Magnetic resonance imaging of glioma with novel APTS-coated superparamagnetic iron oxide nanoparticles

Abstract

We report in vitro and in vivo magnetic resonance (MR) imaging of C6 glioma cells with a novel acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (Fe3O4 NPs). In the present study, APTS-coated Fe3O4 NPs were formed via a one-step hydrothermal approach and then chemically modified with acetic anhydride to generate surface charge-neutralized NPs. Prussian blue staining and transmission electron microscopy (TEM) data showed that acetylated APTS-coated Fe3O4 NPs can be taken up by cells. Combined morphological observation, cell viability, and flow cytometric analysis of the cell cycle indicated that the acetylated APTS-coated Fe3O4 NPs did not significantly affect cell morphology, viability, or cell cycle, indicating their good biocompatibility. Finally, the acetylated APTS-coated Fe3O4 nanoparticles were used in magnetic resonance imaging of C6 glioma. Our results showed that the developed acetylated APTS-coated Fe3O4 NPs can be used as an effective labeling agent to detect C6 glioma cells in vitro and in vivo for MR imaging. The results from the present study indicate that the developed acetylated APTS-coated Fe3O4 NPs have a potential application in MR imaging.

Recombinant Interleukin-1 Receptor Antagonist Conjugated to Superparamagnetic Iron Oxide Nanoparticles for Theranostic Targeting of Experimental Glioblastoma

“…injection of the SPION–IL-1Ra resulted in the retention of the particles in the glioma tissue that was observed at T 2 -weighted images and subsequent histologic assay ( Figure 7 ). Previously, it was shown that functionalized particles may penetrate the brain-tumor barrier, enhancing the T 2 -weighted MR scans of the brain tumors [19] , [27] , [45] , [46] . Incorporation of magnetic conjugates was displayed as obvious dark regions in the glioma on T 2 scans in comparison to baseline images (on TurobRARE-T2 and FLASH regimens; Figure 7 A ).…”

Superparamagnetic iron oxide nanoparticles enhance glioma radiosensitivity via inducing cell cycle arrest and apoptosis

“…The amine groups on the surface of APTScoated Fe 3 O 4 NPs were further acetylated via a reaction with acetic anhydride, which endowed Fe 3 O 4 NPs with an excellent water dispersibility and colloidal stability. Moreover, APTS-coated Fe 3 O 4 NPs can be further functionalized with acetyl groups with neutral surface potential following the reaction of the surface APTS amines with acetic anhydride 21 . In present study, acetylated APTS coated Fe 3 O 4 NPs with a mean diameter of 10.5 nm were synthesized, and APTS-coated Fe 3 O 4 NPs in a powder form could be dissolved in water, PBS, or cell culture medium with good colloidal stability following storage at 4℃ for at least 1 month without obvious precipitates.…”

“…NPs synthesis 3-aminopropyltrimethoxysilane (APTS) coated Fe 3 O 4 NPs were synthesized using hydrothermal approach as described previously 21 . Brie y, 1 g FeCl 2 •4H 2 O was dissolved in 6.2 ml distilled water, then 5 ml ammonium hydroxide was added and the suspension was continuously stirred at room temperature for 10 min.…”

Biofunctionalization of magnetite nanoparticles with stevioside: effect on the size and thermal behaviour for use in hyperthermia applications

“…Magnetic nanoparticles (MNPs) have been extensively used for various biomedical applications such as hyperthermia, in vivo targeted drug delivery and MRI contrast agents [1][2][3][4]. The superparamagnetic behavior of the nanomagnets, where magnetization relaxes very fast due to thermal fluctuations has been used for these techniques.…”