Maize seed cryo-storage modifies chlorophyll, carotenoid, protein, aldehyde and phenolics levels during early stages of germination

Arguedas, Melissa; Gómez, Daviel; Hernández, Lázaro; Engelmann, Florent; Garramone, Raffaele; Cejas, Inaudis; Yabor, Lourdes; Martínez-Montero, Marcos Edel; Lorenzo, José Carlos

doi:10.1007/s11738-018-2695-7

Cited by 14 publications

(7 citation statements)

References 28 publications

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“…Therefore effects of seed storage on early seedling establishment need to be understood to produce high-quality planting stock. However, studies researching seedling physiology after seeds storage are still scarce in the literature, they confirm a possible effect on seedling quality [49][50][51]. The effect of storage conditions on seedling performance is here discussed in terms of searching early signs of deterioration in stored orthodox seeds.…”

Section: Discussionmentioning

confidence: 97%

Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus

et al. 2020

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Seeds stored in controlled conditions in gene banks, faster or slower lose their viability. The effects of seed moisture content levels (ca. 5, 8, 11%) combined with storage temperatures (-3˚,-18˚,-196˚C) were investigated in terms of the description of seeds defined as orthodox under oxidative stress after seed storage, during germination, and initial seedling growth. Hydrogen peroxide (H 2 O 2), thiobarbituric acid reactive substances (TBARS) and ascorbate (Asc) were analyzed in relation to seed germinability and seedlings emergence in three species: Malus sylvestris L., Prunus avium L. and Prunus padus L. The effect of seed storage conditions on H 2 O 2 levels appeared in germinated seeds after the third year of storage in each species. The H 2 O 2 levels were negatively correlated with the germination and seedling emergence of P. avium seeds after three years of storage under all examined combinations. The emergence of P. padus seedlings was not linked to any of the stress markers tested. The P. padus seed biochemical traits were least altered by storage conditions, and the seeds produced tolerant seedlings of relatively high levels of H 2 O 2 and TBARS. To cope with different H 2 O 2 levels, TBARS levels, and Asc levels in seeds of three species varying storage conditions different molecular responses, i.e. repairing mechanisms, were applied during stratification to compensate for the storage conditions and, as a result, seeds remained viable and seedlings were successfully established.

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Section: Discussionmentioning

confidence: 97%

Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus

et al. 2020

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“…To our knowledge, studies examining seedling quality after storage are still rare. Recent publications assessing this subject confirm a possible effect on seedling quality both in annual plants (Marshall and Lewis 2004;Arguedas et al 2018) and in woody species (Benamirouche et al 2018).…”

Section: Introductionmentioning

confidence: 95%

Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants

Wawrzyniak

Michalak

Chmielarz

2020

Annals of Forest Science

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Key message Malus sylvestris(L.) Mill.,Pyrus communis(L.),Sorbus aucuparia(L.),Prunus avium(L.),Prunus padus(L.), andCornus sanguinea(L.) are related, co-occurring species producingorthodoxseeds. However, we observed differences in their response to storage conditions, such as storage at different seed moisture contents (5%, 8%, and 11%) and/or temperatures (− 3 °C, − 18 °C, and − 196 °C). Severe desiccation to ca. 5% of MC negatively affected seeds ofM. sylvestris. Seeds ofP. aviumwere sensitive to storage in LN or at − 18 °C.S. aucupariaseeds are best stored at − 3 °C, whereasC. sanguineaseeds tolerate desiccation and storage in LN. In general, species with deeper physiological dormancy (S. aucuparia,P. padus, andC. sanguinea) tended to be more tolerant to desiccation and low temperatures. For all species, storage conditions did not affect seedling growth. Context Wild fruit woody species face many threats such as genetic loss, population fragmentation, and alien species; thus, their genetic variability should be preserved. Aims To examine the effect of storage conditions on seed viability and the initial growth of seedlings of six European wild fruit species: Malus sylvestris (L.) Mill., Pyrus communis (L.), Sorbus aucuparia (L.), Prunus avium (L.), Prunus padus (L.), and Cornus sanguinea (L.). Methods Seeds were desiccated to three different levels of moisture content (ca. 5, 8, or 11%) and stored at three different temperatures (− 3°, − 18°, or − 196 °C; liquid nitrogen, LN) for up to 3 years. Germination and seedling emergence tests were performed as well as measurement of seedling growth. Results Desiccation of M. sylvestris seeds from 10.7 to 4.9% significantly lowered germination from 91 to 77% and seedling emergence from 88 to 74%. In P. avium, LN storage significantly inhibited seedling emergence, both in the laboratory and the greenhouse, but did not affect total seed germination. In P. communis, P. padus, and C. sanguinea, neither germination nor seedling emergence was affected by seed storage conditions. There were small or no differences in stem height and root collar diameter in the first year of seedling growth of stored seeds. Conclusion Species with deeper physiological dormancy (S. aucuparia, P. padus, C. sanguinea) tended to be more tolerant of various storage conditions. Seeds of P. padus and C. sanguinea can be stored long term at − 18 °C or in LN at 5–8% MC without losing viability. M. sylvestris and P. avium seeds are sensitive to desiccation below 6% MC or low temperature of storage at − 18 °C or − 196 °C, respectively. We observed that storage conditions had significant influence on germination and seedling emergence but had no effect on seedling growth after the first growing season.

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Section: Field Performancementioning

confidence: 99%

“…No significant differences in seed germinations were obtained between cryopreserved and non-cryopreserved (control) seeds in Phaseolus vulgaris [ 97 , 131 ], Solanum lycopersicum [ 132 ], and Zea mays [ 133 ]. However, reduced seed germinations were reported in the cryopreserved seeds of Zea mays and Glycine max [ 133 , 134 ]. In the study of cryopreservation of Solanum lycopersicum seeds, Zevallos et al [ 135 ] reported that cryopreservation increased the germination percentage of cryopreserved seeds at day 5 of germination, albeit with no significant differences at day 7.…”

Section: Field Performancementioning

confidence: 99%

“…The morphology of the seedlings developed from cryopreserved seeds were similar to those from the control in Phaseolus vulgaris [ 97 , 131 ], Solanum lycopersicum [ 135 ], Zea mays [ 134 ], Glycine max [ 134 ], and Hibiscus sabdariffa [ 137 ]. Seedling growth, measured by the fresh weight of roots, stem, and leaves, was markedly delayed in cryopreserved seeds of Zea mays [ 133 ]. Seedling growth, including plant height and fresh and dry mass, was greater in seedlings recovered from cryopreserved seeds than in those from the control during the four-week growth [ 136 ].…”

Section: Field Performancementioning

confidence: 99%

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Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Shukla

Ren

et al. 2021

Plants

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Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

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Maize seed cryo-storage modifies chlorophyll, carotenoid, protein, aldehyde and phenolics levels during early stages of germination

Cited by 14 publications

References 28 publications

Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus

Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus

Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

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