Magnetic stimulation modulates structural synaptic plasticity and regulates BDNF–TrkB signal pathway in cultured hippocampal neurons

Ma, Jun; Zhang, Zhanchi; Su, Yuhong; Kang, Lin; Geng, Dandan; Wang, Yanyong; Luan, Feng; Wang, Mingwei; Cui, Huixian

doi:10.1016/j.neuint.2012.11.010

Cited by 103 publications

(87 citation statements)

References 44 publications

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“…This discrepancy may be attributed to different protocols used for rTMS in addition to other parameters (intensity, area of stimulation) that may play a role. In an rTMS study in aging mice, low intensity stimulation increased the expression of synaptic proteins whereas high intensity stimulation did the opposite [12, 23]. A study in our lab also supports that rTMS at low to medium frequency and intensity is beneficial whereas that at high frequency and intensity is detrimental (reported in another paper submitted).…”

Section: Discussionsupporting

confidence: 70%

The Role of Hippocampal Structural Synaptic Plasticity in Repetitive Transcranial Magnetic Stimulation to Improve Cognitive Function in Male SAMP8 Mice

Wang

et al. 2017

Cell Physiol Biochem

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Background: Repetitive transcranial magnetic stimulation (rTMS) has been used to improve cognitive function, but the stimulation protocols are variable and the underlying mechanism is unclear. Therefore, we intend to examine whether 5Hz rTMS with 30% maximum output could improve cognitive functions in senescence-accelerated-prone mouse 8 (SAMP8) through changing synaptic plasticity. Methods: SAMP8 and senescence-accelerated-prone mouse/resistant 1 (SAMR1) (7-month old male) were randomly divided into 3 groups: SMAP8 rTMS group (P8-rTMS), SMAP8 sham-rTMS group (P8-sham), and SAMR1 sham-rTMS group (R1-sham). The P8-rTMS group was treated daily with 5Hz rTMS with 30% maximum output for 14 consecutive days, whereas the other two groups were controls without rTMS stimulation. Morris water maze (MWM) experiment was performed after rTMS or sham treatment to assess the effect of rTMS on cognitive function. Reverse transcription polymerase chain reaction and Western blot assays were used to detect the mRNA and protein expression of presynaptic Synapsin (SYN) and postsynaptic density 95 (PSD95) in the hippocampus of these mice. Results: The mean escape latency of the P8-rTMS group was significantly shorter than that of the P8-sham group. The number of platform crossings of the P8-rTMS group was significantly higher than that of the P8-sham group. rTMS significantly upregulated the protein and mRNA expression of SYN and PSD95 in the hippocampus of p8-rTMS mice compared to those of P8 sham mice. Conclusion: 5Hz rTMS with 30% maximum output enhances learning and memory in the SAMP8 mice. This improvement may be associated with the increased expression of synaptic structure proteins SYN and PSD95 in the hippocampus.

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Section: Discussionsupporting

confidence: 70%

The Role of Hippocampal Structural Synaptic Plasticity in Repetitive Transcranial Magnetic Stimulation to Improve Cognitive Function in Male SAMP8 Mice

Wang

et al. 2017

Cell Physiol Biochem

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“…A human study confirmed that after‐effects of rTMS (theta burst stimulation) are linked to the function of the NMDAR 44. Moreover, animal and behavioral studies45, 46, 47, 48, 49 provide compelling evidence that rTMS alters synaptic plasticity of hippocampal areas. Thus, rTMS may be used in order to improve hippocampal function and increase neuronal NMDAR expression.…”

Section: Discussionmentioning

confidence: 81%

Altered paired associative stimulation‐induced plasticity in NMDAR encephalitis

Volz

Finke

Harms

et al. 2016

Ann Clin Transl Neurol

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ObjectiveTo determine whether neurophysiological mechanisms indicating cortical excitability, long‐term potentiation (LTP)‐like plasticity, GABAergic and glutamatergic function are altered in patients with anti‐N‐methyl‐d‐aspartate receptor (NMDAR) encephalitis and whether they can be helpful as markers of diagnostic assessment, disease progression, and potentially therapy response.MethodsNeurophysiological characterizations of patients with NMDAR encephalitis (n = 34, mean age: 28 ± 11 years; 30 females) and age/gender‐matched healthy controls (n = 27, 28.5 ± 10 years; 25 females) were performed using transcranial magnetic stimulation‐derived protocols including resting motor threshold, recruitment curve, intracortical facilitation, short intracortical inhibition, and cortical silent period. Paired associative stimulation (PAS) was applied to assess LTP‐like mechanisms which are mediated through NMDAR. Moreover, resting state functional connectivity was determined using functional magnetic resonance imaging.Results PAS‐induced plasticity differed significantly between groups (P = 0.0056). Cortical excitability, as assessed via motor‐evoked potentials after PAS, decreased in patients, whereas it increased in controls indicating malfunctioning of NMDAR in encephalitis patients. Lower PAS‐induced plasticity significantly correlated with the modified Rankin Scale (mRS) (r = −0.41; P = 0.0031) and was correlated with lower functional connectivity within the motor network in NMDAR encephalitis patients (P < 0.001, uncorrected). Other neurophysiological parameters were not significantly different between groups. Follow‐up assessments were available in six patients and demonstrated parallel improvement of PAS‐induced plasticity and mRS.InterpretationAssessment of PAS‐induced plasticity may help to determine NMDAR dysfunction and disease severity in NMDAR encephalitis, and might even aid as a sensitive, noninvasive, and well‐tolerated “electrophysiological biomarker” to monitor therapy response in the future.Clinical Trial RegistrationClinicalTrials.gov: Identifier: NCT01865578

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“…The M H was 1.721 emu/g in 5% MNP magnetic nanocomposite, 5.691 emu/g in 10% MNP magnetic nanocomposite, and 8.063 emu/g in 20% MNP magnetic nanocomposite, respectively. The magnetic property of magnetic scaffolds was confirmed using a magnet ( Position (º2θ) 10 Morphology of magnetic membranes and magnetic scaffolds SEM was introduced to study the morphology of magnetic membranes and magnetic scaffolds. The surface of the membranes was neat, and showed almost no cracks ( Figure 6, A-D).…”

Section: Magnetic Characterizationmentioning

confidence: 99%

“…[8][9][10] Recently, magnetic composites via applied MFs have been shown to further improve the biological properties of cells. It has been shown that a magnetic composite via MF is capable of stimulating endothelial cell proliferation, promoting organization of endothelial cells into capillary-like structures, 11 facilitating organization of cardiac cells into myocardial tissue in vitro, 12 and enhancing osteogenesis for bone repair in vivo.…”

Section: Introductionmentioning

confidence: 99%

Activation of Schwann cells in vitro by magnetic nanocomposites via applied magnetic field

Liu¹,

Huang²,

Liu³

et al. 2014

IJN

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Schwann cells (SCs) are attractive seed cells in neural tissue engineering, but their application is limited by attenuated biological activities and impaired functions with aging. Therefore, it is important to explore an approach to enhance the viability and biological properties of SCs. In the present study, a magnetic composite made of magnetically responsive magnetic nanoparticles (MNPs) and a biodegradable chitosan–glycerophosphate polymer were prepared and characterized. It was further explored whether such magnetic nanocomposites via applied magnetic fields would regulate SC biological activities. The magnetization of the magnetic nanocomposite was measured by a vibrating sample magnetometer. The compositional characterization of the magnetic nanocomposite was examined by Fourier-transform infrared and X-ray diffraction. The tolerance of SCs to the magnetic fields was tested by flow-cytometry assay. The proliferation of cells was examined by a 5-ethynyl-2-deoxyuridine-labeling assay, a PrestoBlue assay, and a Live/Dead assay. Messenger ribonucleic acid of BDNF, GDNF, NT-3, and VEGF in SCs was assayed by quantitative real-time polymerase chain reaction. The amount of BDNF, GDNF, NT-3, and VEGF secreted from SCs was determined by enzyme-linked immunosorbent assay. It was found that magnetic nanocomposites containing 10% MNPs showed a cross-section diameter of 32.33±1.81 µm, porosity of 80.41%±0.72%, and magnetization of 5.691 emu/g at 8 kOe. The 10% MNP magnetic nanocomposites were able to support cell adhesion and spreading and further promote proliferation of SCs under magnetic field exposure. Interestingly, a magnetic field applied through the 10% MNP magnetic scaffold significantly increased the gene expression and protein secretion of BDNF, GDNF, NT-3, and VEGF. This work is the first stage in our understanding of how to precisely regulate the viability and biological properties of SCs in tissue-engineering grafts, which combined with additional molecular factors may lead to the development of new nerve grafts.

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Magnetic stimulation modulates structural synaptic plasticity and regulates BDNF–TrkB signal pathway in cultured hippocampal neurons

Cited by 103 publications

References 44 publications

The Role of Hippocampal Structural Synaptic Plasticity in Repetitive Transcranial Magnetic Stimulation to Improve Cognitive Function in Male SAMP8 Mice

The Role of Hippocampal Structural Synaptic Plasticity in Repetitive Transcranial Magnetic Stimulation to Improve Cognitive Function in Male SAMP8 Mice

Altered paired associative stimulation‐induced plasticity in NMDAR encephalitis

Activation of Schwann cells in vitro by magnetic nanocomposites via applied magnetic field

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