Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides

Bi, Changfen; Liang, Yulu; Shen, Lijin; Tian, Shanshan; Zhang, Kai; Li, Yiliang; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1021/acsomega.7b01788

Cited by 34 publications

(10 citation statements)

References 42 publications

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“…The peaks at 1,312, 1,335 and 1,366 cm −1 belong to the C-N bond in the amino acid which has been disappeared in the Ca-Gly-Maltose nanocarrier. Compared with the spectrum of Ca-Gly, the new broad peak in the wavenumber region of 2,964–3,448 cm −1 (the carboxyl group) and an enhanced peak at 1,557 and 1,591 cm −1 (the amide group) and a peak at 1,410 cm −1 (for OH of the carboxylic acid group) in the spectrum of Ca-Gly-COOH revealed the successful modification of carboxyl groups on the surface of Ca-Gly ( Bi et al, 2018 ).…”

Section: Resultsmentioning

confidence: 96%

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Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv

Mirzaeinia

Zeinali

Budiša

et al. 2022

Front. Bioeng. Biotechnol.

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Biological metal-organic frameworks (BioMOFs) are hybrid compounds in which metal nodes are linked to biocompatible organic ligands and have potential for medical application. Herein, we developed a novel BioMOF modified with an anti-VEGFR1 scFv antibody (D16F7 scFv). Our BioMOF is co-loaded with a combination of an anticancer compound and a lipid-lowering drug to simultaneously suppress the proliferation, growth rate and metastases of cancer cells in cell culture model system. In particular, Prodigiosin (PG) and Simvastatin (SIM) were co-loaded into the newly synthesized Ca-Gly BioMOF nanoparticles coated with maltose and functionalized with a recombinant maltose binding protein-scFv fragment of anti-VEGFR1 (Ca-Gly-Maltose-D16F7). The nanoformulation, termed PG + SIM-NP-D16F7, has been shown to have strong active targeting behavior towards VEGFR1-overexpresing cancer cells. Moreover, the co-delivery of PG and SIM not only effectively inhibits the proliferation of cancer cells, but also prevents their invasion and metastasis. The PG + SIM-NP-D16F7 nanocarrier exhibited stronger cytotoxic and anti-metastatic effects compared to mono-treatment of free drugs and drug-loaded nanoparticles. Smart co-delivery of PG and SIM on BioMOF nanoparticles had synergistic effects on growth inhibition and prevented cancer cell metastasis. The present nanoplatform can be introduced as a promising tool for chemotherapy compared with mono-treatment and/or non-targeted formulations.

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Section: Resultsmentioning

confidence: 96%

“…2.5 g succinic anhydride was added to the solution of 500 mg Ca-Gly NPs in 120 ml tetrahydrofuran. The dispersion solution was refluxed for 12 h. The obtained Ca-Gly-COOH NPs were washed several times with deionized water and ethanol and dried in an oven ( Bi et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv

Mirzaeinia

Zeinali

Budiša

et al. 2022

Front. Bioeng. Biotechnol.

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“…(B) Schematic illustration of the fabrication of Fe 3 O 4 –PEI–pMaltose NPs and the selective enrichment of N‐ glycopeptides. Reprinted with permission of [94].…”

Section: Glycoprotein and Glycopeptide Enrichmentmentioning

confidence: 99%

Advances in mass spectrometry‐based glycoproteomics: An update covering the period 2017–2021

et al. 2021

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Protein glycosylation is one of the most common posttranslational modifications, and plays an essential role in a wide range of biological processes such as immune response, intercellular signaling, inflammation, host-pathogen interaction, and protein stability. Glycoproteomics is a proteomics subfield dedicated to identifying and characterizing the glycans and glycoproteins in a given cell or tissue. Aberrant glycosylation has been associated with various diseases such as Alzheimer's disease, viral infections, inflammation, immune deficiencies, congenital disorders, and cancers. However, glycoproteomic analysis remains challenging because of the low abundance, site-specific heterogeneity, and poor ionization efficiency of glycopeptides during LC-MS analyses. Therefore, the development of sensitive and accurate approaches to efficiently characterize protein glycosylation is crucial. Methods such as metabolic labeling, enrichment, and derivatization of glycopeptides, coupled with different mass spectrometry techniques and bioinformatics tools, have been developed to achieve sophisticated levels of quantitative and qualitative analyses of glycoproteins. This review attempts to update the recent developments in the field of glycoproteomics reported between 2017 and 2021.

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“…The resulting digests were desalted using Sep-pak C18 cartridges (Water Ltd., Elstree, UK), evaporated to dryness, and stored at −20 °C for later use. www.nature.com/scientificreports www.nature.com/scientificreports/ For the enrichment of N-glycopeptides from digests of human renal mesangial cells (HRMC, kindly donated by Dr. Mingzhen Li (Metabolic Diseases Hospital, Tianjin Medical University, Tianjin, China)), the operation was carried out according to a previous report 30 . Briefly, 200 μg of the sample was dissolved in 5 mL of loading buffer (ACN/H 2 O/TFA = 89:10.5:0.5, v/v/v), incubated with 20 mg of Fe 3 O 4 -GO@PDA-Chitosan nanocomposites for 1 h, and washed thrice with 2 mL of loading buffer.…”

Section: Preparation Of Fe 3 O 4 -Go@pda-chitosan Nanocomposites Intmentioning

confidence: 99%

Facile synthesis of hydrophilic magnetic graphene nanocomposites via dopamine self-polymerization and Michael addition for selective enrichment of N-linked glycopeptides

Yuan

et al. 2020

Sci Rep

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The development of methods to effectively capture N-glycopeptides from the complex biological samples is crucial to N-glycoproteome profiling. Herein, the hydrophilic chitosan-functionalized magnetic graphene nanocomposites (denoted as fe 3 o 4-Go@pDA-chitosan) were designed and synthesized via a simple two-step modification (dopamine self-polymerization and Michael addition). the fe 3 o 4-GO@PDA-Chitosan nanocomposites exhibited good performances with low detection limit (0.4 fmol•μL −1), good selectivity (mixture of bovine serum albumin and horseradish peroxidase tryptic digests at a molar ration of 10:1), good repeatability (4 times), high binding capacity (75 mg•g −1). Moreover, fe 3 o 4-Go@pDA-chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. This study provides a feasible strategy for the surface functionalized novel materials for isolation and enrichment of N-glycopeptides. Protein N-glycosylation, as one of the most prevalent post-translational modifications (PTMs), can significantly change the structure, increase stability and subsequently endow new functions of protein 1-3. Meanwhile, this modification plays determinant roles in physiological processes, such as molecular and cell recognition, signal transduction, immune response. Aberrant protein N-glycosylation is associated with human disease, such as rheumatoid arthritis, lupus erythematosus, Alzheimer's disease (AD) and so on 4-6 Therefore, the thorough analysis of protein N-glycosylation is beneficial for elucidating pathogenesis of many diseases and for the discovery of new clinical biomarkers and effective disease control 7. In the recent years, mass spectrometry (MS) has been widely used in N-glycoproteme analysis due to its high sensitivity and high-throughput and capacity to analyze disease-associated glycoforms 8. However, the low abundance of N-glycoproteins in biological samples, poor ionization of N-glycopeptides and the heterogeneity of glycan structures make the direct MS analysis extremely difficult 8. Therefore, an enrichment step of N-glycopeptides prior to MS analysis is a prerequisite for the successful glycoproteomics analysis. A variety of enrichment strategies towards glycoproteins/glycopeptides have been developed, such as boronic acid chemistry 9-12 , immobilized metal ion affinity chromatography (IMAC) 13-15 , molecularly imprinting

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Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides

Cited by 34 publications

References 42 publications

Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv

Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv

Advances in mass spectrometry‐based glycoproteomics: An update covering the period 2017–2021

Facile synthesis of hydrophilic magnetic graphene nanocomposites via dopamine self-polymerization and Michael addition for selective enrichment of N-linked glycopeptides

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