Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment

Abstract

BackgroundPatients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems. However, little is known of the effect of periodontitis on orthodontic treatment. Thus, we compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides.ResultsSaliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. Nine mass peaks showed significant differences. Orthodontic patients in the group without periodontal disease showed higher mass peaks for seven peptides of the nine, whereas the mass peaks for the other two peptides were higher in the periodontal-orthodontic patients. Besides, these differentially-expressed peptides were sequenced.ConclusionsThe elucidated candidate biomarkers indicated interactions between periodontal condition and orthodontic treatment and their contributions to the changes of saliva protein profiles. Our results provide novel insight into the altered salivary protein profile during periodontal-orthodontic treatment, and may lead to the development of a therapeutic monitoring strategy for periodontics and orthodontics.

Multi-omics profiling reveals that eating difficulties developed consecutively to artificial nutrition in the neonatal period are associated to specific saliva composition

“…1 H NMR proved useful to identify different metabolite signatures in children with and without caries [24]. As to MALDI-TOF profiling, it was combined to magnetic bead sample treatment and allowed to identify markers of periodontal disease in subjects with or without orthodontic treatment [25]. In all these studies, the patients were carefully selected based on homogenous clinical criteria, and gross medical confounding conditions were excluded.…”

Salivary Peptidomics Targeting Clinical Applications

“…Similar to SELDI-TOF MS profiling, magnetic RP beads and Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS have been used for salivary peptidome characterization under pathophysiological conditions, as, for example, in the evaluation of periodontal-orthodontic treatment (32). However, the information retrieved using these strategies is limited requiring complementary bottom-up approaches.…”

“…For instance, Zhang et al (32,44) used magnetic beads combined with MALDI-MS for saliva profiling in response to orthodontic procedures, solid foods, and teeth eruption, but only few MS peaks were significantly distinct among the conditions (27). While some quantitative methods developed for proteome studies were inherited, peptidome analysis has its own quantification traits.…”

Rapid diagnosis of periodontitis, a feasibility study using MALDI-TOF mass spectrometry

“…In another study, the same authors found that peptide profiles of GCF from periodontal maintenance subjects could be used to predict sites with attachment loss [27]. Zhang et al compared the protein profiles of orthodontics patients with and without periodontitis using MALDI-TOF MS and found significantly different intensities of nine peaks, seven of which were higher in healthy subjects [28]. Chaiyarit et al analyzed salivary protein profiles in oral cancer, oral lichen planus, and chronic periodontitis and found that mass signals at 5,835.73 and 9,801.83 Da were significantly decreased in periodontitis patients compared to the two others oral diseases [29].…”