Dual-Functionalized Magnetic Metal–Organic Framework for Highly Specific Enrichment of Phosphopeptides

Zhou, Jianqiao; Liang, Yulu; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1021/acssuschemeng.7b02521

Cited by 98 publications

(52 citation statements)

References 51 publications

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“…Recently, nucleotides such as adenosine triphosphate (ATP) have been also incorporated in MOFs, taking advantage of its phosphate functionalities for immobilizing metal ions. Thus, Zhou et al [ 77 ] have synthesized a dual-functionalized magnetic zirconium-based MOF for the selective extraction of phosphopeptides. The biocomposite was prepared by modifying UiO-66-NH 2 MOF onto PDA-coated magnetic spheres and subsequently the immobilization of titanium (IV) ions onto the surface of the obtained ATP-grafted magnetic MOF (see synthetic route in Figure 7 A).…”

Section: Applications Of Affinity-based Mof Materials In Sample Trmentioning

confidence: 99%

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Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

2020

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This review summarizes the recent advances concerning metal–organic frameworks (MOFs) modified with several biomolecules (e.g., amino acids, nucleobases, proteins, antibodies, aptamers, etc.) as ligands to prepare affinity-based sorbents for application in the sample preparation field. The preparation and incorporation strategies of these MOF-based affinity materials were described. Additionally, the different types of ligands that can be employed for the synthesis of these biocomposites and their application as sorbents for the selective extraction of molecules and clean-up of complex real samples is reported. The most important features of the developed biocomposites will be discussed throughout the text in different sections, and several examples will be also commented on in detail.

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Section: Applications Of Affinity-based Mof Materials In Sample Trmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

2020

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“…Furthermore, the MOF of UiO‐66–NH 2 was also employed to construct novel nanomaterials with hybrid model of IMAC and MOAC. Zhou et al immobilized Ti(IV) ions onto UiO‐66–NH 2 by using ATP as linker (Figure C), which exhibited 100 mg g −1 of binding capacity and 84.8% of enrichment recovery . Peng et al immobilized Zr(IV) ions onto UiO‐66–NH 2 by using POCl 3 as linker (dubbed DZMOF, Figure D), and identified 17 phosphopeptides (5 mono‐ and 12 multiphosphopeptides) from 5 µL human saliva .…”

Section: Nanomaterials In Phosphoproteomicsmentioning

confidence: 99%

Nanomaterials in Proteomics

Sun

Shen

et al. 2019

Adv Funct Materials

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For a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.

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“…Recently, Functionalized MOFs are investigated in specific enrichment of phosphopeptides [36,37]. For instance, dual-functionalized MOFs, combining the affinity of immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC), were synthesized [38]. The Zr-O cluster acted as MOAC and immobilized Ti 4+ served as IMAC.…”

Section: Phosphoprotein/phosphopeptide Enrichment or Phosphate Recmentioning

confidence: 99%

Recent Advances in Protein Kinase Activity Analysis Based on Nanomaterials

Deng

Liu

2019

IJMS

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Protein phosphorylation regulated by protein kinases, as well as their dephosphorylation, is one of the most common post-translational modifications, and plays important roles in physiological activities, such as intracellular signal communications, gene transcription, cell proliferation and apoptosis. Over-expression of protein kinases is closely associated with various diseases. Consequently, accurate detection of protein kinases activities and their relevant inhibitors screening is critically important, not only to the biochemical research, but also to the clinical diagnosis and therapy. Nanomaterials, taking advantage of large surface areas, as well as excellent electrical, catalytic, magnetic and optical properties, have been utilized as target concentrators, recognition components, signal transducer or amplification elements in protein kinase related assays. This review summarizes the recent representative works to highlight the applications of nanomaterials in different biosensor technologies for protein kinases activities detection and their inhibitors screening. First, different nanomaterials developed for phosphoprotein/phosphopeptide enrichment and phosphate recognition are introduced. Next, representative works are selected that mainly focus on the utilization of nanomaterials as signal transducer or amplification elements in various protein kinases sensing platforms, such as electrochemical, colorimetric, fluorescent, and mass spectroscopy-based approaches. Finally, the major challenges and perspectives of nanomaterials being applied in protein kinases related assays are discussed.

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Dual-Functionalized Magnetic Metal–Organic Framework for Highly Specific Enrichment of Phosphopeptides

Cited by 98 publications

References 51 publications

Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes

Nanomaterials in Proteomics

Recent Advances in Protein Kinase Activity Analysis Based on Nanomaterials

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