Dutch rabbits were injected with human or bovine serum albumin or human gamma globulin at 19, 24, and 28 days of gestation. Fetal serum, stomach contents, and exocoel, amniotic, and allantoic fluids were collected at intervals from 1 to 48 hr later and titrated with specific antisera. The presence of injected protein in fetal samples was confirmed by electrophoresis and absorption of rabbit protein, followed by the precipitin test. The three nonantibody proteins were detected in fetal serum and exocoel fluid after day 19 and in all fetal compartments after day 22, as soon as 1 hr after injection into the mother and after a maternal dose as low as 10 mg/kg., About 1 mg was found in the entire fetus 24 hr after a maternal dose of 62.5 mg/kg. The proteins passed from maternal circulation to uterine lumen, to exocoel, to allantois and amnion, and from amnion to stomach. They passed from mother to fetal circulation via the vessels of the yolk sac splanchnopleure and not through the allantoidean placenta.

Uterine lumina of rabbits were rinsed in situ with BSA solution. Uterine fluid volume and luminal protein concentration were calculated from radial immunodiffusion and biuret tests of the rinse. The procedure was checked for three sources of error and confirmed by double rinses. Non-pregnant and lactating rabbits have nearly equal amounts of protein per horn, but different volumes of fluid. With pregnancy, increase of volume occurs without proportionate increase of protein. Peak protein concentrations occur in pregnant and pseudopregnant rabbits at 5 days p.c. Volume and protein concentration of the luminal fluid appear to be sensitive indicators of the hormonal and reproductive status of the uterus.

207mor cells, it is speculated that immunization has a lethal rather than a static effect on transplanted tumors.Discussion. McKee et aZ.(3) were unable to induce immunity by treatment with Ehrlich's ascites cells that had been killed by desiccation, freezing and thawing, mechanical grinding, and supersonic waves. The possibility exists that the physical-chemical changes of tumor antigens induced by in vitro x-irradiation are extensive enough to make the antigens less homologous and more antigenic for the mouse without destroying antigenic specificity. Indirect evidence for this is manifested by recent observations made in this laboratory. The agglutinins against normal Ehrlich's tumor cells are more readily produced and demonstrated in sera of animals that have been exposed to higher irradiation doses .Summary. Pre-immunization with irradiated Ehrlich's ascites tumor cells protected mice against subsequent ip transplants of viable cells. Immunization procedures started one day after sc challenge with the tumor, significantly reduced mortality. Such post-immunization did not inhibit the rapid multiplication that follows ip transplantation of the tumor. The evidence indicated that the immunization procedures employed resulted in a lethal rather than a static effect on transplanted tumor cells.

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