Magnetic resonance imaging of autologous chondrocyte implantation

Alparslan, Leyla; Minas, Tom; Winalski, Carl S.

doi:10.1016/s0887-2171(01)90025-3

Cited by 83 publications

(94 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The use of the HFBR model system is particularly relevant to the study of both normal and diseased articular cartilage since the tissue that forms is typical hyaline cartilage with abundant type II collagen messenger RNA expression (4). In addition, the neocartilage formation is focal in nature (i.e., it occurs in a small defined region within the bioreactor), which models the situation that might occur either in early OA lesions or in reparative cartilage resulting from transplantation (15,16).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the ability of MRI to assess the biochemical properties of cartilage undergoing focal changes is still under investigation. Previous work has indicated that MR images of autologous transplants may show variations that have clinical significance, but no biochemical evaluations have been performed (15,16). Finally, the unique environment of the HFBR for supporting cartilage formation needs further characterization.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Matrix fixed‐charge density as determined by magnetic resonance microscopy of bioreactor‐derived hyaline cartilage correlates with biochemical and biomechanical properties

Chen¹,

Fishbein²,

Torzilli³

et al. 2003

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To use noninvasive magnetic resonance imaging (MRI), biochemical analyses, and mechanical testing of engineered neocartilage grown in a hollowfiber bioreactor (HFBR) to establish tissue properties, and to test the hypothesis that MRI can be used to monitor biochemical and biomechanical properties of neocartilage.Methods. Chondrocytes from day 16 embryonic chick sterna were inoculated into an HFBR and maintained for up to 4 weeks with and without exposure to chondroitinase ABC. The fixed-charge density (FCD) of the cartilage was determined using the MRI gadolinium exclusion method. The sulfated glycosaminoglycan (S-GAG), hydroxyproline, and DNA contents were determined using biochemical procedures, while dynamic and equilibrium moduli were determined from mechanical indentation tests.Results. S-GAG content, tissue cross-sectional area, and equilibrium modulus of the neocartilage increased with development time. There was a gradient of S-GAG content across the length of control neocartilage at the 4-week time point, with higher values being found toward the inflow region. Exposure to chondroitinase ABC resulted in a decrease in tissue area, negative FCD, proteoglycan content, and equilibrium and dynamic moduli. The treated bioreactors displayed a lengthwise variation in S-GAG content, with higher values toward the outflow end. Linear correlations were established among FCD, proteoglycan content, and biomechanical properties.Conclusion. HFBR-derived neocartilage showed regional variation in S-GAG content under control conditions, and in the decrease of S-GAG in response to enzyme treatment. In addition, the results support the hypothesis that tissue parameters derived from MRI can be used to noninvasively monitor focal neocartilage formation and biochemical and biomechanical properties.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Matrix fixed‐charge density as determined by magnetic resonance microscopy of bioreactor‐derived hyaline cartilage correlates with biochemical and biomechanical properties

Chen¹,

Fishbein²,

Torzilli³

et al. 2003

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…In the uncomplicated case, there is a gradual change in signal characteristics of reparative tissue over time to resemble those of normal articular cartilage, and filling of the chondral defect should be identified [41] (Fig. 7).…”

Section: Mri Of Autologous Chondrocyte Implantationmentioning

confidence: 99%

“…8). The significance of subchondral oedema has not been determined, but some groups have suggested it predicts poor outcome if identified at over 1-year post-operatively [38,41]. The presence of bone marrow oedema beneath the graft does not correlate with the histological nature of the reparative tissue [37].…”

Section: Mri Of Autologous Chondrocyte Implantationmentioning

confidence: 99%

MR imaging of autologous chondrocyte implantation of the knee

et al. 2006

View full text Add to dashboard Cite

Autologous chondrocyte implantation (ACI) is a surgical technique that is increasingly being used in the treatment of full-thickness defects of articular cartilage in the knee. It involves the arthroscopic harvesting and in vitro culture of chondrocytes that are subsequently implanted into a previously identified chondral defect. The aim is to produce a repair tissue that closely resembles hyaline articular cartilage that gradually becomes incorporated, restoring joint congruity. Over the long term, it is hoped that this will prevent the progression of full-thickness articular cartilage defects to osteoarthritis. This article reviews the indications and operative procedure performed in ACI. Magnetic resonance imaging (MRI) sequences that provide optimal visualization of articular cartilage in the post-operative period are discussed. Normal appearances of ACI on MRI are presented along with common complications that are encountered with this technique.

show abstract

“…Although several imaging methods exist for cartilage evaluation, MR imaging of cartilage has been shown to be accurate for the detection of chondral abnormalities [2,3,4,5,6,7,8] and in evaluating cartilage-repair procedures [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural MR imaging techniques of the knee articular cartilage: problems for routine clinical application

Breuseghem

2003

Eur Radiol

View full text Add to dashboard Cite

The high incidence of cartilage lesions together with new surgical treatment techniques have necessitated the development of noninvasive cartilage evaluation techniques. Although arthroscopy has been the standard for cartilage evaluation, MR imaging has emerged as the imaging method of choice, allowing morphological evaluation of cartilage and cartilage repair tissue, as well as evaluation of its biochemical content. This article deals with current ultrastructural MR imaging techniques for cartilage evaluation, indicating the advantages as well as the drawbacks for routine clinical application.

show abstract

Magnetic resonance imaging of autologous chondrocyte implantation

Cited by 83 publications

References 19 publications

Matrix fixed‐charge density as determined by magnetic resonance microscopy of bioreactor‐derived hyaline cartilage correlates with biochemical and biomechanical properties

Matrix fixed‐charge density as determined by magnetic resonance microscopy of bioreactor‐derived hyaline cartilage correlates with biochemical and biomechanical properties

MR imaging of autologous chondrocyte implantation of the knee

Ultrastructural MR imaging techniques of the knee articular cartilage: problems for routine clinical application

Contact Info

Product

Resources

About