Magnetic Affinity Microspheres with Meso-/Macroporous Shells for Selective Enrichment and Fast Separation of Phosphorylated Biomolecules

Abstract

The flowerlike multifunctional affinity microspheres prepared by a facile solvothermal synthesis and subsequent calcination process consist of magnetic cores and hierarchical meso-/macroporous TiO2 shells. The hierarchical porous structure of the flowerlike affinity microspheres is constructed by the macroporous shell from the stacked mesoporous nanopetals which are assembled by small crystallites. The affinity microspheres have a relatively large specific surface area of 50.45 m(2) g(-1) and superparamagnetism with a saturation magnetization (Ms) value of 30.1 emu g(-1). We further demonstrate that they can be applied for rapid and effective purification of phosphoproteins, in virtue of their selective affinity, porous structure, and strong magnetism. In addition, the affinity microspheres can also be used for enrichment of phosphopeptides, and the selectivity is greatly improved due to the increase of mass transport and prevention of the possible "shadow effect" resulting from the smaller and deeper pores by taking advantage of the unique porous structure. Overall, this work will be highly beneficial for future applications in the isolation and identification of phosphorylated biomolecules.

β-cyclodextrin functionalized meso-/macroporous magnetic titanium dioxide adsorbent as extraction material combined with gas chromatography-mass spectrometry for the detection of chlorobenzenes in soil samples

“…After coating of meso-/macroporous TiO 2 shells, the obtained microspheres showed a flower-like porous surface (Fig. 2b), in agreement with previous findings [30]. The overall thickness of the TiO 2 shell was about 30 nm ( Fig.…”

“…The characteristic adsorption band of Fe-O was found at 568 cm −1 (Fig. 4a) [24] and [30]. The stretching vibration of Ti O bonds of the TiO 2 shell was represented at 465 cm −1 (Fig.…”

“…The magnetic properties were analyzed through a vibrating sample magnetometer (VSM, PPMS-9), which was purchased from Quantum Design, Ltd., USA. The magnetic Fe 3 O 4 particles were prepared according to the previous reported method [30]. The flowerlike Fe 3 O 4 @fTiO 2 hierarchical microspheres were prepared by a facile solvothermal method [30].…”

“…The magnetic Fe 3 O 4 particles were prepared according to the previous reported method [30]. The flowerlike Fe 3 O 4 @fTiO 2 hierarchical microspheres were prepared by a facile solvothermal method [30]. Typically, the as synthesized Fe 3 O 4 particles (160 mg) were fully dispersed in DMF (40 mL) and isopropanol (120 mL) under ultrasonication.…”

Novel molecularly imprinted stir bar sorptive extraction based on an 8-electrode array for preconcentration of trace exogenous estrogens in meat

“…Interestingly, Cheng et al [16] developed a novel magnetic composite microsphere with hierarchical meso-/macroporous TiO 2 shells using a facile solvothermal method. The microspheres can be employed to effectively enrich phospho-proteins and phospho-peptides by taking advantage of the meso-/macroporous affinity structure, which can increase mass transport of targets.…”