Magnitude of Rotavirus A and Campylobacter jejuni infections in children with diarrhea in Twin cities of Rawalpindi and Islamabad, Pakistan

Sadiq, Asma; Bokhari, Habib; Noreen, Zobia; Asghar, Rai Muhammad; Bostan, Nazish

doi:10.1186/s12879-019-4575-1

Cited by 15 publications

(14 citation statements)

References 43 publications

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“…Among the Campylobacter -positive patients, there were more male (60%) than female (40%), which was in agreement with the results reported by Sadiq et al [ 26 ] in 2019; the study reported that the prevalence of C. jejuni was statistically higher in men than in women [ 26 ]. Moreover, our results revealed a more severe status of acute gastroenteritis in patients infected with Campylobacter than in those infected with other enteropathogens.…”

Section: Discussionsupporting

confidence: 90%

Prevalence, Laboratory Findings and Clinical Characteristics of Campylobacteriosis Agents among Hospitalized Children with Acute Gastroenteritis in Lebanon

Ghssein

Awada

Salami

et al. 2021

Pediatr Gastroenterol Hepatol Nutr

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Purpose Campylobacter species are currently the most common cause of bacterial gastroenteritis. In Lebanon, Campylobacter infection occurrence is underdiagnosed owing to the lack of specific culture and rapid test kits, particularly among children. This study aimed to evaluate the prevalence, laboratory findings, and clinical characteristics of Campylobacter infection in hospitalized children with acute gastroenteritis in South Lebanon. Methods We conducted a 6-month retrospective cohort study between January and June 2018, including 291 children aged between 1 month and 12 years, who were admitted to a tertiary healthcare center in South Lebanon. The medical files of the patients were reviewed to retrieve the required clinical information, including clinical and laboratory data. Results The prevalence of campylobacteriosis agents in pediatric patients with acute gastroenteritis is 12.02%. Patients infected with Campylobacter had more severe acute gastroenteritis than Campylobacter -negative patients and often presented with high-grade fever, diarrhea episodes more than six times per day, diarrhea lasting for more than five days, and dehydration. Indeed, children with high-grade fever (≥38.5°C) were five times more likely to test positive for Campylobacter than those with low-grade fever. In addition, the results showed a higher Vesikari score for the majority of Campylobacter -positive patients with severe acute gastroenteritis compared to a moderate profile for Campylobacter -negative patients. Conclusion The present study findings highlight that Campylobacter infection is frequent among children with acute gastroenteritis. Therefore, the detection of Campylobacter should be carried out for the diagnosis of human gastroenteritis in Lebanon, along with the detection of routine enteropathogens.

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Section: Discussionsupporting

confidence: 90%

Prevalence, Laboratory Findings and Clinical Characteristics of Campylobacteriosis Agents among Hospitalized Children with Acute Gastroenteritis in Lebanon

Ghssein

Awada

Salami

et al. 2021

Pediatr Gastroenterol Hepatol Nutr

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“…There were 1.7% of mixed infestations of A. lumbricoides and T. trichura, while 0.8% had A. lumbricoides and G. lamblia. Sadiq et al (2019) described 500 stool samples of patients with symptoms of acute diarrhea with C. jejuni accounting for 48.2% and campylobacter for 52% of all studied cases. The median age of children with both rotaviruses A (RVA) and C. jejuni infection was 6-11 months.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological studies on gastroenteritis in children in the Bannu district, Khyber Pakhtunkhwa, Pakistan

Khan

2021

J Public Health (Berl.)

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Aims Gastroenteritis is a digestive disorder among children with symptoms of abdominal cramps, diarrhoea, and vomiting. This study aimed to determine the prevalence of gastroenteritis in children in the Bannu district in 2019, and also contributed for adopting preventive measures to reduce mortality in children. Subject and methods A retrospective study was conducted to determine the prevalence of gastroenteritis in children in Bannu. The data were collected from official registers of admission maintained in the children wards in the Women and Children Hospital, Bannu. Patients with symptoms of gastroenteritis at the outpatient department were admitted to one of the children wards.Results Overall, 1456 children-897 (61.4%) males and 559 (38.6%) females-suffered from gastroenteritis during the study period. The age group ≤6 months demonstrated the highest share (37.8%), followed by >6 m ≤ 1y (35.5%), >1y ≤ 2y (15.8%), >2y ≤ 5y (7.3%), >5 ≤ 10y (3.2%), and > 10y ≤ 15y (0.4%). Overall, 89.1% of cases were from children ≤2 years old and 96.4% of cases were attributed to children ≤ 5 years of age. April demonstrated the highest percentage of prevalence of 17.7, followed by May (13.5%), November (13%), June (11.7%), September (10.4%), October (9.8%), July (9.5%), August (8.4%), March (3.4%), and February (2.6%). The age group ≤6 m was the dominant group during February through May and was replaced by the age group >6 m ≤ 1y during August through November. Conclusion Gastroenteritis showed a reduced prevalence when shifted from lower to higher age groups. Being male and age group ≤6 m showed the highest prevalence of gastroenteritis with the peak of disease in April. Further research is needed to determine the cause-based prevalence of different gastroenteritis cases in the study area.

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“…The reverse transcriptase PCR (RT-PCR) was then performed by using the commercially available Qiagen OneStep RT-PCR Kit (Qiagen, The Netherlands). The RT-PCR parameters are used as described by Sadiq and her colleagues [32] and included initial reverse transcription (at 50 C for 30 min), polymerase activation (at 95 °C for 15 min), 40 cycles of amplification (denaturation: at 94 °C for 45 s), annealing for VP4 (at 45 °C for 45 s) and annealing for VP7 (at 50 °C for 45 s), product extension (at 72 °C for 1 min), and final extension (at 72 °C for 10 min). After completion of RT-PCR, the resulting PCR products were subjected to polyacrylamide gel electrophoresis, further stained with ethidium bromide (EtBr, Sigma Aldrich), and envisioned on ultraviolet light (UV-light).…”

Section: Molecular Analysis Of Vp4 and Vp7mentioning

confidence: 99%

Seroprevalence of Anti-tTg-IgA among Symptomized Celiac Disease Patients and Their Correlation with Rotavirus Infection

Sadiq¹,

Khan

Ullah

et al. 2022

BioMed Research International

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Celiac disease (CD) is a chronic inflammatory disorder in the intestinal tract as a response to the use of gluten in genetically predisposed individuals. It is a worldwide problem, with a high prevalence rate in North America. This is a descriptive cross-sectional study involving 1090 samples collected from different hospitals of Rawalpindi and Islamabad, Pakistan, from January 2019 to December 2019. In this study, 1090 blood samples screened for seroprevalence of anti-tTG antibodies in CD symptomatic patients via ELISA (enzyme-linked immunosorbent assay). 1090 fecal samples from the same CD patients were collected and tested for the presence of rotavirus (RV) via ELISA and RT-PCR. Of the 1090 patients tested for seroprevalence of anti-tTG antibodies, 112/1090 (10.3%) were found to be positive. Of the 112 anti-tTG-positive patients, 78/112 (70%) were positive for RV via ELISA and 74/112 (66.1%) were RV positive via RT-PCR. A statistically significant association was reported between rotavirus infection and celiac disease ( p ˂ 0.05 ). Anti-tTG antibodies were higher in age group 6 (12-18 years) patients (18.4%) and at minimum in age group 3 (1-3 years) patients (4.8%). However, there was a statistically insignificant relationship between group age and CD prevalence ( p > 0.05 ). The highest CD prevalence was noted during winter season (19.6%) and the lowest (3.0%) during fall/autumn. Our study findings demonstrate that Pakistan has a high prevalence of CD compared to other studies. Further studies in the fields of environmental risk factors and treatment with more advanced serological and histopathological studies are needed in the future.

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Magnitude of Rotavirus A and Campylobacter jejuni infections in children with diarrhea in Twin cities of Rawalpindi and Islamabad, Pakistan

Cited by 15 publications

References 43 publications

Prevalence, Laboratory Findings and Clinical Characteristics of Campylobacteriosis Agents among Hospitalized Children with Acute Gastroenteritis in Lebanon

Prevalence, Laboratory Findings and Clinical Characteristics of Campylobacteriosis Agents among Hospitalized Children with Acute Gastroenteritis in Lebanon

Epidemiological studies on gastroenteritis in children in the Bannu district, Khyber Pakhtunkhwa, Pakistan

Seroprevalence of Anti-tTg-IgA among Symptomized Celiac Disease Patients and Their Correlation with Rotavirus Infection

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