Magnetoresistive Biosensors for Direct Detection of Magnetic Nanoparticle Conjugated Biomarkers on a Chip

Huang, Hao-Ting; Garu, Prabir; Li, C. H.; Chang, Wei; Chen, B. W.; Sung, Shian Ying; Lee, C. M.; Chen, J. Y.; Hsieh, Tsung-Cheng; Sheu, Wen-Jenn; Ouyang, Hao; Wang, W. C.; Chang, Ching‐Ray; Wang, C. L.; Hsu, Ming Shinn; Wei, Zung‐Hang

doi:10.1142/s2010324719400022

Cited by 33 publications

(25 citation statements)

References 100 publications

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“… 31 Second, MNPs exhibit highly stable physical properties that can be detected using low-cost instrumentation. 32 , 33 Third, MNPs are relatively inexpensive to produce. Fourth, MNPs can be manipulated by external magnetic fields providing the possibility to perform target enrichment before quantification, thereby increasing the sensitivity of the biosensor.…”

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confidence: 99%

Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements

et al. 2020

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Understanding the binding mechanism between probe-functionalized magnetic nanoparticles (MNPs) and DNA targets or amplification products thereof is essential in the optimization of magnetic biosensors for the detection of DNA. Herein, the molecular interaction forming hybrid structures upon hybridization between DNA-functionalized magnetic nanoparticles, exhibiting Brownian relaxation, and rolling circle amplification products (DNA-coils) is investigated by the use of atomic force microscopy in a liquid environment and magnetic biosensors measuring the frequency-dependent magnetic response and the frequency-dependent modulation of light transmission. This approach reveals the qualitative and quantitative correlations between the morphological features of the hybrid structures with their magnetic response. The suppression of the high-frequency peak in the magnetic response and the appearance of a new peak at lower frequencies match the formation of larger sized assemblies upon increasing the concentration of DNA-coils. Furthermore, an increase of the DNA-coil concentration induces an increase in the number of MNPs per hybrid structure. This study provides new insights into the DNA–MNP binding mechanism, and its versatility is of considerable importance for the mechanistic characterization of other DNA-nanoparticle biosensor systems.

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confidence: 99%

Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements

et al. 2020

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“… 33 Superparamagnetic nanoparticles are widely used in bioimaging and biomedical detection due to their relatively low cost, ease of functionalization, and biocompatibility. 34 Amphiphilic polymers around the magnetic nanoparticle cores offer a universal approach to extend the functionalization possibilities of the core–shell nanoparticles with biomolecules, which make these systems magnetothermally resistive sensing units. 35 Importantly, assembling these sensing units into gel structures further expand the application potential of these systems.…”

Section: Introductionmentioning

confidence: 99%

Aerogelation of Polymer-Coated Photoluminescent, Plasmonic, and Magnetic Nanoparticles for Biosensing Applications

Altenschmidt

Sánchez‐Paradinas

Lübkemann

et al. 2021

ACS Appl. Nano Mater.

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Macroscopic materials with nanoscopic properties have recently been synthesized by self-assembling defined nanoparticles to form self-supported networks, so-called aerogels. Motivated by the promising properties of this class of materials, the search for versatile routes toward the controlled assembly of presynthesized nanoparticles into such ultralight macroscopic materials has become a great interest. Overcoating procedures of colloidal nanoparticles with polymers offer versatile means to produce aerogels from nanoparticles, regardless of their size, shape, or properties while retaining their original characteristics. Herein, we report on the surface modification and assembly of various building blocks: photoluminescent nanorods, magnetic nanospheres, and plasmonic nanocubes with particle sizes between 5 and 40 nm. The polymer employed for the coating was poly(isobutylene- alt -maleic anhydride) modified with 1-dodecylamine side chains. The amphiphilic character of the polymer facilitates the stability of the nanocrystals in aqueous media. Hydrogels are prepared via triggering the colloidally stable solutions, with aqueous cations acting as linkers between the functional groups of the polymer shell. Upon supercritical drying, the hydrogels are successfully converted into macroscopic aerogels with highly porous, open structure. Due to the noninvasive preparation method, the nanoscopic properties of the building blocks are retained in the monolithic aerogels, leading to the powerful transfer of these properties to the macroscale. The open pore system, the universality of the polymer-coating strategy, and the large accessibility of the network make these gel structures promising biosensing platforms. Functionalizing the polymer shell with biomolecules opens up the possibility to utilize the nanoscopic properties of the building blocks in fluorescent probing, magnetoresistive sensing, and plasmonic-driven thermal sensing.

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“…Magnetic nanoparticles are an extremely valuable substrate for the attachment of homogeneous organic and inorganic catalysts [13]. Besides, this technique has been applied in numerous industries such as anticancer drugs supports [14], magnetic resonance imaging (MRI) [15] and, biosensors [16].…”

Section: Introductionmentioning

confidence: 99%

Enhanced biological activity of Candida rugosa lipase via immobilization on magnetic nanoparticles

et al. 2020

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Magnetic nanoparticles Fe3O4 were synthesized by the co-precipitation method. Magnetic nanoparticles were activated and modified with (3-aminopropyl) triethoxysilane (APTES) and glutaraldehyde (GA), respectively. Then, these nanoparticles were characterized by transmission electron microscope and Fourier transform infrared. Candida rugosa lipase (CRL) was successfully immobilized on GAMNPs by the chemical method via forming a covalent bond between a functional group on an enzyme protein molecule and a reactive group on the surface of solid support by chemical interaction. CRL-Fe3O4 displayed an elevate recovery rate of upward to 1348%, which was a 13-fold improvement in its free one. Compared to a free enzyme, the pH and thermal properties of the immobilized lipase were also increased. This study clearly indicates that GAMNPs could be deemed good support for the immobilization of enzymes.

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Magnetoresistive Biosensors for Direct Detection of Magnetic Nanoparticle Conjugated Biomarkers on a Chip

Cited by 33 publications

References 100 publications

Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements

Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements

Aerogelation of Polymer-Coated Photoluminescent, Plasmonic, and Magnetic Nanoparticles for Biosensing Applications

Enhanced biological activity of Candida rugosa lipase via immobilization on magnetic nanoparticles

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