“…The application of the zona-free hamster oocyte assay has been used to assess the fertility of men (Freeman et al, 2001; ) and domestic animals (Cormier et al, 1997;De los Reyes et al, 2009;Hewitt & England, 1997;Maxwell et al, 1996;). The OPT is a less time-consuming technique than in vitro fertilization (IVF) test, because oocytes can be immatured, and after evaluation are not further subjected to development.…”