Male golden hamster in male reproductive toxicology testing: Assessment of protective activity of selenium in acute cadmium intoxication

Wlodarczyk, Bogdan J.; Biernacki, B.; Minta, Maria; Kozaczynski, W.; Juszkiewicz, T

doi:10.1007/bf00197977

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…The present study pointed suppression of relative testicular weight in cadmium treated groups (T1 and T2) with subsequent degeneration and vaculation of spermatogenic cells (fig 2,3). Such results were in agreement with (23) in their study on golden hamster. The results also was in agreement with Albeuroti (24), where different concentrations of cdcl2 (75,100,150) cause significant decrease in testicular weight.…”

Section: Discussionsupporting

confidence: 93%

Effect of Different Concentrations of Cadmium Chloride on Some Structural Changes of Testes in Adult Male Rabbits

Alhaddad¹,

Al-Okaily²,

Hussein³

2008

Bas.J.Vet.Res.

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This present study was designed to investigate the effects of two levels of cadmium chloride (30 and 40 part per billion (ppb)) in drinking water on body weight, relative weight of testes and epididymus (head, body, tail), diameter and thickness of seminiferous tubules. Eighteen mature male rabbits were randomly divided into three equal groups; the control group (GI) were given ordinary tap water and animals in the first treated group (GII) were received 30 part per billion (ppb) of cadmium chloride in drinking water; while animals in the second treated group(GIII) were given 40 ppb of cadmium chloride in drinking water. Weight of animals were taken at the pre-treated period and every three weeks during the treated period. At the end of experiment the animals were killed, testis and epididymus were taken in order to measuring their weight. Samples testis were taken for histological study and for measuring the diameter of the seminiferous tubules and the thickness of the cells lining their. The results showed a significant increase (p<0.05) in the mean body weight of animals in the groups including the control (GI).The results also pointed to occurrence of a significant decrease (p<0.05) in the relative weight of testis and epididymus of both cadmium treated groups. Besides a significant decrease (p<0.05) in the mean of diameter the seminiferous tubules and the thickness of the cells lining them were occur after exposure to cadmium chloride. Concerning the histological study of the testis the results showed presence of acute necrotic changes modified by vaculation of cytoplasm of sertolli cells with decrease in number of spermatogenic cells, GIII group were more affected than GII group. On conclusion, cadmium chloride in drinking water (30 and 40 ppb) caused functional change in spermatogenesis in adult male rabbits.

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Section: Discussionsupporting

confidence: 93%

Effect of Different Concentrations of Cadmium Chloride on Some Structural Changes of Testes in Adult Male Rabbits

Alhaddad¹,

Al-Okaily²,

Hussein³

2008

Bas.J.Vet.Res.

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“…Cd has a long biological half-life (15-20 years) and accumulates over time within the blood, kidneys, liver, and reproductive organs (Henson and Chedrese, 2004). Chronic exposure to Cd has been shown to cause reproductive impairment in male mammals, including azoospermia in hamsters (Wlodarczyk et al, 1995), failure of spermiation and low sperm production in rats (Hew et al, 1993;NTP, 1995), and abnormal sperm head morphology in mice (Mukherjee et al, 1988). A most recent single dose (sc) of Cd chloride treatment study in rat found that Cd-induced apoptosis at low doses of Cd (0.13 and 0.15 mg/100 g body weight [BW]) significantly reduced serum testosterone (T) level at doses of 0.20 and 0.3 mg/100 g BW (Sen Gupta et al, 2004).…”

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confidence: 99%

Cadmium-induced Activation of Stress Signaling Pathways, Disruption of Ubiquitin-dependent Protein Degradation and Apoptosis in Primary Rat Sertoli Cell-Gonocyte Cocultures

Yu¹,

Hong²,

Faustman³

2008

Toxicological Sciences

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Cadmium (Cd) is a ubiquitous environmental pollutant that has been associated with male reproductive toxicity in both humans and animal models. The underlying mechanism of this response, however, is still uncharacterized. To address this issue, we employed a recently developed and optimized three-dimensional primary Sertoli cell-gonocyte coculture system and examined the time- and dose-dependent effects of Cd on morphological alterations, cell viability, activation of stress signaling pathway proteins, and the disruption of the ubiquitin proteasome system (UPS). Our results demonstrated that Cd exposure lead to time- and dose-dependent morphological changes that are associated with the induction of apoptosis. In response to Cd, we also saw a disruption of the UPS as evaluated through the accumulation of high-molecular weight polyubiquitinated proteins (HMW-polyUb) as well as alterations in proteasome activity. Robust activation of cellular stress response, measured through the increased phosphorylation of stress-activated protein kinase/c-jun N-terminal kinase and p38, paralleled the accumulation of HMW-polyUb. In addition, p53, a key regulatory protein, was upregulated and underwent increased ubiquitination in response to Cd. To further characterize the role of the UPS in Cd cellular response, we compared the above changes with two classic proteasomal inhibitors, lactacystin, and MG132. The stress response and the accumulation of HWM-polyUb induced by Cd were consistent with the response seen with MG132 but not with lactacystin. In addition, Cd treatment resulted in a dose- and time-dependent effect on proteasome activity, but the overall Cd-induced proteasomal inhibition was unique as compared to MG132 and lactacystin. Taken together, our studies further characterize Cd-induced in vitro testicular toxicity and highlight the potential role of the UPS in this response.

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“…They have numerous biological functions at both the molecular and cellular levels because they are involved in the synthesis of nucleic acids and coenzyme activities and in cell division, cellular immunity, and membrane stability (Č ihá k, 1979;Ahokas et al, 1980;Li et al, 1995). Chmielnicka et al (1983a,b), Belmonte et al (1989), Mishima et al (1995), and Wlodarczyk et al (1995) have reported the protective effect of zinc and selenium against damage to cells caused by cadmium. Heavy metals induce synthesis of metallothionein (MT), a lowmolecular protein, in hepatocytes (Margeli et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Distribution of exogenous heavy metals in the hepatocytes of calves: A morphometric study

Horký

Lauschová

Illek

et al. 2002

Microscopy Res & Technique

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The objective of this study was to evaluate the presence of heavy metals in the hepatocytes of the animals fed a cadmium-supplemented diet and also receiving zinc and/or selenium in the injection form. The experiment involved four groups of calves (6-8, both sexes) receiving the heavy metals in various combinations for 95 days. Electron micrographs of liver cells were prepared and statistically evaluated using Student's t-test. A modified morphometric apparatus was used for morphometric examination. Exogenous cadmium showed marked accumulation in the hepatocytes. If, however, the cadmium diet was combined with zinc or selenium administration the amount of the reduction product was much lower.

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Male golden hamster in male reproductive toxicology testing: Assessment of protective activity of selenium in acute cadmium intoxication

Cited by 9 publications

References 7 publications

Effect of Different Concentrations of Cadmium Chloride on Some Structural Changes of Testes in Adult Male Rabbits

Effect of Different Concentrations of Cadmium Chloride on Some Structural Changes of Testes in Adult Male Rabbits

Cadmium-induced Activation of Stress Signaling Pathways, Disruption of Ubiquitin-dependent Protein Degradation and Apoptosis in Primary Rat Sertoli Cell-Gonocyte Cocultures

Distribution of exogenous heavy metals in the hepatocytes of calves: A morphometric study

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