Malignancy and Primary Sclerosing Cholangitis: Cholangiocarcinoma, Hepatocellular Carcinoma, and Gallbladder Carcinoma

Muething, Larissa; BurtonJr., James R.

doi:10.1007/978-3-319-40908-5_2

Cited by 5 publications

(2 citation statements)

References 64 publications

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“…Its incidence has increased from 7th to 5th in the bowel tumors at the end of the 20th century (1, 2). The incidence of gallbladder cancer has increased significantly, and it is on the rise (3). The early stage of the disease is very similar to the symptoms of cholecystitis, so early diagnosis is more difficult (4).…”

Section: Introductionmentioning

confidence: 99%

The Effect and Mechanism of Vascular Endothelial Growth Fac-tor (VEGF) on Tumor Angiogenesis in Gallbladder Carcinoma

Xu¹,

Li²,

Jiang³

et al. 2019

ijph

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Background: To investigate the effect of vascular endothelial growth factor (VEGF) on tumor angiogenesis in gallbladder carcinoma. Methods: Fifty one patients with gallbladder carcinoma were enrolled as observation group. Thirty healthy people were included as control group. Chemically synthesized siRNA sequences targeting VEGF was transfected with VEGF-siRNA. A blank group (group B), a negative control group (transfected with independent sequence, group C), and an inhibition group (transfected with VEGF siRNA, group D) were established. Physiological saline was set as group A. The expression of VEGF was detected by qRT-PCR. The expression of VEGF protein was detected by Western blot. MVD was used to measure microvessel density. CCK-8, Transwell and flow cytometry were used to detect cell proliferation, invasion and apoptosis. Results: The tumor volume of nude mice and VEGF mRNA expression in group D was significantly smaller than that in group B and C (P<0.05). The MVD density in group B and C was significantly higher than that in group D (P<0.01). The proliferation of cells was detected from the 3rd day, and the proliferation of cells in the blank and negative control groups was faster than that of the inhibition group (P<0.05). The apoptosis rate of the blank group and the negative control group was lower than that of the inhibition group (P<0.001). Conclusion: VEGF is highly expressed in serum of patients with cholangiocarcinoma, it promotes angiogenesis, proliferation and invasion of gallbladder cancer cells, and inhibits apoptosis of tumor cells.

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Section: Introductionmentioning

confidence: 99%

The Effect and Mechanism of Vascular Endothelial Growth Fac-tor (VEGF) on Tumor Angiogenesis in Gallbladder Carcinoma

Xu¹,

Li²,

Jiang³

et al. 2019

ijph

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“…Recent study reported a prevalence of 4.03 PSC cases per 100,000 subjects and an incidence of 0.41 PSC per 100,000 person-years in a Caucasian population [2]. Additionally, up to 80% of PSC patients simultaneously suffer inflammatory bowel disease (IBD) [3] and an increased incidence of malignancy, such as intrahepatic cholangiocarcinoma, hepatobiliary cancer, and colorectal cancer [4][5][6][7]. Liver transplantation is the most definitive way for the treatment of PSC as there is no other better proven therapy of PSC to date.…”

Section: Introductionmentioning

confidence: 99%

Gene Set Enrichment Analysis Detected Immune Cell-Related Pathways Associated with Primary Sclerosing Cholangitis

Luo

Liu

Hou

et al. 2022

BioMed Research International

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Aim. To explore various immune cell-related causal pathways for primary sclerosing cholangitis (PSC). Methods. Immune cell-related pathway association study was conducted via integrative analysis of PSC GWAS summary and five immune cell-related eQTL datasets. The GWAS summary data of PSC was driven from 4,796 PSC cases and 19,955 healthy controls. The eQTL datasets of CD4+ T cells, CD8+ T cells, B cells, natural killer cells (NK), monocytes, and peripheral blood cells (PB) were collected from recently eQTL study. The PSC GWAS summary dataset was first aligned with eQTL datasets of six blood cells to obtain the GWAS summary data at overlapped eQTL loci, separately. For each type of cell, the obtained PSC GWAS summary dataset of eQTLs was subjected to pathway enrichment analysis. 853 biological pathways from Kyoto Encyclopedia of Genes and Genomes, BioCarta, and Reactome pathway databases were analyzed. Results. We identified 36 pathways for B cells, 33 pathways for CD4+ T cells, 28 pathways for CD8+ T cells, 33 pathways for monocytes (MN), 35 pathways for NK cells, and 33 for PB cells (all empirical P values < 5.0 × 10 − 5 ). Comparing the pathway analysis results detected 25 pathways shared by five immune cells, such as KEGG_CELL_ADHESION_MOLECULES_CAMS ( P value < 5.0 × 10 − 5 ) and REACTOME_MHC_CLASS_II_ANTIGEN_ PRESENTATION ( P value < 5.0 × 10 − 5 ). Several cell-specific pathways were also identified, including BIOCARTA_INFLAM_PATHWAY ( P value < 5 × 10 − 5 ) for B cell. Conclusion. Our study holds potential to identify novel candidate causal pathways and provides clues for revealing the complex genetic mechanism of PSC.

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Cholangiocarcinoma: Epidemiology, Pathogenesis, and Prognosis

Namn

Bucobo

2021

Hepato-Pancreato-Biliary Malignancies

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Malignancy and Primary Sclerosing Cholangitis: Cholangiocarcinoma, Hepatocellular Carcinoma, and Gallbladder Carcinoma

Cited by 5 publications

References 64 publications

The Effect and Mechanism of Vascular Endothelial Growth Fac-tor (VEGF) on Tumor Angiogenesis in Gallbladder Carcinoma

The Effect and Mechanism of Vascular Endothelial Growth Fac-tor (VEGF) on Tumor Angiogenesis in Gallbladder Carcinoma

Gene Set Enrichment Analysis Detected Immune Cell-Related Pathways Associated with Primary Sclerosing Cholangitis

Cholangiocarcinoma: Epidemiology, Pathogenesis, and Prognosis

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