Making Sense of Lymphoma Diagnostics in Small Animal Patients

Burkhard, Mary Jo; Bienzle, Dorothee

doi:10.1016/j.cll.2015.05.008

Cited by 11 publications

(13 citation statements)

References 19 publications

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“…Histopathologic evaluation of intestinal biopsies remains an important diagnostic tool to differentiate IBD and intestinal lymphoma, but the latter might be a result of chronic lymphocytic‐plasmacytic inflammation, which is the most common type of chronic intestinal inflammation . Whether dogs diagnosed with chronic lymphocytic‐plasmacytic or mixed inflammation developed intestinal lymphoma later on was not evaluated in the present study (i.e, postmortem examinations and adjunctive techniques such as immunohistochemistry, flow cytometry, and PCR for antigen receptor rearrangements were not performed), and thus underlying intestinal lymphoma could have been missed, which is a limiting factor of the present study.…”

Section: Discussionmentioning

confidence: 98%

Chronic Diarrhea in Dogs – Retrospective Study in 136 Cases

Volkmann

Steiner

Fosgate

et al. 2017

Veterinary Internal Medicne

126

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BackgroundChronic diarrhea (CD) is common in dogs, and information on frequency and distribution of primary and secondary causes is lacking.ObjectivesTo evaluate underlying causes and predictors of outcome in dogs with CD.AnimalsOne hundred and thirty‐six client‐owned dogs with CD (≥3 weeks duration).MethodsRetrospective review of medical records (Small Animal Clinic, Freie Universität Berlin, Germany, 09/2009‐07/2011). Quantification of final diagnoses and comparison of clinical aspects including disease severity and clinicopathological abnormalities among dogs with clinical remission (either complete [gastrointestinal signs absent] or partial [clinical improvement of gastrointestinal signs and reduced episodes with shortened duration]), and those without recovery.ResultsNinety percent of dogs were diagnosed with a primary enteropathy: inflammatory (71%; of those 66% dietary responsive, 23% idiopathic, 11% antibiotic responsive), infectious (13%), neoplastic (4%), and in one dog each mechanical disease or systemic vasculitis. Secondary causes were diagnosed in 10% of dogs: exocrine pancreatic (6%), endocrine (2%), and in one dog each hepatic, renal, and cardiac disease. In total, 87% of dogs had clinical remission, whereas 13% died or did not respond to treatment: Lack of recovery was frequently recorded for dogs with primary inflammatory (idiopathic) or neoplastic disease and was significantly associated with increased disease severity scores (P = .005), anemia (hematocrit < 40%, P < .001), severe hypoalbuminemia (serum albumin <2.0 g/dL, P = .008), and severe hypocobalaminemia (serum cobalamin concentration <200 pg/mL, P = .006).Conclusions and clinical importanceInflammatory enteropathies and particularly those of dietary origin were the most common causes of CD in dogs. Findings support the usefulness of hematocrit, and serum albumin and cobalamin concentration as prognostic markers in dogs with CD.

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Section: Discussionmentioning

confidence: 98%

Chronic Diarrhea in Dogs – Retrospective Study in 136 Cases

Volkmann

Steiner

Fosgate

et al. 2017

Veterinary Internal Medicne

126

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“…Equally, a PARR negative result does not completely rule out lymphoma. In cats, currently available protocols detect 60 to 65% of neoplastic lymphocyte samples (Moore et al , Burkhard & Bienzle ). Furthermore, corticosteroid administration or formalin fixation may result in excessive lymphocyte DNA fragmentation, leading to false negative PARR results.…”

Section: Discussionmentioning

confidence: 99%

Differentiating feline inflammatory bowel disease from alimentary lymphoma in duodenal endoscopic biopsies

Sabattini

Bottero

Turba³

et al. 2016

J of Small Animal Practice

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Corresponding author email: giuliano.bettini@unibo.itO BJECTIVES : This study aimed to evaluate the agreement between microscopic and molecular testing for differentiating feline intestinal bowel disease and small cell alimentary lymphoma in duodenal endoscopic biopsies. M ETHODS : Four different diagnostic methods (cytology, histology, immunohistochemistry and clonality)were sequentially applied to 77 cases of feline chronic enteropathies. The agreement between the different diagnostic methods was calculated and survival data were obtained to assess the most reliable method for predicting outcome.R ESULTS : Seventy-seven cases were included in the study. On multivariate survival analysis, only the clonality-based diagnosis of lymphoma was significantly associated with poor survival, with a risk of enteropathy-related death 2·8 times higher. By comparing the other tests with clonality, specificity was high (87 to 97%), whereas sensitivity was 36·8% for cytology, 39·5% for histology, 63·2% for immunohistochemistry, resulting in an overall accuracy of 62·3, 68·8 and 80·5%, respectively.

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“…Dogs were diagnosed with multicentric nodal lymphoma by cytological assessment of lymph node aspirates [37] at the Ontario Veterinary College Mona Campbell Centre for Animal Cancer, University of Guelph. Dogs who had not received prior treatment other than a single injection of prednisone were eligible for this study ( n = 41); prednisone treatment status was known for only 31 dogs (Untreated n = 26, Treated n = 5).…”

Section: Methodsmentioning

confidence: 99%

RNA disruption indicates CHOP therapy efficacy in canine lymphoma

Parissenti

Pritzker²,

Guo

et al. 2019

BMC Vet Res

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BackgroundAssessment of the efficacy of a multi-agent chemotherapy protocol in which cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) are administered in canine lymphoma is generally performed by physical measurement of lymph node diameter. However, no consistent correlation has been made with prognostic indicators and the length or absence of clinical remission based on lymph node size. RNA disruption measured mid-therapy has been correlated with increased disease-free survival in recent studies of human cancer and was assessed in this study of canine lymphoma patients. Fine needle aspirate samples were taken before treatment and at weeks 3, 6, and 11 of CHOP therapy. RNA was isolated from these samples and assessed using an Agilent Bioanalyzer. RNA disruption assay (RDA) analysis was performed on the data from the resulting electropherograms.ResultsAn increased RNA disruption index (RDI) score was significantly associated with improved progression-free survival.ConclusionsPredicting the risk of early relapse during chemotherapy could benefit veterinary patients by reducing ineffective treatment and could allow veterinary oncologists to switch earlier to a more effective drug regimen.

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Making Sense of Lymphoma Diagnostics in Small Animal Patients

Cited by 11 publications

References 19 publications

Chronic Diarrhea in Dogs – Retrospective Study in 136 Cases

Chronic Diarrhea in Dogs – Retrospective Study in 136 Cases

Differentiating feline inflammatory bowel disease from alimentary lymphoma in duodenal endoscopic biopsies

RNA disruption indicates CHOP therapy efficacy in canine lymphoma

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