Magnetic Carbon Nanotubes for Protein Separation

Diao, Xiuhui; Chen, Hongyu; Zhang, Guoliang; Zhang, Fengbao; Fan, Xiaobin

doi:10.1155/2012/806019

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…Carbon nanotubes and other nanostructured porous materials have received a lot of attention in recent years due to their remarkable optical, electronic, thermal and mechanical properties. As a consequence, these materials have been suggested for use in an enormous range of applications including the desalination of sea water; the removal of dangerous contaminants from water supplies; the separation of gases, ions and biomolecules; the sequencing of DNA; electrical devices and biosensors; and nanofluidic devices . Many of these applications involve flowing liquids or gases through the interior of the pores.…”

Section: Introductionmentioning

confidence: 99%

What Have We Learnt About the Mechanisms of Rapid Water Transport, Ion Rejection and Selectivity in Nanopores from Molecular Simulation?

Thomas

Corry

Hilder

2014

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157

109

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Nanopores have demonstrated an extraordinary ability to allow water molecules to pass through their interiors at rates far exceeding expectations based on continuum theory. Moreover, simulation studies suggest that particular nanoscale pores have the potential to discriminate between water and salts as well as to distinguish between a range of different ion types. Some of the unusual features of transport in these nanopores have been elucidated with molecular dynamics simulation, specifically the spontaneous filling and rapid transport of water, the rejection of ions and the selection between ions. The main focus of this review, however, is the physical mechanisms which act to produce such remarkable behaviour at this scale, drawing on the many studies that have been conducted in the last decade. Since molecular dynamics simulations allow the motion of individual atoms to be followed over time, they have the potential to provide fundamental insight into the reasons why transport in nanoscale pores differs from expectations based on macroscopic theory. Gaining an understanding of the mechanisms of transport in these tiny pores should guide future experiments in this area aimed at developing novel technologies and improving existing membrane separation techniques.

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Section: Introductionmentioning

confidence: 99%

What Have We Learnt About the Mechanisms of Rapid Water Transport, Ion Rejection and Selectivity in Nanopores from Molecular Simulation?

Thomas

Corry

Hilder

2014

Small

157

109

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“…In addition, Zhang et al successfully transported telomerase inhibited small interference RNA into tumor cells and suppressed their growth[ 52 ]. However, researchers generally used protein fictionalization for stabilizing [ 53 ], labeling[ 54 ] and separating[ 55 ] CNTs rather than transporting functional protein to tune cellular behavior. Here, we were able to conjugate protein to the CNT-PLGA complex.…”

Section: Resultsmentioning

confidence: 99%

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

et al. 2013

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Cancer has arisen to be of the most prominent health care issues across the world in recent years. Doctors have used physiological intervention as well as chemical and radioactive therapeutics to treat cancer thus far. As an alternative to current methods, gene delivery systems with high efficiency, specificity, and safety that can reduce side effects such as necrosis of tissue are under development. Although viral vectors are highly efficient, concerns have arisen from the fact that viral vectors are sourced from lethal diseases. With this in mind, rod shaped nano-materials such as carbon nanotubes (CNTs) have become an attractive option for drug delivery due to the enhanced permeability and retention effect in tumors as well as the ability to penetrate the cell membrane. Here, we successfully engineered poly (lactic-co-glycolic) (PLGA) functionalized CNTs to reduce toxicity concerns, provide attachment sites for pro-apoptotic protein caspase-3 (CP3), and tune the temporal release profile of CP3 within bone cancer cells. Our results showed that CP3 was able to attach to functionalized CNTs, forming CNT-PLGA-CP3 conjugates. We show this conjugate can efficiently transduce cells at dosages as low as 0.05 μg/ml and suppress cell proliferation up to a week with no further treatments. These results are essential to showing the capabilities of PLGA functionalized CNTs as a non-viral vector gene delivery technique to tune cell fate.

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“…After magnetic decantation, a separation capacity of 56 μ g/mg for the CSCNTs-APBA-IgG conjugate was achieved. The conjugate was also successful in capturing target antigens through antigen antibody reactions [60].…”

Section: Carbon Nanotubes (Cnts)mentioning

confidence: 99%

Nanoenabled Bioseparations: Current Developments and Future Prospects

Fahmy

Alawak

Brüßler

et al. 2019

BioMed Research International

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The use of nanomaterials in bioseparations has been recently introduced to overcome the drawbacks of the conventional methods. Different forms of nanomaterials, particularly magnetic nanoparticles (MNPs), carbon nanotubes (CNTs), casted nanoporous membranes, and electrospun nanofiber membranes were utilized in biological separation for the aim of production of different biomolecules such as proteins, amino acids, nucleic acids, and enzymes. This paper critically reviews the state-of-the-art efforts undertaken in this regard, with emphasis on the synthesis and performance evaluation of each nanoform. Challenges and future prospects in developing nanoenabled bioseparations are also discussed, for the purpose of highlighting potential advances in the synthesis and fabrication of novel nanomaterials as well as in the design of efficient nanoenabled processes for separating a wide spectrum of biomolecules.

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Magnetic Carbon Nanotubes for Protein Separation

Cited by 13 publications

References 32 publications

What Have We Learnt About the Mechanisms of Rapid Water Transport, Ion Rejection and Selectivity in Nanopores from Molecular Simulation?

What Have We Learnt About the Mechanisms of Rapid Water Transport, Ion Rejection and Selectivity in Nanopores from Molecular Simulation?

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

Nanoenabled Bioseparations: Current Developments and Future Prospects

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