Malignant histiocytosis. Complete remission in two pediatric patients

Simon, Julius H.; Tebbi, Cameron K.; Freeman, Arnold I.; Green, Daniel M.; Brecher, Martin L.; Barcos, Maurice

doi:10.1002/1097-0142(19870501)59:9<1566::aid-cncr2820590906>3.0.co;2-z

Cited by 4 publications

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References 19 publications

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“…Therefore, all therapeutic interventions were directed at the management of malignant histiocytosis. However, despite encouraging recent results using doxorubicin-and cyclophosphamide-containing regimens in this disorder [14,15], our patient's disease was refractory to all therapies and pursued a fulminant course. This has been the uniform outcome in previously reported patients with hematologic disorders and mediastinal germ cell tumors.…”

Section: Discussionmentioning

confidence: 66%

The unique association of malignant histiocytosis and a primary gonadal germ cell tumor

Margolin

Traweek

1992

Med. Pediatr. Oncol.

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Previous reports of the association between hematologic malignancies and germ cell tumors have been limited to patients with nonseminomatous tumors, exclusively of mediastinal origin. Among the various hematologic disorders, a large proportion have involved histiocytic tumors, either acute monocytic leukemia or malignant histiocytosis. We now report the first case of simultaneously occurring malignant histiocytosis and testicular embryonal carcinoma. The patient, an 18-year-old male, presented with hepatosplenomegaly due to malignant histiocytosis and was found on further evaluation to have a stage I testicular cancer consisting of teratocarcinoma with endodermal sinus elements. Despite aggressive chemotherapy, the patient died of malignant histiocytosis 6 months after the original diagnosis. The autopsy revealed widespread organ involvement with malignant histiocytosis and no evidence of residual germ cell tumor. This case demonstrates that germ cell tumors associated with hematologic malignancy do not arise solely in extragonadal sites.

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Section: Discussionmentioning

confidence: 66%

The unique association of malignant histiocytosis and a primary gonadal germ cell tumor

Margolin

Traweek

1992

Med. Pediatr. Oncol.

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Malignant Histiocytic Disorders in Children

Bucsky

Egeler

1998

Hematology/Oncology Clinics of North America

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Photosensitized destruction of human bladder carcinoma cells treated with chlorin e6-conjugated microspheres.

Bachor

Shea

Gillies

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

111

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A photosensitizer conjugate, chlorin e6 (Ce6) covalently bound to 1-uim-diameter polystyrene microspheres, has been investigated in the photodynamic destruction of MGH-U1 human bladder carcinoma cells in vitro. The microspheres were taken up avidly by the carcinoma cells; confocal laser scanning fluorescence microscopy showed them to be localized in the cytoplasm, apparently within lysosomes, visualized by labeling with acridine orange. In contrast, fluorescence of unconjugated Ce6 was present within most cellular membranes. Use of Ce6-microsphere conjugates led to a 20- collected by centrifugation at 1000 rpm for 10 min, and the fluorescence of the supernatants was measured (model Fluorolog 2; Spex Industries, Edison, NJ). Cells not exposed to either form of Ce6, and cell-free dishes incubated in the presence of plain medium, unconjugated Ce6, or Ce6-microspheres served as controls. Standard solutions containing known concentrations of unconjugated Ce6 and Ce6-microspheres in DMF and unconjugated free Ce6 in medium were prepared and their fluorescence emission spectra (obtained with excitation at 406 nm) were recorded from 600 to 850 nm. After background and instrument correction for wavelength sensitivity, the integrated fluorescence of each standard solution was measured and a calibration curve relating integrated fluorescence to Ce6 concentration was determined. The integrated fluorescence from the supernatant of each cellular sample was then measured and the Ce6 concentration of the sample was ascertained from the calibration curve.Singlet Oxygen Generation. 02(1Ad) production was measured by trapping with histidine to form an endoperoxide; the change in absorbance at 440 nm of p-nitrosodimethylaniline as a consequence of reaction with the histidine endoperoxide was used to quantify the photosensitized generation of 02(1Ag) (28). One-milliliter aliquots of Ce6 solution or Ce6-microsphere suspension containing 10 ,uM histidine and 10 ytMp-nitrosodimethylaniline in 1.5-cm-diameter dishes were either kept in the dark or irradiated at 659 nm with 0.2-5 J/cm2 and studied by absorbance spectroscopy from 300 to 700 nm. The relative quantum yield Of 02('Ag) generation was calculated after correction for the molar extinction coefficient at 659 nm for unconjugated and bound Ce6 (21,400 and 25,000 liter'mol-1cm-1, respectively). RESULTS Absorption Spectra. In DMF both unconjugated Ce6 and Ce6-microspheres had a Soret band maximum at 404 nm and a Q-band maximum at 664 nm. In DPBS the unconjugated Ce6 had a Soret band maximum at 404 nm and a Q-band maximum at 655 nm; the corresponding maxima for Ce6-microspheres in DPBS were 408 nm and 665 nm (Fig. 1) Serial optical sectioning by confocal laser scanning microscopy showed that --90%o of the microspheres present in the dishes after rinsing were taken up intracellularly and resided within the cytoplasm (Fig. 2A). The number of microspheres per cell varied from 0 to 50 and averaged about 20. Fluorescence was strictly colocalized with the microspheres (Fig. 2B) in a ...

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Malignant histiocytosis. Complete remission in two pediatric patients

Cited by 4 publications

References 19 publications

The unique association of malignant histiocytosis and a primary gonadal germ cell tumor

The unique association of malignant histiocytosis and a primary gonadal germ cell tumor

Malignant Histiocytic Disorders in Children

Photosensitized destruction of human bladder carcinoma cells treated with chlorin e6-conjugated microspheres.

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