Maintenance of phenotype and function of cryopreserved bone-derived cells

Wang, Shaoyi; Zhao, Jun; Zhang, Wenjie; Ye, Dongxia; Yu, Wenwen; Zhu, Chao; Zhang, Xiuli; Sun, Xiaojuan; Chen, Yang; Jiang, Xinquan; Zhang, Zhiyuan

doi:10.1016/j.biomaterials.2011.01.020

Cited by 13 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The frozen bone chips were thawed and incubated with standard Dulbecco's modified Eagle's medium containing 10% (v/v) fetal bovine serum (Hyclone, Logan, UT, USA) after a prescribed 3 months of cryopreservation. The cell culture method was performed in accordance with a previous report . The medium was replaced every 3 to 4 days until cell density reached 70% to 80% confluence.…”

Section: Methodsmentioning

confidence: 99%

“…The cell culture method was performed in accordance with a previous report. 17 The medium was replaced every 3 to 4 days until cell density reached 70% to 80% confluence. After the first passage, the following three supplements were added: 100 nM dexamethasone, 0.05 mM ascorbic acid 2-phosphate, and 10 mM betaglycerophosphate.…”

Section: Cryopreservation and Cell Culturementioning

confidence: 99%

“…In previous studies, we isolated living CBOs from banked tissue and demonstrated that CBOs could be acquired easily, with a good proliferative activity and an osteogenic phenotype. Our group has shown that CBOs could be used as a new cell source for tissue engineering and regenerative medicine because they meet generally accepted standards as seeded cells . However, whether these cells can be applied in the reconstruction of segmental mandibular defects in a large animal model is still largely unknown.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Wang

Zhao

Zhang

et al. 2013

Clin Implant Dent Rel Res

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Cryopreservation and Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Wang

Zhao

Zhang

et al. 2013

Clin Implant Dent Rel Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Processes such as irradiation and freeze drying are used to decrease risks, but these procedures also eliminate the cellular component, resulting in reduced osteoinductivity [8]. Moreover, allogeneic bone has decreased revascularization and a higher resorption rate [2], resulting in a lower rate of new bone tissue formation as compared to autologous bone [8, 9]. Another alternative method is synthetic prostheses such as hydroxyapatite, beta-Tricalcium phosphate, and calcium phosphate cements.…”

Section: Introductionmentioning

confidence: 99%

Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells

et al. 2013

View full text Add to dashboard Cite

Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, very few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable tissue-engineered bone composed of mesenchymal stem cells (MSCs) and platelet rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out pilot trial cases in patients with long-term follow up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months was significantly higher than the pre-operation baseline (P <0.001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during long term follow-up. Injectable tissue-engineered bone restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering.

show abstract

“…The undecalcified specimens of 6 nude mice (4 specimens from each group) were processed following these steps: (1) dehydration in a graded series of ethanol from 75% to 100%; (2) embedding in polymethylmethacrylate (PMMA); (3) sectioning (150 mm thick) using a microtome (Leica, Hamburg, Germany); (4) polishing the sections to a final thickness of approximately 40 mm; and (5) staining the sections with Van Gieson's picrofuchsin [25]. Lastly, under an inverted microscope, sections were observed for new bone formation and remnant scaffolds.…”

Section: Methodsmentioning

confidence: 99%

The Bone-Forming Effects of HIF-1α-Transduced BMSCs Promote Osseointegration with Dental Implant in Canine Mandible

Zou

Zhang

et al. 2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The presence of insufficient bone volume remains a major clinical problem for dental implant placement to restore the oral function. Gene-transduced stem cells provide a promising approach for inducing bone regeneration and enhancing osseointegration in dental implants with tissue engineering technology. Our previous studies have demonstrated that the hypoxia-inducible factor-1α (HIF-1α) promotes osteogenesis in rat bone mesenchymal stem cells (BMSCs). In this study, the function of HIF-1α was validated for the first time in a preclinical large animal canine model in term of its ability to promote new bone formation in defects around implants as well as the osseointegration between tissue-engineered bone and dental implants. A lentiviral vector was constructed with the constitutively active form of HIF-1α (cHIF). The ectopic bone formation was evaluated in nude mice. The therapeutic potential of HIF-1α-overexpressing canine BMSCs in bone repair was evaluated in mesi-implant defects of immediate post-extraction implants in the canine mandible. HIF-1α mediated canine BMSCs significantly promoted new bone formation both subcutaneously and in mesi-implant defects, including increased bone volume, bone mineral density, trabecular thickness, and trabecular bone volume fraction. Furthermore, osseointegration was significantly enhanced by HIF-1α-overexpressing canine BMSCs. This study provides an important experimental evidence in a preclinical large animal model concerning to the potential applications of HIF-1α in promoting new bone formation as well as the osseointegration of immediate implantation for oral function restoration.

show abstract

Maintenance of phenotype and function of cryopreserved bone-derived cells

Cited by 13 publications

References 30 publications

Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells

The Bone-Forming Effects of HIF-1α-Transduced BMSCs Promote Osseointegration with Dental Implant in Canine Mandible

Contact Info

Product

Resources

About