Magnetic separation and characterization of keratinocyte stem cells from human gingiva

Abstract

The human gingival CD71(neg) cell fraction, separated by a magnetic system, demonstrated several characteristics of gingival keratinocyte stem cells. It is also suggested that a magnetic system may be an important tool in acquiring oral keratinocyte stem cells for research.

Epithelial Cell Rests of Malassez Contain Unique Stem Cell Populations Capable of Undergoing Epithelial–Mesenchymal Transition

“…Basal keratinocytes expressing high levels of a 6 integrin or CD49f have been shown to display keratinocyte stem cell attributes, while low expressing a 6 cells are post-mitotic differentiating keratinocytes [26,27,47]. Subsequently, oral keratinocyte stem cells have been isolated from oral mucosa using this marker [48] and also according to their relative cells size [49]. The present study has demonstrated that the enrichment of clonogenic ERM cells can be successfully achieved on the basis of integrin a 6 expression, which was less expressed or lacking on clonogenic periodontal ligament stem cells.…”

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

“…Several recent studies in the oral mucosa used morphological and clonogenic characteristics to assert that cells isolated using putative stem cell markers were indeed stem cells. 30,31,32,33 …”

High-purity Hepatic Lineage Differentiated from Dental Pulp Stem Cells in Serum-free Medium

“…In recent years, dramatic improvement in magnetic cell separation techniques has made it more popular for isolating target cells compared with the use of a flow cytometer. Magnetic separation might not affect the integrity and scrupulousness of DNA in nuclei because fluorescent dye is not used (9,10). This is a huge advantage to use magnetic separation for future regenerative medicine.…”