Magnetic field exposure enhances mRNA expression of σ32 in E. coli

Cairo, Paulo Araquém Ramos; Greenebaum, Ben; Goodman, E. M.

doi:10.1002/(sici)1097-4644(19980101)68:1<1::aid-jcb1>3.3.co;2-9

Cited by 11 publications

(13 citation statements)

References 11 publications

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“…The unexpected effect of magnetic field exposure on DNA stability prompted us to further our investigation in order to find out the mechanism involved. In this regard, research of Cairo et al [10] showing that when E. coli cultures are exposed to a magnetic field (60 Hz) of 1.2 mT, the expression of δ 32 , a transcription factor that guides RNA polymerase to interact with a variety of stress gene promoters, would be enhanced directed us to consider the potential role of heat shock proteins. As a matter of fact, there were three lines of research results that had inspired us.…”

Section: Resultsmentioning

confidence: 99%

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Chow

Tung

2000

FEBS Letters

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In contrast to the common impression that exposure to a magnetic field of low frequency causes mutations to organisms, we have demonstrated that a magnetic field can actually enhance the efficiency of DNA repair. Using Escherichia coli strain XL-1 Blue as the host and plasmid pUC8 that had been mutagenized by hydroxylamine as the vector for assessment, we found that bacterial transformants that had been exposed to a magnetic field of 50 Hz gave lower percentages of white colonies as compared to transformants that had not been exposed to the magnetic field. This result was indicative that the efficiency of DNA repair had been improved. The improvement was found to be mediated by the induced overproduction of heat shock proteins DnaK/J (Hsp70/40). ß

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Section: Resultsmentioning

confidence: 99%

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Chow

Tung

2000

FEBS Letters

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“…LI‐EMF induced a total of 188, 63, and 60 transcription factors in the treated samples with recovery times of 0 h, 3 h, and 6 h. As the time increased after LI‐EMF treatment, the number of elicited transcription factors declined significantly, which indicated that the transcription factor repaired the influence of LI‐EMF on the cells of I. lacteus . Cairo et al [] found that ELF‐MF (1.1 mT, 60 Hz) could stimulate the δ 32 expression, a stress promoter transcription factor in E. coli . In addition, several glutathione S‐transferases (GSTs), generated under diverse stress conditions [Collinson and Grant, ], were elicited by LI‐EMF in the present study.…”

Section: Discussionmentioning

confidence: 99%

Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐intensity electromagnetic field

Sun

et al. 2019

Bioelectromagnetics

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A polysaccharide of Irpex lacteus, a white‐rot fungus with lignocellulose‐degrading activities, has been used as a commercial medicine for nephritis treatment. Previously, a low‐intensity electromagnetic field (LI‐EMF) was found to increase the biomass and polysaccharide content of Irpex lacteus and induce twists on the cell surface. In this study, RNA‐sequencing (RNA‐seq) technology was used to analyze the underlying mechanism of LI‐EMF's influence on Irpex lacteus. We identified 3268, 1377, and 941 differentially expressed genes (DEGs) in the LI‐EMF‐treated samples at recovery times of 0 h, 3 h, and 6 h, respectively, indicating a significant decline in the influence of the LI‐EMF treatment on Irpex lacteus with the passage of recovery time. Moreover, 30 upregulated and 14 downregulated DEGs overlapped in the LI‐EMF‐treated samples at the recovery times of 0 h, 3 h, and 6 h, implying the important lasting effects of LI‐EMF. The reliability of the RNA‐seq data were validated by quantitative real‐time PCR (qRT‐PCR). The DEGs related to transcription factors, cell proliferation, cell wall, membrane components, amino acid biosynthesis and metabolism, and polysaccharide biosynthesis and metabolism were significantly enriched in the LI‐EMF‐treated samples. The experiments confirmed that the LI‐EMF treatment significantly increased the content of amino acids with a considerable increase in the content of essential amino acids. Therefore, the global gene expression changes explained the pleiotropic effects of Irpex lacteus induced by the LI‐EMF treatment. These findings provide the requisite data for the appropriate design and application of LI‐EMF in the fermentation of microorganisms to increase production. Bioelectromagnetics. 40:104–117, 2019. © 2019 Bioelectromagnetics Society

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“…Two pieces of information are consistent with this hypothesis. First, Cairo et al, [1998] have shown that the intracellular level of mRNA speci®c for s 32 , a protein that interacts with RNA polymerase to help it recognise a variety of stress promoters in the cells, is enhanced under electric ®eld conditions. Second, our results of electrophoretical analysis of total cell proteins have shown quantitative and qualitative differences between protein pro®les obtained out of sham and bacteria growing under electric ®eld conditions [Grzesiuk et al, 2000;Laubitz et al, 2000].…”

Section: Discussionmentioning

confidence: 99%

Influence of intestinal myoelectrical activity on the growth of Escherichia coli

Grzesiuk

Laubitz²,

Sikora

et al. 2001

Bioelectromagnetics

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Intestinal bacteria, particularly those adhering to intestinal epithelial cells, are exposed to electric fields and currents generated by the muscular activity of the small intestine. This activity displays a regular pattern known as the myoelectrical migrating complex (MMC). In order to explore the possibility that these endogenous electric fields could affect bacterial growth, a digitised duodenal signal obtained via serosal electrodes from a healthy calf was recorded and then applied via platinum electrodes to Escherichia coli cultures. The culture tubes were placed within a Faraday shield, incubated at 37 degrees C with shaking, and stimulated by the electric current for 5 or 8 h. The growth of E. coli stimulated by the electric current was significantly altered compared to those of non-stimulated controls: after a period of intensive growth, inhibition of cell division was observed. This was not the case when the bacteria with lon mutation were used. Moreover, synchronic bacterial culture could not be achieved in the presence of the MMC-related electric field. These results suggest that the myoelectrical activity of the duodenum, through action on cell membrane, can affect cell division of intestinal bacteria.

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Magnetic field exposure enhances mRNA expression of σ32 in E. coli

Cited by 11 publications

References 11 publications

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐intensity electromagnetic field

Influence of intestinal myoelectrical activity on the growth of Escherichia coli

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Magnetic field exposure enhances mRNA expression of σ32 in E. coli

Cited by 11 publications

References 11 publications

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Magnetic field exposure enhances DNA repair through the induction of DnaK/J synthesis

Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐­intensity electromagnetic field

Influence of intestinal myoelectrical activity on the growth of Escherichia coli

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Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐intensity electromagnetic field