Magnetic AuNP@Fe 3 O 4 nanoparticles as reusable carriers for reversible enzyme immobilization

Cao, Yonghao; Wen, Liyin; Švec, František; Tan, Tianwei; Lv, Yongqin

doi:10.1016/j.cej.2015.10.075

Cited by 104 publications

(30 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A aplicação do ensaio foi realizada com a mioglobina para ambos os biorreatores, enquanto as digestões da albumina sérica bovina (BSA) e do citocromo c foram feitas somente no biorreator revestido com ouro. 108 De acordo com os autores, a digestão da mioglobina foi alcançada em 15 minutos com 11 peptídeos identificados e cobertura sequencial de 68,8% para o biorreator revestido com Cu 2+ e, para o biorreator revestido com ouro, 15 peptídeos identificados com cobertura sequencial de 93,8%. Para a digestão com citocromo c e BSA foram identificados 10 peptídeos com cobertura sequencial de 90,9% para o citocromo c e 33 peptídeos com cobertura sequencial de 54,1% para a BSA.…”

Section: Suportes Para Imobilizaçãounclassified

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

et al. 2020

View full text Add to dashboard Cite

Recebido em 25/11/2019; aceito em 28/01/2020; publicado na web em 16/04/2020 ENZYME IMMOBILIZATION: CONCEPTS AND EFFECTS ON PROTEOLYSIS. In recent years, great advances have been observed in the proteomic area regarding the different approaches adopted as top-down, middle-down and bottom-up. Regarding middle-down and bottom-up approaches, a previous step for protein digestion is necessary for further analysis of the generated peptides. Classical digestion protocols employing proteases are usually carried out in solution, which can lead to a number of disadvantages such as high digestion time, enzymatic autolysis and impossibility to reuse the enzymes. In view of these drawbacks, different approaches have been studied and applied for improving enzymatic efficiency, inhibition of autolysis, reduction of digestion time, and to minimize the number of missed cleavages. In the literature, great efforts have been made in order to achieve maximum efficiency in enzymatic digestion, with emphasis on proteases immobilization. In this review, concepts related to protein digestion, immobilization techniques, as well as the main support used for enzyme immobilization are discussed considering new tools for proteomic analysis.

show abstract

Section: Suportes Para Imobilizaçãounclassified

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The GNP‐mediate enzyme reactor showed higher activity compared to capillaries in which the enzyme was directly bonded. Similarly, GNPs were used as convenient reactive surface for immobilization of trypsin on magnetic beads . GNPs were in‐situ prepared by reduction of HAuCl4 with NaBH4 on citric acid‐coated magnetic Fe3O4 nanoparticles.…”

Section: Gnps In Sample Preparationmentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

View full text Add to dashboard Cite

Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

show abstract

“…During the past decades, Au nanocatalysts have been intensively investigated, due to the vital role in industrially relevant reactions, such as preferential hydrocarbon combustion, oxidation of CO, propylene epoxidation, methanol synthesis, environmental catalysis and hydrogenation reactions . As is well known, Au NPs loaded on metal oxide supports with high surface area have demonstrated their high catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of double‐shelled CeO₂‐La₂O₃/Au/Fe₃O₄ hollow architecture as a recyclable and highly thermal stability nanocatalyst

Huang

Fang

Zhang

et al. 2017

Applied Organom Chemis

View full text Add to dashboard Cite

A novel magnetic binary‐metal‐oxide‐coated nanocataly composing of a hollow Fe3O4 core and CeO2‐La2O3 shells with Au nanoparticles encapsulated has been created in this work. The structural features of catalysts were characterized by several techniques, including SEM, TEM, UV‐vis, FTIR, XRD, XPS and TGA analyses. After the coating of CeO2‐La2O3 layer, CeO2‐La2O3/Au/C/Fe3O4 microspheres showed a superior thermal stability and catalytic reactivity compared with a pure CeO2 or La2O3 layer. Accompanied by the burning of carbon layer, the specific surface could be increased by the formation of double‐shelled structure. Besides, the desired samples could be separated by magnet, implying the superior recycle performance. Using the reduction of 4‐nitrophenol by NaBH4 as a model reaction, the microspheres exhibited highly reusability, superior catalytic activity, thermal stability, which are attributed to the unique double‐shelled structure of the support, uniform distribution of Au nanoparticles, the highly thermal stability of CeO2‐La2O3 layer and mixed oxide synergistic effect. As a consequence, the unique nanocatalyst will open a promising way in the fabrication of the double‐shelled hollow binary‐metal‐oxide materials for future research and has great potential in other applications.

show abstract

Magnetic AuNP@Fe 3 O 4 nanoparticles as reusable carriers for reversible enzyme immobilization

Cited by 104 publications

References 44 publications

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

Functionalized gold nanoparticles for sample preparation: A review

Fabrication and characterization of double‐shelled CeO₂‐La₂O₃/Au/Fe₃O₄ hollow architecture as a recyclable and highly thermal stability nanocatalyst

Contact Info

Product

Resources

About

Magnetic AuNP@Fe 3 O 4 nanoparticles as reusable carriers for reversible enzyme immobilization

Cited by 104 publications

References 44 publications

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

Imobilização Enzimática: Conceito E Efeitos Na Proteólise

Functionalized gold nanoparticles for sample preparation: A review

Fabrication and characterization of double‐shelled CeO2‐La2O3/Au/Fe3O4 hollow architecture as a recyclable and highly thermal stability nanocatalyst

Contact Info

Product

Resources

About

Fabrication and characterization of double‐shelled CeO₂‐La₂O₃/Au/Fe₃O₄ hollow architecture as a recyclable and highly thermal stability nanocatalyst