Magnetic resonance imaging of iron-oxide labeled SK-Mel 28 human melanoma cells in the chick embryo using a clinical whole body MRI scanner

Oppitz, Matthias; Pintaske, J.; Kehlbach, Rainer; Schick, Fritz; Schriek, Gernot; Busch, Christian

doi:10.1007/s10334-006-0062-y

Cited by 16 publications

(17 citation statements)

References 29 publications

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“…Anesthesia/sedation of chick embryos has received some attention over the last years, in particular as non-invasive in vivo imaging techniques become more and more available, triggering interest in suitable anesthesia protocols for commensurate applications. In the few studies conducted so far, mild cooling 7 8 , ketamine anesthesia 9 10 21 and isoflurane 11 12 received most attention. As eggs need to be windowed for our studies for planting tissue engineered constructs onto the CAM, the CAM is easily accessible for soluble anesthetics and hence medetomidine and thiopental were included into our investigations as members of different substance classes not studied so far.…”

Section: Discussionmentioning

confidence: 99%

“…For example, chick embryos were studied in ovo using MRI during development 7 8 using mild cooling conditions for sedation. Other studies applied ketamine anesthesia during MRI for characterization of embryonic tissue development 9 or in cell tracking studies in the living chick embryo 10 Also inhalant anesthetics were employed such as halothane and isoflurane delivered through the egg shell 11 12 13 . These however required more handling and specific equipment, such as sealable plastic bags to maintain the egg under anesthesia atmosphere, as well as dedicated vaporizers.…”

mentioning

confidence: 99%

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Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Waschkies

Nicholls

Buschmann

2015

Sci Rep

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Non-invasive assessment of the perfusion capacity of tissue engineered constructs grown on the chorioallantoic membrane by MRI is often hampered by motion artifacts. Therefore, we examined the suitability of three anesthetic regimes for sufficient sedation of the chick embryo. Medetomidine at a dosage of 0.3 mg/kg, was compared to thiopental at 100 mg/kg and ketamine/midazolam at 50 mg/kg and 1 mg/kg, respectively. These soluble anesthetics were applied by dropping a total volume of 0.3 mL onto the surface of the CAM. Motion was videotaped through the window of the eggshell and scored semi-quantitatively. Medetomidine performed best in terms of reduced motion; onset of anesthesia occurred within 10 minutes and for the following 30 minutes, allowing proper in vivo MRI measurements. The other regimen were not sedating deep enough (ketamine/midazolam) and not long enough (thiopental). In sum, medetomidine allows proper sedation for MRI assessment of the perfusion capacity in a tissue engineered construct placed on the CAM.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Waschkies

Nicholls

Buschmann

2015

Sci Rep

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“…These particles have a strong effect on MRI signal intensities if sufficient amount can be labeled to the target cells and retained for a long-term during the cell tracking process. [17]…”

Section: Discussionmentioning

confidence: 99%

Evaluating the effect of ultrasmall superparamagnetic iron oxide nanoparticles for a long-term magnetic cell labeling

Shanehsazzadeh¹,

Oghabian²,

Allen³

et al. 2013

J Med Phys

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In order to evaluate the long-term viability, the iron content stability, and the labeling efficiency of mammalian cells using magnetic cell labeling; dextran-coated ultrasmall superparamagnetic iron oxide (USPIOs) nanoparticles with plain surfaces having a hydrodynamic size of 25 nm were used for this study. Tests were carried out in four groups each containing 5 flasks of 5.5 × 106 AD-293 embryonic kidney cells. The cell lines were incubated for 24 h using four different iron concentrations with and without protamine sulfate (Pro), washed with phosphate-buffered saline (PBS) and centrifuged three times to remove the unbounded USPIOs. Cell viability was also verified using USPIOs. There were no significant differences in the cell viability between the control group of cells and those groups with iron uptake at the specified iron concentrations. The average iron uptake ratio compared to that of the control group was (114 ± 1). The magnetic resonance images (MRI) at post-labeling day 1 and day 21 showed (75 ± 4)% and (22 ± 5)% signal decrements compared to that of the control, respectively. The Perl's Prussian blue test showed that 98% of the cells were labeled, and the iron concentration within the media did not affect the cell iron uptake. Magnetic cellular labeling with the USPIO-Pro complex had no short or medium term (3 weeks) toxic effects on AD-293 embryonic kidney cells.

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“…Modern science provides a number of techniques for acquiring 3D information. Classical (medical) computed tomography (CT) or magnetic resonance tomography (MRT) yields a resolution down to 500 μm (Oppitz et al , 2007; Yoon et al , 2008) and is, therefore, not precise enough for most specimens in biology and palaeontology. The same holds true for surface laser scanners or mechanical surface digitizers that can usually likewise be used only for large specimens (Lyons et al , 2000; Wilhite, 2003; Tse et al , 2006).…”

Section: Introductionmentioning

confidence: 99%

Simple 3D images from fossil and Recent micromaterial using light microscopy

Haug

Maas

et al. 2009

Journal of Microscopy

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Magnetic resonance imaging of iron-oxide labeled SK-Mel 28 human melanoma cells in the chick embryo using a clinical whole body MRI scanner

Cited by 16 publications

References 29 publications

Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Evaluating the effect of ultrasmall superparamagnetic iron oxide nanoparticles for a long-term magnetic cell labeling

Simple 3D images from fossil and Recent micromaterial using light microscopy

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