Maintenance of plasma-derived proteins at much lower concentrations in the uterine lumen of the rabbit: a kinetic study of passage

Kulangara, Abraham C.

doi:10.1530/jrf.0.0460189

Cited by 11 publications

(2 citation statements)

References 23 publications

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“…Previous studies have indicated that about 90 % of the macromolecules in the uterine fluids of nonpregnant females are derived from plasma in the rabbit (Kulangara, 1976) and the rat (Ringler, 1961), but the present results do not confirm this for the rat. This study defines qualitative and quantitative differences between the uterine luminal proteins and serum of the rat and shows that the endocrine state of the females is associated with marked changes in the macromolecular composition of the uterine fluids.…”

Section: Discussioncontrasting

confidence: 99%

Qualitative and quantitative examination of the proteins of rat uterine luminal fluid during pro-oestrus and pregnancy and comparison with those of serum

Surani¹

1977

Reproduction

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Rat serum and uterine luminal proteins at two distinct endocrinological stages were analyzed electrophoretically. The quantitative relationships between the major macromolecular fractions were calculated from the densitometer scans. At pro-oestrus there was a marked increase in the total luminal proteins which was probably not due to transudation of serum into the lumen because of the presence of large amounts of two proteins, of approximate mol. wt 125,000 and 130,000, which were not detected in serum. Quantitative estimates of proteins of different molecular weight fractions in the fluids showed that there were considerable differences between the three fluids, indicating that the hormones do not merely induce a free passage of serum proteins into the lumen. On Day 5 of pregnancy there was a considerable lower amount of protein in the uterine lumen and qualitatively these proteins differed from those in serum and the uterine lumen at pro-oestrus.

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Section: Discussioncontrasting

confidence: 99%

Qualitative and quantitative examination of the proteins of rat uterine luminal fluid during pro-oestrus and pregnancy and comparison with those of serum

Surani¹

1977

Reproduction

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“…Comparison of in situ hybridization patterns at these stages (Figs 4A and 5A) suggests that only the more superficial epithelial cells contain GP42 message in the PSP uterus. We are not aware of other reports indicating possible blastocyst-induced alterations in rabbit endo¬ metrial glycoprotein secretion during the periimplantation period, although conceptus effects on the epithelial glycocalyx (Anderson & Hoffman 1984) and stromal-vascular prostaglandin synthesis (Hoffman et al 1984) Kulangara 1976). That GP42 is not of vascular origin was suggested by the demonstration of immunoreactive GP42 in the serum-free medium from epithelial cells isolated on day 4 PSP and cultured for four days (Hoffman et al 1996).…”

Section: Discussionmentioning

confidence: 96%

Specific expression of haptoglobin mRNA in implantation-stage rabbit uterine epithelium

Olson

Winfrey²,

Matrisian³

et al. 1997

Journal of Endocrinology

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A glycoprotein, termed GP42, was previously identified in uterine fluid obtained from peri-implantation-stage rabbits. N-terminus amino acid sequencing of purified GP42 demonstrated identity through the first 13 amino acids with the beta subunit of liver haptoglobin. The present study was undertaken to determine if GP42 is indeed identical to haptoglobin and, if so, to determine whether it is expressed in the uterus as opposed to being present as a transudate from plasma. Reverse transcription-PCR amplification of poly(A)+ RNA prepared from implantation-stage rabbit endometrium with GP42- and haptoglobin-specific primers yielded a predicted 667 bp cDNA product. Sequence analysis of the cloned cDNA confirmed the identity of GP42 with beta-haptoglobin. Northern blot analysis demonstrated the specific expression of haptoglobin mRNA in the peri-implantation-stage endometrium and the absence of its expression in the estrous or day 4 pseudopregnant endometrium. Non-isotopic in situ hybridization revealed that the haptoglobin mRNA was restricted to the epithelium lining the luminal surface and mucosal folds of day 6(3/4) pregnant or pseudo-pregnant uteri and that no haptoglobin mRNA was detectable in the epithelium of the deep glands or cells of the stroma or myometrium. Similarly, in situ hybridization revealed no expression of haptoglobin mRNA in any cell types of the estrous uterus. These data establish the identity of GP42 with beta-haptoglobin and demonstrate that it is expressed in a stage-specific manner just prior to implantation, correlating with uterine receptivity to blastocyst implantation. Endometrial GP42 mRNA expression is not dependent on the presence of blastocysts.

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Bovine serum albumin, sperm motility, and the “dilution effect”

1982

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Epididymal spermatozoa from rabbit and ram were washed either once or twice using an efficient washing procedure and were then diluted in various media to a final concentration of approximately 1.4 x 10(7) cells/ml and incubated at 30 degrees C for up to 12 hours. Bovine serum albumin (BSA), either untreated or defatted, was found to be better than polyvinylpyrrolidone, ovalbumin, or alpha-lactalbumin, both at stimulating and maintaining motility levels and at reducing the tendency of the washed spermatozoa to stick to glass. BSA was effective in all media tested, being independent of Ca2+, PO4(3-), HCO3-, and ionic strength). BSA has a reversible stimulatory effect on motility. If BSA was added to sperm suspensions 3 1/2 hours after they had been washed and diluted in protein-free medium, motility was stimulated to levels not significantly lower than those observed in samples that had been washed and diluted in the presence of BSA. However, samples washed into BSA and then washed free of it behaved essentially as though they had never been in contact with protein. The motility, survival, and response to BSA of twice-washed spermatozoa were the same as those of once-washed spermatozoa, showing that epididymal plasma factors are not required for survival in vitro. It was concluded that dilution is not essentially detrimental to rabbit and ram spermatozoa. However, severe dilution of semen may result in levels of male reproductive tract fluids insufficient either to stimulate motility or to prevent sticking of motile cells to container surfaces. Few motile spermatozoa are recovered from samples of such diluted semen.

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Maintenance of plasma-derived proteins at much lower concentrations in the uterine lumen of the rabbit: a kinetic study of passage

Cited by 11 publications

References 23 publications

Qualitative and quantitative examination of the proteins of rat uterine luminal fluid during pro-oestrus and pregnancy and comparison with those of serum

Qualitative and quantitative examination of the proteins of rat uterine luminal fluid during pro-oestrus and pregnancy and comparison with those of serum

Specific expression of haptoglobin mRNA in implantation-stage rabbit uterine epithelium

Bovine serum albumin, sperm motility, and the “dilution effect”

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