Volume and Protein Concentration of Rabbit Uterine Fluid

Kulangara, Abraham C.

doi:10.1530/jrf.0.0280419

Cited by 16 publications

(8 citation statements)

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“…Ligation and cannulation (Wales, 1973) have been shown to produce slightly or distinctly higher protein content. Direct aspiration and luminal rinsing com¬ bined with volume estimation (Kulangara, 1972) seem to be reliable methods to obtain luminal concentration. The disparity between protein levels in fluid from Dutch Belted (14-5 mg/ml) and New Zealand White (4-8 mg/ml) rabbits may be a breed difference or due to greater variability of the earlier data.…”

Section: Discussionmentioning

confidence: 99%

Maintenance of plasma-derived proteins at much lower concentrations in the uterine lumen of the rabbit: a kinetic study of passage

Kulangara¹

1976

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Human serum albumin (HSA) and human gamma globulin (HGG) in serum and uterine fluid of nonpregnant rabbits at various times after an i.v. injection (100 mg/kg) were measured by a radial immunodiffusion test using specific antisera. The HSA concentration in uterine fluid rose to a peak at 12 hr when it was 11% of the serum concentration and then declined, whereas HGG reached a peak at 18 hr (3\m=.\2% of serum level) and decreased thereafter. The HSA passed 2\ m=1/ 2\ times faster than HGG, but both proteins equilibrated with uterine fluid in about 12-18 hr.Steady state levels of HSA and HGG indicated that uterine fluid:serum ratios were 1:10 and 1:20, respectively. Similar ratios were found for total protein and rabbit serum albumin (1:10) and rabbit gamma globulin (1:20). Therefore, except when there is a local immune response, the uterine lumen contains only about 5% of the serum antibody concentration.Available data in the mouse, rat and dog also indicate disparity between serum and uterine fluid protein levels.

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Section: Discussionmentioning

confidence: 99%

Maintenance of plasma-derived proteins at much lower concentrations in the uterine lumen of the rabbit: a kinetic study of passage

Kulangara¹

1976

Reproduction

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“…For instance, during blastocyst implantation, the volume and composition of uterine fluid have been reported to undergo significant changes to facilitate blastocyst attachment and embedding [45]. Disturbance of the fluid microenvironment, i.e., by bacterial infection or in disease condition such as cystic fibrosis (CF), results in implantation failure and infertility [44,4648].…”

Section: Ion Channels/transporters and Their Crosstalk With The Micromentioning

confidence: 99%

Ion channels/transporters as epigenetic regulators? —a microRNA perspective

Jiang

Zhang

Chan

2012

Sci. China Life Sci.

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MicroRNA (miRNA) alterations in response to changes in an extracellular microenvironment have been observed and considered as one of the major mechanisms for epigenetic modifications of the cell. While enormous efforts have been made in the understanding of the role of miRNAs in regulating cellular responses to the microenvironment, the mechanistic insight into how extracellular signals can be transduced into miRNA alterations in cells is still lacking. Interestingly, recent studies have shown that ion channels/transporters, which are known to conduct or transport ions across the cell membrane, also exhibit changes in levels of expression and activities in response to changes of extracellular microenvironment. More importantly, alterations in expression and function of ion channels/transporters have been shown to result in changes in miRNAs that are known to change in response to alteration of the microenvironment. In this review, we aim to summarize the recent data demonstrating the ability of ion channels/transporters to transduce extracellular signals into miRNA changes and propose a potential link between cells and their microenvironment through ion channels/transporters. At the same time, we hope to provide new insights into epigenetic regulatory mechanisms underlying a number of physiological and pathological processes, including embryo development and cancer metastasis. ion channel, miRNAs, epigenetic, microenvironment

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“…Oviducal and uterine secretions change continuously in volume and in the viscosity and concentration of their constituents (Lutwak-Mann, 1966;Kulangara, 1972 Lutwak-Mann (1966 and Kirchner (1969) (Schwick, 1965;Kunitake, Nakamura, Wells & Moyer, 1965;Beier, 1967Beier, , 1968aBeier, , b, 1972Kirchner, 1969;Krishnan & Daniel, 1968 The predominant fraction in the post-albumin region is the pregnancy-specific rabbit uterine protein which has been called uteroglobin (Beier, 1966(Beier, , 1967 (Dies, 1972). The molecular weight of uteroglobin originally calculated to be 30,000 (apparently identical to the blastokinin fraction, described independently by Krishnan & Daniel (1967) (Beier, 1968a, b) and in the human by Moghissi (1970).…”

Section: Oviducal and Uterine Compartmentsmentioning

confidence: 99%