The highly specific enrichment of phosphoproteins and phosphopeptides from intricate biological systems is the precondition of in-depth phosphoproteome research. Herein, a novel dual-functionalized magnetic zirconium-based metal−organic framework (MOF) denoted as DFMMOF, with the purpose of combining the affinity of immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC) has been successfully synthesized. The inherent Zr−O cluster of DFMMOF particles acted as MOAC and the immobilized titanium(IV) ions served for IMAC. The obtained DFMMOF exhibited rapid magnetic separation (within 5 s), large surface area (237.9 m 2 g −1 ), high binding capacity (100 mg g −1 ), and good postenrichment recovery (84.8%). Thanks to the strong affinity, low detection sensitivity (5 fmol) and high selectivity (β-casein/BSA with a molar ratio of 1:1000) for phosphopeptide enrichment were obtained using DFMMOF as absorbent. Moreover, the effective identification of phosphopeptides from real samples (human serum and nonfat milk) further confirmed the immense potential of DFMMOF as a promising candidate for the detection and extraction of trace amounts of phosphorylated peptides in complex biosamples.

A novel multifunctional graphene/Fe3O4/TiO2 magnetic composite with excellent hydrophilicity and biological compatibility was synthesized and exhibited selective capture, fast magnetic isolation and sensitive analysis of low-abundance phosphopeptides from complex biosamples.

Microcystins(MCs), a family of potently cyclic heptapeptides, are produced by cyanobacteria blooms in eutrophic water and can cause acute and chronic toxicity even mortality to animal and humans. There existed some strategies for removal of MCs, which only concerned about the highly contaminated water whose concentration of the pollutant was considerably larger than that in 10 nature world. Herein, we developed a ternary composite of TiO 2 -coated magnetic graphene and used it as adsorbent and photocatalyst to remove microcystin-LR (MC-LR) from water efficiently. The two-dimensional sheets of graphene were decorated by a large quantity of spherical Fe 3 O 4 nanoparticles (10-20 nm) and then coated by crystallized TiO 2 . These TiO 2 -graphene@Fe 3 O 4 composites exhibited a high magnetic response to external magnetic field. And the huge surface of 15 graphene dramatically boost the adsorbability and the charge mobility which lowers the recombination rate of electron-hole pairs and hence systematically enhances the photocatalytic activity. The combination of adsorption and photodegradation provide the composite better performance on the removal of trace amout of MC-LR than commercial photocatalyst Degussa P25. The concentration of MC-LR can be lowered to less than 1µg L -1 (a provisional safety guideline by 20 WHO) from 500µg L -1 under UV light in 30 min. The loading of TiO 2 -graphene@Fe 3 O 4 , pH and UV energy were also optimized. Moreover, the stable removal capability of TiO 2 -graphene@Fe 3 O 4 were comfirmed over multiple recycling. Finally the removing performance was also evaluated under solar light illumination in real surface water samples. This work would pave the way for the development of more efficient and easily separable purifier to remove pollutants and toxins in 25 contaminated water.

A kind of novel multi-stimuli responsive molecularly imprinted polymers with bovine serum protein (BSA) as dummy template (MSR-BSA-MIPs) was fabricated for specific recognition of human serum protein (HSA) with modulated...