Maintenance and in Vitro Conservation of Potatoes

Dimitrova, Dessislava; Marcheva, M. P.

doi:10.17660/actahortic.2009.830.7

Cited by 4 publications

(2 citation statements)

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“…The use of growth inhibitors or retardants can induce slow growth in vitro. Alar (also known as Daminozide) and ABA, for instance, were tested at 50 mg L -1 on 2 cultivars maintained for 12 months at low temperature (8-10 °C) and 16 h photoperiod, showing a slight decrease in vitality (80%) during the recovery (Dimitrova and Marcheva 2009). Significant interaction was detected between the ancymidol, a growth retardant, and other factors, such as sucrose, temperature and genotype, on survival and regrowth of shoots in post-conservation.…”

Section: Potato (Solanum Tuberosum)mentioning

confidence: 99%

Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation

2020

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Today, application of in vitro culture by means of slow growth storage of shoot cultures and cryopreservation of organs, tissues and cells in liquid nitrogen presents a remarkable strategic tool to support medium-and long-term conservation of plant genetic resources. Over the last 30 years, considerable progresses have been made in the development of both methods that are currently considered as ex situ conservation strategies, complementary to traditional seed banks and in-field clonal collections. Efficient protocols were developed for the conservation of a large number of crops, including strategically-important vegetables, such as garlic, artichoke, asparagus, cassava, Jerusalem artichoke, mint, potato, sweet potato, chicory, taro, thyme and yam. As a consequence, more than 45,000 accessions of vegetable crops are maintained in 22 genetic resources conservation centers (biobanks), located in 16 countries and 6 continents (Europe, Asia, Africa, Oceania, North and South America). Approximately 4/5 of these accessions are maintained in vitro by means of slow growth storage of shoot cultures, but cryopreservation is also constantly growing, with almost 8300 vegetable accessions being stored in liquid nitrogen at -196 °C.

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Section: Potato (Solanum Tuberosum)mentioning

confidence: 99%

Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation

2020

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“…The best results are achieved with the use of growth inhibitors, where 12-month storage is reached with preserved viability of explants up to 80% (Dimitrova, 2009).…”

Section: Total 17mentioning

confidence: 99%

Plant Genetic Resources as a part of the biodiversity

Krasteva¹,

Uzundzhalieva²,

Ruseva³

2012

AGR

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SummaryThe preservation of plant biodiversity of Bulgarian flora is the main priority in scientific activities of IPGR -Sadovo. It is a part of the National Programme on Plant Genetic resources (PGR). Plant accessions in the base collections are evaluated according to the standards of FAO and European programme for PGR. The scientific programme for conservation of germplasm under controlled conditions in the gene bank is prepared according to the FAO requirements. The national collection of PGR in Sadovo includes crops and crop wild relatives-new varieties, selection lines, old varieties, local populations, mutants and wild species. The collection is divided into 7 main groups: cereals, grain legumes, industrial crops, vegetable crops, forage crops and medical and ornamental plants. The scientific work is carried out in 6 directions: enrichment with new geneplasm; evaluation of PGR; maintenance of PGR; conservation of PGR; documentation of PGR; use of PGR.

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