Male Reproductive System

The effect of neural network architecture on virtual H&E staining: Systematic assessment of histological feasibility

“…Seminal vesicles are surrounded by a thin layer of stroma-like connective tissue, lined with branched tall columnar epithelium and filled with intensely eosinophilic secretion. 30 The epithelial tissue and secretion are well reproduced in the virtual staining, while the connective tissue lining is frequently missing the elongated nuclei ( Figure 6 B), similar to the prostate tissue results ( Figure 3 ). The spleen is a nuclei-dense organ that is grossly divided into two zones: the erythrocyte-rich red pulp and the basophilic white pulp, 33 both of which can be observed in the virtually stained spleen ( Figure 6 C).…”

“…The epididymis comprises epididymal ducts, which are lined with thin epithelium and the lumen is filled with mature spermatozoa. 30 The epithelial structures as well as the lumen are represented well in the virtually stained tissue, although there is occasional fading of epithelial structures and fusion of overrepresented nuclei ( Figure 6 F).…”

“…The glomeruli, which are largely irregular in shape, can be distinguished but their more nuclei-dense areas were less well reproduced. The testis is composed primarily of convoluted seminiferous tubules, 30 the structure of which can be observed clearly in the virtually stained images ( Figure 6 E). The tubules of the testis contain the maturing spermatogenic cells, which are basophilic in nature, 30 and although most of them are visible in the virtually stained images, some have not been reproduced ( Figure 6 E).…”

“…The testis is composed primarily of convoluted seminiferous tubules, 30 the structure of which can be observed clearly in the virtually stained images ( Figure 6 E). The tubules of the testis contain the maturing spermatogenic cells, which are basophilic in nature, 30 and although most of them are visible in the virtually stained images, some have not been reproduced ( Figure 6 E). The epididymis comprises epididymal ducts, which are lined with thin epithelium and the lumen is filled with mature spermatozoa.…”

“…The glands are surrounded by stroma consisting of connective tissue and smooth muscle in addition to the occasional adipose tissue, blood vessels, capillaries, and peripheral nerves. 30 In the three different virtual staining versions, all tissue components and types were readily detectable. Epithelial and adipose tissue stainings were reproduced particularly well ( Figure 3 ).…”