Magnetic Resonance Imaging of Inducible E-Selectin Expression in Human Endothelial Cell Culture

Kang, Hye Won; Josephson, Lee; Petrovsky, Alexander; Weissleder, Ralph; Bogdanov, Alexei A.

doi:10.1021/bc0155521

Cited by 219 publications

(130 citation statements)

References 27 publications

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“…15,23,76 Using SPIO-antihuman E-selectin F(ab )2 conjugates, E-selectin was detected by MR imaging in human endothelial cell cultures, exhibiting a significant contrast enhancement over control cells. 70 Imaging of thrombi has recently been accomplished through the specific detection of both αIIb-β3, which is expressed by activated platelets, and activated coagulation factor XIII (FXIIIa), a tissue transglutaminase that crosslinks fibrin and plasmin inhibitors to form stable thrombi. 7 Activated platelets were targeted with SPIO-RGD peptide conjugates in both ex vivo and in vivo thrombus models.…”

Section: Cardiovascular Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Cardiovascular Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…There are several methods of conducting MR imaging of cells, including the use of cell pellets (14), gelatinous material including cells (15)(16)(17)(18)(19), and particular instruments for cell culture (9,20). Only two reports (9,20) have investigated the ADC values of cells using a particular cell culture device referred to as a 'Hollow fiber' device together with an experimental MRI system.…”

Section: Discussionmentioning

confidence: 99%

In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology

Matsumoto

Kuroda²,

Matsuya

et al. 2009

Oncol Rep

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Abstract. Diffusion-weighted magnetic resonance imaging (MRI) is frequently used clinically, and is available for the whole-body screening for tumors. The exact mechanism by which the apparent diffusion coefficient (ADC) value decreases in tumorous tissue remains unclear, although various theories have been proposed, including intracellular and extracellular factor theories. It is impossible to distinguish each factor in the intracellular and extracellular spaces as the source of MR signal generation by means of conventional comparison between MR images and pathological specimens. Other factors which have been reported to affect ADC include cellularity and cellular edema of human tissues, and temperature of phantoms at the time of measurement. We employed a new technique that enables cellular MR imaging using a newly developed bio-phantom containing a living culture tumor cell line, Jurkat-N1. We investigated possible reasons for observed decreases in ADC values for tumors, and we considered the contribution of both the intracellular and extracellular space to such a decrease. The ADC values of the bio-phantom increased with increasing heat exposure from 27 to 45˚C. ADC values also increased after the destruction by sonication of tumor cell membranes. ADC values decreased as cellularity increased in the bio-phantom. ADC values decreased due to cellular edema caused by a low salt concentration in the bio-phantom. Changes in pressure in the bio-phantom had no effect on the observed ADC values. We calculated both the intracellular ADC and extracellular ADC values using the ADC values, cellularity, and cellular volume of Jurkat-N1 cells in the bio-phantom. The extracellular ADC values in the bio-phantom were estimated to be lower than the ADC value of distilled water. These results indicate that not only intracellular ADC values, but also extracellular ADC values contribute to the determination of the ADC values of bio-phantoms. This is the first report to have examined the contribution of intracellular and extracellular space on the ADC values of bio-phantoms containing cultured tumor cells.

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“…Furthermore, due to the specific binding of the material to a cellular surface receptor, a receptor-mediated endocytosis process is often initiated, resulting in the internalization and intracellular accumulation of the contrast agent [44][45][46]. Active targeting has been explored for imaging a number of conditions including cancer (Figure 1) [43,[47][48][49], myocardial infarction [50], and inflamed endothelial cells [51] (possibly indicating atherosclerosis) to name a few. The topic of targeting is quite broad and we will not cover it in any more detail in this review.…”

Section: Targetingmentioning

confidence: 99%

Nanotechnology and Diagnostic Imaging: New Advances in Contrast Agent Technology

Rosen¹,

Yoffe²,

Meerasa³

2011

J Nanomedic Nanotechnol

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Medical imaging technologies allow for the rapid diagnosis and evaluation of a wide range of pathologies. In order to increase their sensitivity and utility, many imaging technologies such as CT and MRI rely on intravenously administered contrast agents. While the current generation of contrast agents has enabled rapid diagnosis, they still suffer from many undesirable drawbacks including a lack of tissue specificity and systemic toxicity issues. Through advances made in nanotechnology and materials science, researchers are now creating a new generation of contrast agents that overcome many of these challenges, and are capable of providing more sensitive and specific information. In this review, we summarize the main classes of nanotechnology-based contrast agents for each of the major imaging technologies, and highlight progress in their development as well as the challenges to be addressed. We also review the relevant biological interactions that determine the in vivo fate of these contrast agents, and describe major themes in medical nanotechnology including stealth and targeting.

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Magnetic Resonance Imaging of Inducible E-Selectin Expression in Human Endothelial Cell Culture

Cited by 219 publications

References 27 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology

Nanotechnology and Diagnostic Imaging: New Advances in Contrast Agent Technology

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