Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163

Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8

“…Following this, the two RRs phosphorylate and attach to plnEF, which encodes the bacteriocin structural genes. Subsequently, the bacteriocin is secreted from the cell via the ABC-transport system (Zhao et al, 2021).…”

A Novel Class IIb Bacteriocin-Plantaricin EmF Effectively Inhibits Listeria monocytogenes and Extends the Shelf Life of Beef in Combination with Chitosan

“…5,6 Bacteriocins, are generally recognized as safe (GRAS) food preservatives, especially those that came from Lactiplantibacillus plantarum with biodegradable properties, no drug resistance, and low hemolytic activity. 5,7 In recent years, more and more research studies have reported that bacteriocins could contribute to prevent the contamination of meat, milk, vegetables, and fruit via inactivation of foodborne pathogenic microorganisms and avoid food spoilage. 4,5,8 For example, bacteriocin BM1300 was used to prevent the growth of Escherichia coli and Staphylococcus aureus in beef.…”

“…Bacteriocins, are generally recognized as safe (GRAS) food preservatives, especially those that came from Lactiplantibacillus plantarum with biodegradable properties, no drug resistance, and low hemolytic activity. , In recent years, more and more research studies have reported that bacteriocins could contribute to prevent the contamination of meat, milk, vegetables, and fruit via inactivation of food-borne pathogenic microorganisms and avoid food spoilage. ,, For example, bacteriocin BM1300 was used to prevent the growth of Escherichia coli and Staphylococcus aureus in beef . Pentocin 31-1, lacticin 481, pentocin MS1/MS2, and nisin were selected to control Listeria monocytogenes in pork meat, cheese, fresh-cut fruit, and milk, respectively. − Nevertheless, to date, only one bacteriocin, nisin, has been approved by the Food and Drug Administration, which has been commercially developed as a kind of biological preservative .…”

TetR-Type Regulator Lp_2642 Positively Regulates Plantaricin EF Production Based on Genome-Wide Transcriptome Sequencing of Lactiplantibacillus plantarum 163

“…Plantaricin EF is a two-peptide bacteriocin produced by Lactiplantibacillus plantarum that exerts an antibacterial effect on both Gram-positive and Gram-negative bacteria. , It is generally recognized as a safe food bio-preservative owing to its low hemolytic activity and biodegradable characteristics. , Therefore, plantaricin EF has been used to replace chemical preservatives and is considered to be a natural barrier to control foodborne pathogenic microorganisms and spoilage microorganisms, which is in great demand in the agricultural and food industries. , …”

“…It is well known that plantaricin EF is a ribosome-synthesized two-peptide bacteriocin whose biosynthesis, secretion, and processing are regulated by a pln locus that contains several operons. , In the pln locus, the operon plnEFI codes for the two-peptide bacteriocin EF and a cognate immune protein (PlnI) . The operon plnABCD codes for a quorum-sensing system (QSS), containing a histidine protein kinase PlnB, two response regulators PlnC and PlnD, and an autoinducing peptide PlnA. , The operon plnGHSTUVW encodes an ABC transport system that is used to secrete and process PlnA and plantaricin EF. , When extracellular PlnA exceeds a critical threshold, it activates PlnB on the membrane, and intracellular PlnC and PlnD are phosphorylated by the activated PlnB. Then, the phosphorylated PlnC and PlnD synergistically regulate the genes plnA and plnEF to synthesize the precursor of PlnA and plantaricin EF, respectively, which are processed to a mature PlnA and plantaricin EF and secreted from the cells by the ABC transport system .…”