Magneto-sensitive decellularized bone matrix with or without low frequency-pulsed electromagnetic field exposure for the healing of a critical-size bone defect

Parmaksız, Mahmut; Lalegül‐Ülker, Özge; Vurat, Murat Taner; Elçin, Ayşe Eser; Elçin, Yaşar Murat

doi:10.1016/j.msec.2021.112065

Cited by 23 publications

(21 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Importantly, in vivo studies confirmed that PEMFs significantly improve bone-implant osseointegration [110,121]. Parmaksiz et al [122] transplanted the decellularized cancellous bone matrix (DBM) and DBM with incorporated magnetic iron oxide nanoparticles (MNPs) in the rat cranial defect model with or without LF-PEMF treatment. The in vivo study showed that implantation of DBM and DBM/MNPs in combination with LF-PEMF treatment promoted osteoblastic regeneration and angiogenesis.…”

Section: Pulsed Electromagnetic Fields-based Bioreactorsmentioning

confidence: 99%

Bioengineered Living Bone Grafts—A Concise Review on Bioreactors and Production Techniques In Vitro

Kazimierczak

Przekora

2022

IJMS

View full text Add to dashboard Cite

It has been observed that bone fractures carry a risk of high mortality and morbidity. The deployment of a proper bone healing method is essential to achieve the desired success. Over the years, bone tissue engineering (BTE) has appeared to be a very promising approach aimed at restoring bone defects. The main role of the BTE is to apply new, efficient, and functional bone regeneration therapy via a combination of bone scaffolds with cells and/or healing promotive factors (e.g., growth factors and bioactive agents). The modern approach involves also the production of living bone grafts in vitro by long-term culture of cell-seeded biomaterials, often with the use of bioreactors. This review presents the most recent findings concerning biomaterials, cells, and techniques used for the production of living bone grafts under in vitro conditions. Particular attention has been given to features of known bioreactor systems currently used in BTE: perfusion bioreactors, rotating bioreactors, and spinner flask bioreactors. Although bioreactor systems are still characterized by some limitations, they are excellent platforms to form bioengineered living bone grafts in vitro for bone fracture regeneration. Moreover, the review article also describes the types of biomaterials and sources of cells that can be used in BTE as well as the role of three-dimensional bioprinting and pulsed electromagnetic fields in both bone healing and BTE.

show abstract

Section: Pulsed Electromagnetic Fields-based Bioreactorsmentioning

confidence: 99%

Bioengineered Living Bone Grafts—A Concise Review on Bioreactors and Production Techniques In Vitro

Kazimierczak

Przekora

2022

IJMS

View full text Add to dashboard Cite

show abstract

“…These strategies have been clinically used to promote the healing of fractures; thus, their use can be extended to the treatment of critical bone defects. For example, Parmaksiz et al transplanted DBM and DBM containing magnetic particles to bilateral critical-size cranial defects of rats with or without PEMF exposure, and results revealed that exposed groups of DBM and DBM containing magnetic particles show improved bone regeneration and reduced fibrous formation compared with unexposed groups ( Parmaksiz et al, 2021 ). Yan et al also verified that postoperative LIPUS could promote the healing of rabbit femurs defects ( Yan et al, 2016 ).…”

Section: Applications Of Biophysical Stimuli For Bone Tissue Engineeringmentioning

confidence: 99%

Biophysical Stimuli as the Fourth Pillar of Bone Tissue Engineering

Hao

Wang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The repair of critical bone defects remains challenging worldwide. Three canonical pillars (biomaterial scaffolds, bioactive molecules, and stem cells) of bone tissue engineering have been widely used for bone regeneration in separate or combined strategies, but the delivery of bioactive molecules has several obvious drawbacks. Biophysical stimuli have great potential to become the fourth pillar of bone tissue engineering, which can be categorized into three groups depending on their physical properties: internal structural stimuli, external mechanical stimuli, and electromagnetic stimuli. In this review, distinctive biophysical stimuli coupled with their osteoinductive windows or parameters are initially presented to induce the osteogenesis of mesenchymal stem cells (MSCs). Then, osteoinductive mechanisms of biophysical transduction (a combination of mechanotransduction and electrocoupling) are reviewed to direct the osteogenic differentiation of MSCs. These mechanisms include biophysical sensing, transmission, and regulation. Furthermore, distinctive application strategies of biophysical stimuli are presented for bone tissue engineering, including predesigned biomaterials, tissue-engineered bone grafts, and postoperative biophysical stimuli loading strategies. Finally, ongoing challenges and future perspectives are discussed.

show abstract

“…The possible cytotoxic effect of DBM and the two injectable formulations developed in this work was evaluated using the MTT test, a colorimetric method that quantifies the activity of succinic dehydrogenase (SDH) [65] and is widely used as an indication of cellular mitochondrial function [66]. The results are shown in Figure 7B.…”

Section: Mtt Assaymentioning

confidence: 99%

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Medrano-David

Lopera

Londoño

et al. 2021

JFB

View full text Add to dashboard Cite

The occurrence of bone-related disorders and diseases has dramatically increased in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision. In addition, these particles are susceptible to migration to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill a bone defect. The aim of this research was to develop a DBM carrier with such viscoelastic properties in order to obtain an injectable bone substitute (IBS). The developed DBM carrier consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by an injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results showed that the developed material has great potential to be used in bone tissue regeneration.

show abstract

Magneto-sensitive decellularized bone matrix with or without low frequency-pulsed electromagnetic field exposure for the healing of a critical-size bone defect

Cited by 23 publications

References 47 publications

Bioengineered Living Bone Grafts—A Concise Review on Bioreactors and Production Techniques In Vitro

Bioengineered Living Bone Grafts—A Concise Review on Bioreactors and Production Techniques In Vitro

Biophysical Stimuli as the Fourth Pillar of Bone Tissue Engineering

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Contact Info

Product

Resources

About