related photooxidative damage [ 5 , 11 ], protein biosynthesis, and the formation of DNA and RNA [ 3 ]. Accordingly, a number of studies have investigated the effects of Mg-deficiency on Chl synthesis, transport and utilization of photosynthates [ 1 ], photochemical reactions, CO 2 fixation [ 2 , 4 - 6 ], respiration, TCA cycle [ 7 , 8 ], and ROS metabolism [ 4 , 5 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

View full text Add to dashboard Cite

BackgroundMagnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM MgSO4) or Mg-sufficient (1 mM MgSO4) nutrient solution for 16 weeks. Thereafter, we first investigated the proteomic responses of C. sinensis roots and leaves to Mg-deficiency using two-dimensional electrophoresis (2-DE) in order to (a) enrich our understanding of the molecular mechanisms of plants to deal with Mg-deficiency and (b) understand the molecular mechanisms by which Mg-deficiency lead to a decrease in photosynthesis.ResultsFifty-nine upregulated and 31 downregulated protein spots were isolated in Mg-deficient leaves, while only 19 upregulated and 12 downregulated protein spots in Mg-deficient roots. Many Mg-deficiency-responsive proteins were involved in carbohydrate and energy metabolism, followed by protein metabolism, stress responses, nucleic acid metabolism, cell wall and cytoskeleton metabolism, lipid metabolism and cell transport. The larger changes in leaf proteome versus root one in response to Mg-deficiency was further supported by our observation that total soluble protein concentration was decreased by Mg-deficiency in leaves, but unaffected in roots. Mg-deficiency had decreased levels of proteins [i.e. ribulose-1,5-bisphosphate carboxylase (Rubisco), rubisco activase, oxygen evolving enhancer protein 1, photosynthetic electron transfer-like protein, ferredoxin-NADP reductase (FNR), aldolase] involved in photosynthesis, thus decreasing leaf photosynthesis. To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism.ConclusionsOur results demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long-term Mg-deficiency. Mg-deficiency decreased the levels of many proteins involved in photosynthesis, thus decreasing leaf photosynthesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1462-z) contains supplementary material, which is available to authorized users.

show abstract

“…As primary metabolites, organic acids not only contribute to fruit quality (Ladaniya ), but also enable plants to respond to abiotic stress (Shlizerman et al , Chaffai and Marzouk , Li et al , Yang et al , , Liao et al ). Although some characteristics of organic acid accumulation in fruits are commonly accepted, the underlying mechanisms are distinct in different fruits.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)

XiongJun

et al. 2016

Physiologia Plantarum

View full text Add to dashboard Cite

Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.

show abstract

Magnesium deficiency-induced changes in organic acid metabolism of Citrus sinensis roots and leaves

Cited by 24 publications

References 36 publications

Deficiência De Nutrientes Com Efeitos No Desenvolvimento De Morangueiros

Deficiência De Nutrientes Com Efeitos No Desenvolvimento De Morangueiros

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)

Contact Info

Product

Resources

About