The effects of indole‐3‐acetic acid (IAA) and p‐chlorophenoxyisobutyric acid (PCIB) on rates of abscission layer formation and abscission were investigated. The primary leaves of Phaseolus vulgaris were used as test material. Treatment at the distal end of one petiole of the pair from debladed primary leaves with 1% IAA inhibited the abscission of that petiole and accelerated the abscission of its opposite untreated partner. PCIB applied simultaneously with IAA counteracted the accelerating effect of IAA on the opposite untreated petiole. This influence increased with increasing concentrations of PCIB. Anatomical studies revealed that PCIB, although it counteracted the effect of IAA on the rate of abscission, had no effect on abscission layer formation. In other words abscission layer formation takes place under the influence of the auxin despite the presence of the antiauxin. The centripetal sequence of abscission layer formation was found in all cases.