Magnetic field assisted microcontact printing: A new concept of fully automated and calibrated process

Cau, Jean-Christophe; Ludovic, Lafforgue; Nogues, Marie; Lagraulet, Adriana; Paveau, Vincent

doi:10.1016/j.mee.2013.03.164

Cited by 12 publications

(8 citation statements)

References 23 publications

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“…Once inked on the micropatterned magnetic MacroStamp TM , the oligonucleotides were transferred on a solid surface of interest after applying a precise and homogeneous force. The magnetic field was generated from the bottom of the printing area by a set of magnets ensuring an accurate transfer of inked patterns [31]. …”

Section: Resultsmentioning

confidence: 99%

Automated and Multiplexed Soft Lithography for the Production of Low-Density DNA Microarrays

Fredonnet

Foncy

Cau³

et al. 2016

Microarrays

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Microarrays are established research tools for genotyping, expression profiling, or molecular diagnostics in which DNA molecules are precisely addressed to the surface of a solid support. This study assesses the fabrication of low-density oligonucleotide arrays using an automated microcontact printing device, the InnoStamp 40®. This automate allows a multiplexed deposition of oligoprobes on a functionalized surface by the use of a MacroStampTM bearing 64 individual pillars each mounted with 50 circular micropatterns (spots) of 160 µm diameter at 320 µm pitch. Reliability and reuse of the MacroStampTM were shown to be fast and robust by a simple washing step in 96% ethanol. The low-density microarrays printed on either epoxysilane or dendrimer-functionalized slides (DendriSlides) showed excellent hybridization response with complementary sequences at unusual low probe and target concentrations, since the actual probe density immobilized by this technology was at least 10-fold lower than with the conventional mechanical spotting. In addition, we found a comparable hybridization response in terms of fluorescence intensity between spotted and printed oligoarrays with a 1 nM complementary target by using a 50-fold lower probe concentration to produce the oligoarrays by the microcontact printing method. Taken together, our results lend support to the potential development of this multiplexed microcontact printing technology employing soft lithography as an alternative, cost-competitive tool for fabrication of low-density DNA microarrays.

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Section: Resultsmentioning

confidence: 99%

Automated and Multiplexed Soft Lithography for the Production of Low-Density DNA Microarrays

Fredonnet

Foncy

Cau³

et al. 2016

Microarrays

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“…After UV exposure of the resist layer (SU-8 3050, thickness of 25 μm), the master mold was developed in SU-8 developer for 9 minutes and rinsed using isopropanol. Then a PerFluorodecylTrichloroSilane (PFTS) coating was performed by spray pyrolysis deposition (SPD) to confer anti-adhesive properties to the mold surface [ 19 ]. Similar to the fabrication of the microfluidic devices, structured magnetic PDMS stamps were obtained in two steps.…”

Section: Methodsmentioning

confidence: 99%

Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays

Foncy¹,

Estève²,

Degache³

et al. 2018

PLoS ONE

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Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extracellular matrix proteins for the fabrication of cell microarrays. We have developed a microfluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm2 through the control of both the flow rate and the wetting mechanism of the stamp surface during microfluidic inking. Thanks to the reproducibility and integration capabilities provided by microfluidics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays.

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“…Automated microcontact printing for microarray applications was lately introduced by Gesim, Germany and by Biosoft Technolgies (Toulouse, France; biosoftlab.com). The InnoStamp 40 from Biosoft Technologies uses magnetic stamps which are inked, dried, aligned and finally printed with the help of a strong magnet under the printing area [ 76 ].…”

Section: Up-to-date Patterning Of Bresmentioning

confidence: 99%

Analytical Protein Microarrays: Advancements Towards Clinical Applications

Sauer

2017

Sensors

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Protein microarrays represent a powerful technology with the potential to serve as tools for the detection of a broad range of analytes in numerous applications such as diagnostics, drug development, food safety, and environmental monitoring. Key features of analytical protein microarrays include high throughput and relatively low costs due to minimal reagent consumption, multiplexing, fast kinetics and hence measurements, and the possibility of functional integration. So far, especially fundamental studies in molecular and cell biology have been conducted using protein microarrays, while the potential for clinical, notably point-of-care applications is not yet fully utilized. The question arises what features have to be implemented and what improvements have to be made in order to fully exploit the technology. In the past we have identified various obstacles that have to be overcome in order to promote protein microarray technology in the diagnostic field. Issues that need significant improvement to make the technology more attractive for the diagnostic market are for instance: too low sensitivity and deficiency in reproducibility, inadequate analysis time, lack of high-quality antibodies and validated reagents, lack of automation and portable instruments, and cost of instruments necessary for chip production and read-out. The scope of the paper at hand is to review approaches to solve these problems.

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Magnetic field assisted microcontact printing: A new concept of fully automated and calibrated process

Cited by 12 publications

References 23 publications

Automated and Multiplexed Soft Lithography for the Production of Low-Density DNA Microarrays

Automated and Multiplexed Soft Lithography for the Production of Low-Density DNA Microarrays

Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays

Analytical Protein Microarrays: Advancements Towards Clinical Applications

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