“…Cleavage under targets and tagmentation (CUT&Tag) technology is a new DNA-protein interaction research method, which is expected to replace the traditional ChIP-seq technology to explore histone modification regions, transcription factor binding status, and DNA-protein interaction in the whole genome [ 174 ]. CUT&Tag is favored by epigenetic researchers because of its advantages such as simple operation (without immune co-precipitation and ultrasonic fragmentation), low initial cell volume (as low as 60 cells), high signal-to-noise ratio, high sensitivity, good repeatability and low cost [ 175 ]. The core of CUT&Tag technology is the transposon pAG-Tn5, which is fused with the protein A/G antibody binding domain on Tn5 [ 176 ].…”