2012
DOI: 10.4269/ajtmh.2012.11-0716
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Magnetic Affinity Enzyme-Linked Immunoassay for Diagnosis of Schistosomiasis Japonicum in Persons with Low-Intensity Infection

Abstract: Abstract. Most schistosome-endemic areas in China are characterized by low-intensity infections that are independent of prevalence. To establish an effective diagnostic method, we developed a magnetic affinity enzyme-linked immunoassay based on soluble egg antigens (SEA-MEIA) for diagnosing schistosomiasis in persons with low-intensity infection with Schistosoma japonicum by comparing it with a conventional enzyme-linked immunosorbent assay (ELISA). Our results showed that the SEA-MEIA had a higher sensitivity… Show more

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Cited by 7 publications
(2 citation statements)
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“…Recently, more rapid, sensitive and portable diagnostic assays to detect human antibodies against S. japonicum, have been developed and tested in areas of low endemicity for schistosomiasis japonica in China (Table 3). These methods include: (1) DDIA (Xu et al 2011a) which is commercially available in China, and the dipstick with latex immunochromatographic assay (DLIA) (Yu et al 2011); (2) magnetic affinity enzyme-linked immunoassays (MEIAs), based on the signal transduction protein 14-3-3 (Sj14-3-3), recombinant 26 kDa GST (rSj26GST) (Yu et al 2012a), or SEA-MEIA (Yu et al 2012b);…”
Section: Immunological Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Recently, more rapid, sensitive and portable diagnostic assays to detect human antibodies against S. japonicum, have been developed and tested in areas of low endemicity for schistosomiasis japonica in China (Table 3). These methods include: (1) DDIA (Xu et al 2011a) which is commercially available in China, and the dipstick with latex immunochromatographic assay (DLIA) (Yu et al 2011); (2) magnetic affinity enzyme-linked immunoassays (MEIAs), based on the signal transduction protein 14-3-3 (Sj14-3-3), recombinant 26 kDa GST (rSj26GST) (Yu et al 2012a), or SEA-MEIA (Yu et al 2012b);…”
Section: Immunological Methodsmentioning
confidence: 99%
“…Recently, more rapid, sensitive and portable diagnostic assays to detect human antibodies against S. japonicum , have been developed and tested in areas of low endemicity for schistosomiasis japonica in China (Table 3). These methods include: (1) DDIA (Xu et al 2011 a ) which is commercially available in China, and the dipstick with latex immunochromatographic assay (DLIA) (Yu et al 2011); (2) magnetic affinity enzyme-linked immunoassays (MEIAs), based on the signal transduction protein 14-3-3 (Sj14-3-3), recombinant 26 kDa GST (rSj26GST) (Yu et al 2012 a ), or SEA-MEIA (Yu et al 2012 b ); (3) a time-resolved fluoroimmunoassay (TRFIA), established for detecting Sj14-3-3, as a circulating antigen in serum (Qian et al 2011); (4) an electrochemical immunosensor array (ECISA) assay (Deng et al 2013) which uses a recombinant S. japonicum calcium-binding protein (SjE16), with SEA, co-immobilized on a disposable 16-channel screen-printed carbon electrode array. Antibodies in serum samples can be detected by a portable electrochemical detector; (5) a multiplexed bioanalytical assay which is developed by fusing 2 types of gold nanorods (GNRs) (Huang et al 2012).…”
Section: Development and Testing Of New Diagnostics For Schistosomiasismentioning
confidence: 99%