Magnetic-assisted aptamer-based fluorescent assay for allergen detection in food matrix

Zhang, Youxiong; Wu, Qingping; Sun, Ming; Zhang, Jumei; Mo, Shuping; Wang, Juan; Wei, Xianhu; Bai, Jianling

doi:10.1016/j.snb.2018.02.098

Cited by 54 publications

(21 citation statements)

References 35 publications

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“…Under the condition of high concentration, the fluorescence intensity weakened rapidly. The reason may be that the high concentration of Fe 3 O 4 @Ab1 IgG caused the aggregation phenomenon 35 − 37 or the color of Fe 3 O 4 @Ab1 IgG (black) interfered with the fluorescence of the immunocomplex. 38 , 39 Similarly, the phenomenon of fluorescence weakening caused by excessive concentration of signal detection probe is consistent with other reports.…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres

et al. 2020

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The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination of human IgG and then expand it to analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM and IgG, which is of great significance for the screening and diagnosis of COVID-19. In this study, the magnetic Fe 3 O 4 nanospheres coupled with mouse antihuman IgG (Ab1 IgG ) were used as an immune capture probe (Fe 3 O 4 @Ab1 IgG ) to capture and separate the target, and rabbit antihuman IgG (Ab2 IgG ) coupled with highly luminescent quantum dot nanobeads (QBs) as a fluorescence detection probe (QBs@Ab2 IgG ) was used to realize high sensitivity detection. After the formation of a sandwich immunocomplex, the fluorescence intensity of the precipitate after magnetic separation was measured at the excitation wavelength of 370 nm. Under optimal conditions, a wide linear range varying from 0.005 to 40 ng·mL –1 was obtained for the detection of human IgG with a lower limit of detection at 4 pg·mL –1 (S/N = 3). The recoveries of intra- and interassays were 90.0–101.9 and 96.0–106.6%, respectively, and the relative standard deviations were 6.3–10.2 and 2.6–10.5%, respectively. Furthermore, the proposed method was successfully demonstrated to detect human IgG in serum samples, and the detection results were not statistically different ( P > 0.05) from commercial enzyme-linked immunosorbent assay kits. This method is sensitive, fast, and accurate, which could be expanded to detect the specific IgM and IgG antibodies against SARS-CoV-2.

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Section: Resultsmentioning

confidence: 99%

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres

et al. 2020

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“…Shrimp (Litopenaeus vannamei) was obtained from a local fish market, Qingdao, Shandong Province, China. The sequence of the tropomyosin-binding aptamer (Zhang et al 2018) 5′-TAC TAA CGG TAC AAG CTA CCA GGC CGC CAA CGT TGA CCT AGA AGC ACT GCC AGA CCC GAA CGT TGA CCT AGA AGC-3′, was chemically synthesized by Sangon Biotechnology Co., Ltd. (Shanghai, China), further dissolved in ultrapure water, then stored at 4 °C prior to use. The tropomyosin-binding aptamer was denoted as TMBA.…”

Section: Methodsmentioning

confidence: 99%

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

Pavase

Lin

Soomro

et al. 2021

Mar Life Sci Technol

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A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin (TM), an important biomarker responsible for severe clinical reactivity to shellfish. In a gold nanoparticles (AuNPs)-tropomyosin-binding aptamer (TMBA) complex, the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM. In addition, AuNPs tend to aggregate in the presence of ionic salt, revealing a color change (i.e., wine-red to purple/ blue) with a shift in the maximum absorption peak from 520 nm. In the presence of specific binding TM, the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone. Based on the aggregation and sensitive spectral transformation principle, the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples. The reliability, selectivity, and sensitivity of the aptasensor was then tested with food samples spiked with TM. The observed detection limit was as low as 70 nmol/L in shrimp, 90 nmol/L in tofu, and 80 nmol/L in eggs, respectively. We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.

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“…The development of aptamer-based gold nanorod and magnetic nanoparticle fluorescence assay has been reported to detect ochratoxin A in grape juice samples (a food mycotoxin causing allergy) and allergens in different food matrices [206,207]. Moreover, other nanomaterials such as polyanhydride nanoparticles, quantum dots, and dendrimers proved to be efficient oral vehicles for immunotherapy against experimental peanut allergies [208].…”

Section: Nanomaterials For Protection From Allergensmentioning

confidence: 99%

An Overview of the Applications of Nanomaterials and Nanodevices in the Food Industry

Shafiq

Anjum

Hano

et al. 2020

Foods

172

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The efficient progress in nanotechnology has transformed many aspects of food science and the food industry with enhanced investment and market share. Recent advances in nanomaterials and nanodevices such as nanosensors, nano-emulsions, nanopesticides or nanocapsules are intended to bring about innovative applications in the food industry. In this review, the current applications of nanotechnology for packaging, processing, and the enhancement of the nutritional value and shelf life of foods are targeted. In addition, the functionality and applicability of food-related nanotechnologies are also highlighted and critically discussed in order to provide an insight into the development and evaluation of the safety of nanotechnology in the food industry.

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Magnetic-assisted aptamer-based fluorescent assay for allergen detection in food matrix

Cited by 54 publications

References 35 publications

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

An Overview of the Applications of Nanomaterials and Nanodevices in the Food Industry

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Magnetic-assisted aptamer-based fluorescent assay for allergen detection in food matrix

Cited by 54 publications

References 35 publications

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe3O4 Nanospheres

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe3O4 Nanospheres

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

An Overview of the Applications of Nanomaterials and Nanodevices in the Food Industry

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Product

Resources

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Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres

Highly Sensitive Fluorescence-Linked Immunosorbent Assay for the Determination of Human IgG in Serum Using Quantum Dot Nanobeads and Magnetic Fe₃O₄ Nanospheres