Making Progress in Plant Proteomics for Improved Food Safety

Righetti, Pier Giorgio; Fasoli, Elisa; D’Amato, Alfonsina; Boschetti, Egisto

doi:10.1016/b978-0-444-62650-9.00006-3

Cited by 6 publications

(4 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main characteristics of each method are summarized in Table 1. These enrichment method proposals have been described for animal proteomes [17], plant proteomes [18,19] and even recombinant therapeutic proteins when attempting to detect contaminating proteins from the host cells. In this domain, dedicated methods are described.…”

Section: Current Methods For Low-abundance Protein Enrichmentmentioning

confidence: 99%

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Boschetti¹,

Righetti

2023

IJMS

Self Cite

View full text Add to dashboard Cite

The discovery of low- and very low-abundance proteins in medical applications is considered a key success factor in various important domains. To reach this category of proteins, it is essential to adopt procedures consisting of the selective enrichment of species that are present at extremely low concentrations. In the past few years pathways towards this objective have been proposed. In this review, a general landscape of the enrichment technology situation is made first with the presentation and the use of combinatorial peptide libraries. Then, a description of this peculiar technology for the identification of early-stage biomarkers for well-known pathologies with concrete examples is given. In another field of medical applications, the determination of host cell protein traces potentially present in recombinant therapeutic proteins, such as antibodies, is discussed along with their potentially deleterious effects on the health of patients on the one hand, and on the stability of these biodrugs on the other hand. Various additional applications of medical interest are disclosed for biological fluids investigations where the target proteins are present at very low concentrations (e.g., protein allergens).

show abstract

Section: Current Methods For Low-abundance Protein Enrichmentmentioning

confidence: 99%

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Boschetti¹,

Righetti

2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…These biomarkers could become essential to guarantee the safety and authenticity of foods, ensuring rapid action in a possible outbreak event. A fast and effective protocol is imperative in the current market where products travel long distances [ 70 ]. MALDI-TOF-MS is a fast and effective technique for the identification of foodborne pathogens.…”

Section: Benefits Of Proteomics In the Production Of Cereals And Vegetable Cropsmentioning

confidence: 99%

Proteomic Advances in Cereal and Vegetable Crops

et al. 2021

View full text Add to dashboard Cite

The importance of vegetables in human nutrition, such as cereals, which in many cases represent the main source of daily energy for humans, added to the impact that the incessant increase in demographic pressure has on the demand for these plant foods, entails the search for new technologies that can alleviate this pressure on markets while reducing the carbon footprint of related activities. Plant proteomics arises as a response to these problems, and through research and the application of new technologies, it attempts to enhance areas of food science that are fundamental for the optimization of processes. This review aims to present the different approaches and tools of proteomics in the investigation of new methods for the development of vegetable crops. In the last two decades, different studies in the control of the quality of crops have reported very interesting results that can help us to verify parameters as important as food safety, the authenticity of the products, or the increase in the yield by early detection of diseases. A strategic plan that encourages the incorporation of these new methods into the industry will be essential to promote the use of proteomics and all the advantages it offers in the optimization of processes and the solution of problems.

show abstract

“…Many examples of applications have been reported, such as the extension of the list of proteins present in various proteomes of animals, plants, microbial origin, , and cell culture supernatants . Similarly the discovery of expression modification of certain proteins of low abundance upon biological disorders (see following sections), the evidence of foreign unexpected proteins in food and beverage preparations, the discovery of novel allergens, and the alteration of protein expression during the embryogenesis development have been described.…”

Section: Limits Of the Current Use Of Solid-phase Combinatorial Pepti...mentioning

confidence: 99%

Combinatorial Peptide Ligand Libraries as a “Trojan Horse” in Deep Discovery Proteomics

et al. 2014

Self Cite

View full text Add to dashboard Cite

A review. The present review covers the latest developments of the combinatorial peptide ligand library (CPLL) methodol., a very useful tool for detection of low-abundance proteins in proteomes of any origin. Originally available as a library of millions of hexa-peptides, all terminating with a free amino group, it was further modified via succinylation to carry a terminal carboxyl, for a larger capture of species. Operating CPLLs at 3 different pH values also allows a better capture, since the ionic surface charge is modulated on both the baits and the ligand proteins. Elution of captured species in boiling 2-4% SDS also permits quant. recovery of bound material, whereas any other eluant is largely insufficient for this purpose. Finally, by performing the capture either at very low ionic strength (thus favoring ionic interactions) or in presence of lyotropic salts (up to 1 M, to force hydrophobic interactions), while maintaining the other two types of capture, namely at acidic and alk. pH values, one can use the CPLL technol. as a multi-dimensional tool for max. coverage of proteomes

show abstract

Making Progress in Plant Proteomics for Improved Food Safety

Cited by 6 publications

References 87 publications

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Proteomic Advances in Cereal and Vegetable Crops

Combinatorial Peptide Ligand Libraries as a “Trojan Horse” in Deep Discovery Proteomics

Contact Info

Product

Resources

About