Salivary Peptidomics Targeting Clinical Applications

Vitorino, Rui; Ferreira, Rita; Caseiro, Armando; Amado, Francisco

doi:10.1016/b978-0-444-62650-9.00009-9

Cited by 3 publications

(6 citation statements)

References 87 publications

(66 reference statements)

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“…Compared with other body fluids with clinical relevance ( e.g. , blood, urine, cerebrospinal fluid), low-molecular-weight peptides account for a higher fraction (∼30%) of total proteinaceous content of saliva ( 37 ), what makes the recovery of small peptides easier and faster than conventional bottom-up proteomic analyses.…”

Section: Discussionmentioning

confidence: 99%

“…A variety of approaches, including the use of organic solvents ( e.g. , methanol, MeCN), acids (TFA, HCl), chaotropic agent (urea), ultrafiltration spin devices, and chromatography, have been shown to be feasible in the extraction of endogenous peptides from complex matrixes ( 37 ). Different methods can bias the extraction toward intrinsic physicochemical features of peptides likely affecting the yield and purity or even leading to the enrichment of structurally modified bioactive peptides ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

“…Compared to other body fluids with clinical relevance (e.g. blood, urine, cerebrospinal fluid) low-molecular weight peptides account to a higher fraction (~30%) of total proteinaceous content of saliva(37), what makes the recovery of small peptides easier and faster than conventional bottom-up proteomic analyses.A variety of approaches, including the use of organic solvents (e.g. methanol, acetonitrile), acids (TFA, HCl), chaotropic agent (urea), ultrafiltration spin devices and chromatography, has shown feasible in the extraction of endogenous peptides from complex matrixes(37).…”

mentioning

confidence: 99%

“…blood, urine, cerebrospinal fluid) low-molecular weight peptides account to a higher fraction (~30%) of total proteinaceous content of saliva(37), what makes the recovery of small peptides easier and faster than conventional bottom-up proteomic analyses.A variety of approaches, including the use of organic solvents (e.g. methanol, acetonitrile), acids (TFA, HCl), chaotropic agent (urea), ultrafiltration spin devices and chromatography, has shown feasible in the extraction of endogenous peptides from complex matrixes(37). Different methods can bias the extraction towards intrinsic physicochemical features of peptides likely affecting the yield, purity or even the leading to the enrichment of structurally modified bioactive peptides(38).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Peptidomics-Driven Strategy Reveals Peptides and Predicted Proteases Associated With Oral Cancer Prognosis

Neves

Granato

Busso-Lopes

et al. 2021

Molecular & Cellular Proteomics

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Protease activity has been associated with pathological processes that can lead to cancer development and progression. However, understanding the pathological unbalance in proteolysis is challenging since changes can occur simultaneously at protease, their inhibitor and substrate levels. Here, we present a pipeline that combines peptidomics, proteomics and peptidase predictions for studying proteolytic events in the saliva of seventy-nine patients and their association with oral squamous cell carcinoma (OSCC) prognosis. Our findings revealed differences in the saliva peptidome of patients with (pN+) or without (pN0) lymph node metastasis and delivered a panel of ten endogenous peptides correlated with poor prognostic factors plus five molecules able to classify pN0 and pN+ patients (ROC-AUC>0.85). In addition, endo- and exopeptidases putatively implicated in the processing of differential peptides were investigated using cancer tissue gene expression data from publicly repositories reinforcing their association with poorer survival rates and prognosis in oral cancer. The dynamics of the OSCC-related proteolysis was further explored via the proteomic profiling of saliva. This revealed that peptidase/endopeptidase inhibitors exhibited reduced levels in the saliva of pN+ patients, as confirmed by SRM-MS, whilst minor changes were detected in the level of saliva proteases. Taken together, our results indicated that proteolytic activity is accentuated in the saliva of OSCC patients with lymph node metastasis and, at least in part, this is modulated by reduced levels of salivary peptidase inhibitors. Therefore, this integrated pipeline provided better comprehension and discovery of molecular features with implications in the oral cancer metastasis prognosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Peptidomics-Driven Strategy Reveals Peptides and Predicted Proteases Associated With Oral Cancer Prognosis

Neves

Granato

Busso-Lopes

et al. 2021

Molecular & Cellular Proteomics

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“…However, the aforementioned methods require training and are time‐consuming in large‐scale screening, and insufficient for complex immune‐inflammatory diseases, such as periodontitis. As the amount and profile of peptides are modulated by physiological and pathological states, proteomics and peptidomics can be used to detect specific markers to obtain a comprehensive understanding of cellular pathways modulated by diseases (Menschaert et al., 2010; Michael Schrader et al., 2014; Vitorino et al., 2014). MALDI‐TOF MS based on magnetic beads has the characteristics of easy sample preparation, low sample consumption, high sensitivity, and a high degree of automation in high‐throughput analyses of a complex biological sample, which means great clinical application value (Cho et al., 2015; Hajduk et al., 2016).…”

Section: Discussionmentioning

confidence: 99%

Peptidomic changes of saliva after non‐surgical treatment of stage I/II generalized periodontitis

Yuan

Tong

et al. 2021

Oral Diseases

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Objective To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non‐surgical periodontal treatment in patients with generalized periodontitis (stage I/II). Subjects and methods Saliva, serum, and GCF samples were collected from 17 patients at baseline (T0), one week after ultrasonic supragingival scaling (T1) and eight weeks after subgingival scaling and root planning (T2). Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) was carried out to detect changes in peptidomic profiles. Then, nano‐liquid chromatography‐electrospray ionization‐tandem mass spectrometry (nano‐LC/ESI‐MS/MS) was performed to identify potential peptide biomarkers. Results Most of the peptides from the patients exhibited a decreasing trend from the time point of pretreatment to that of post‐treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I/II generalized periodontitis. Seven of these peptides were successfully identified as α‐1‐antitrypsin, immunoglobulin κ variable 4‐1, haptoglobin, and immunoglobulin heavy constant γ2. Conclusions Certain peptides in saliva, serum, and GCF were down‐regulated after non‐surgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.

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